0.01
-
ubiquitin-7-amido-4-trifluoromethylcoumarin
recombinant mutant Y265A, pH 7.4, 30°C
0.045
-
RLRGG-7-amido-4-methylcoumarin
wild type enzyme, at pH 8.0 and 37°C
0.055
-
Dabcyl-FRLKGGAPIKGV-Edans
mutant T39W, pH 6.5, 30°C
0.058
-
Dabcyl-FRLKGGAPIKGV-Edans
wild-type, pH 6.5, 30°C
0.065
-
RLRGG-7-amido-4-methylcoumarin
mutant enzyme I353R, at pH 8.0 and 37°C
0.08
-
ubiquitin-7-amido-4-trifluoromethylcoumarin
recombinant mutant D165A, pH 7.4, 30°C
0.082
-
Dabcyl-FKKKGGGDVKE-Edans
wild-type, pH 6.5, 30°C
0.103
-
Dabcyl-FKKKGGGDVKE-Edans
mutant T39W, pH 6.5, 30°C
0.155
-
RLRGG-7-amido-4-methylcoumarin
mutant enzyme I353W, at pH 8.0 and 37°C
0.23
-
ubiquitin-7-amido-4-trifluoromethylcoumarin
recombinant mutant E168A, pH 7.4, 30°C
