EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.4.22.B50 | 0.36 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant L163Q, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 0.413 |
- |
ubiquitin-aminomethylcoumarin |
mutant enzyme I353R, at pH 8.0 and 37°C |
732827 |
3.4.22.B50 | 0.45 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant Y265F, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 0.55 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant E168D, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 1.01 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant E168R, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 1.08 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant wild-type enzyme, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 1.741 |
- |
ubiquitin-aminomethylcoumarin |
mutant enzyme I353W, at pH 8.0 and 37°C |
732827 |
3.4.22.B50 | 1.8 |
- |
N-benzoyl-Phe-Val-Arg-4-nitroanilide |
wild type enzyme, in 50 mM Na-acetate buffer pH 5.0, at 60°C |
732725 |
3.4.22.B50 | 14.75 |
- |
N-benzoyl-Phe-Val-Arg-4-nitroanilide |
mutant enzyme A67Y, in 50 mM Na-acetate buffer pH 5.0, at 60°C |
732725 |
3.4.22.B50 | 14.76 |
- |
N-benzoyl-Phe-Val-Arg-4-nitroanilide |
mutant enzyme S32T/A67Y, in 50 mM Na-acetate buffer pH 5.0, at 60°C |
732725 |