1.13.12.19: 2-oxoglutarate dioxygenase (ethene-forming)
This is an abbreviated version!
For detailed information about 2-oxoglutarate dioxygenase (ethene-forming), go to the full flat file.
Word Map on EC 1.13.12.19
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1.13.12.19
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1-aminocyclopropane-1-carboxylic
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2og-feii
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syringae
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climacteric
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carnation
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dianthus
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photoautotrophic
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biotechnology
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agriculture
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synthesis
- 1.13.12.19
-
1-aminocyclopropane-1-carboxylic
-
2og-feii
- syringae
-
climacteric
- carnation
-
dianthus
-
photoautotrophic
- biotechnology
- agriculture
- synthesis
Reaction
Synonyms
2-oxoglutarate-Fe(II) oxygenase, 2OG-Fe(II) oxygenase, EFE, ethylene forming enzyme, ethylene-forming enzyme, More, PsEFE
ECTree
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Crystallization
Crystallization on EC 1.13.12.19 - 2-oxoglutarate dioxygenase (ethene-forming)
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analysis of crystal structure of the enzyme in complex with several ligands, detailed overview
purified recombinant enzyme in three structures: of enzyme in complex with manganese and 2-oxoglutarate, of enzyme in complex with with manganese and bis-Tris-propane buffer, and enzyme in complex with iron, L-Arg, and N-oxalylglycine, hanging drop vapour diffusion method, mixing of 0.003 ml of 12-20 mg/ml protein solution with 0.003 ml of reservoir solution containing 17.5-25% w/v PEG 3350 and PEG 6000, 0.1 M bis-Tris-propane, pH 7.0, 0.04 M sodium/potassium phosphate, 3-5 mM MnCl2, and with or without 10 mM 2-oxoglutarate, and equilibration against 0.5 ml reservoir solution, at 20°, microseeding, method optimization, X-ray diffraction structure determination and analysis at 1.08-1.55 A resolution, single-wavelength anomalous diffraction data obtained from selenomethionine-derivatized PsEFE:Mn:bis-Tris-propane crystals, structure modeling