3.4.22.B50 | ubiquitin-7-amido-4-trifluoromethylcoumarin + H2O |
the enzyme catalyzes proteolytic processing of the viral polyprotein and also shows significant in vitro deubiquitinating and de-ISGylating activities. The enzyme binds ubiquitin, the ubiquitin core makes mostly hydrophilic interactions with the enzyme, while the Leu-Arg-Gly-Gly C-terminus of ubiquitin is located in the catalytic cleft of the enzyme. The ubiquitin core binds to the palm, thumb and fingers domains of the enzyme, while its final four C-terminal residues bind into a narrow channel by a network of hydrogen bonds and reach towards the active site |
Severe acute respiratory syndrome-related coronavirus |
ubiquitin + 7-amino-4-trifluoromethylcoumarin |
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