EC Number |
Protein Variants |
Reference |
---|
3.4.22.53 | C105S |
inactive mutant enzyme |
644016 |
3.4.22.53 | C105S |
inactive mutant enzyme. The mutant enzyme provides a purified calpain, that is stable to autolysis and oxidation, which is likely to facilitate crystallization in both the presence and absence of calcium |
644028 |
3.4.22.53 | C105S |
mutant enzyme of mutant large subunit m-C105S-80K, coexpressed with 30000 Da subunit in Sf-9 cells does not degrade casein nor the artificial substrate succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide. The mutant enzyme does not show autolytic activity with Ca2+ |
644029 |
3.4.22.53 | D346E |
mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation |
696327 |
3.4.22.53 | D362K |
mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation |
696327 |
3.4.22.53 | E504S |
Ca2+ concentration required for half-maximal activity is 0.129 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme |
644019 |
3.4.22.53 | E504S |
mutation decreases specific activity to 90% compared to wild-type enzyme |
644019 |
3.4.22.53 | G423R |
mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation |
696327 |
3.4.22.53 | H262A |
inactive mutant enzyme |
644016 |
3.4.22.53 | K225S |
mutation decreases specific activity to 88% compared to wild-type enzyme |
644019 |