EC Number |
Substrates |
Products |
Reversibility |
---|
1.4.99.6 | (3S,3R)-2-oxo-3-methylvaleric acid + NH3 + NADPH + H+ |
- |
D-isoleucine + H2O + NADP+ |
yield of 45% after incubation for 1 h at 50 °C |
? |
1.4.99.6 | 2-oxo-3-methylbutyric acid + NH3 + NADPH + H+ |
- |
D-valine + H2O + NADP+ |
yield of 83% after incubation for 1 h at 50 °C |
? |
1.4.99.6 | 2-oxo-4-methylvaleric acid + NH3 + NADPH + H+ |
- |
D-leucine + H2O + NADP+ |
yield of 91% after incubation for 1 h at 50 °C |
? |
1.4.99.6 | D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol |
- |
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol |
- |
? |
1.4.99.6 | D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol |
36.8% activity compared to D-Pro |
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol |
- |
? |
1.4.99.6 | D-alanine + H2O + 2,6-dichlorophenolindophenol |
- |
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol |
- |
? |
1.4.99.6 | D-alanine + H2O + 2,6-dichlorophenolindophenol |
low activity |
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol |
- |
? |
1.4.99.6 | D-alanine + H2O + FAD |
D-amino acid dehydrogenase activity of broad substrate specificity |
pyruvate + NH3 + FADH2 |
- |
? |
1.4.99.6 | D-alanine + H2O + FAD |
the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity |
pyruvate + NH3 + FADH2 |
- |
? |
1.4.99.6 | D-alanine + H2O + methylene blue |
low activity |
pyruvate + NH3 + reduced methylene blue |
- |
? |