3.4.22.B50 (E-EDANS)RELNGGAPI(K-DABCYL)S + H2O - Alphacoronavirus ? - ? 383468 3.4.22.B50 (E-EDANS)RELNGGAPI(K-DABCYL)S + H2O - Alphacoronavirus SARS-CoV ? - ? 383468 3.4.22.B50 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O fluorogenic substrate Alphacoronavirus ? - ? 384049 3.4.22.B50 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O fluorogenic substrate Alphacoronavirus SARS-CoV ? - ? 384049 3.4.22.B50 azocasein + H2O - Tabernaemontana divaricata ? - ? 15347 3.4.22.B50 BCoV substrate - Alphacoronavirus ? - ? 384255 3.4.22.B50 BCoV substrate - Alphacoronavirus SARS-CoV ? - ? 384255 3.4.22.B50 branched polyubiquitin chains + H2O - Alphacoronavirus ? - ? 384371 3.4.22.B50 branched polyubiquitin chains + H2O - Alphacoronavirus SARS-CoV ? - ? 384371 3.4.22.B50 Dabcyl-FKKKGGGDVKE-Edans + H2O - porcine epidemic diarrhea virus ? - ? 449823 3.4.22.B50 Dabcyl-FRLKGGAPIKGV-Edans + H2O SARS-CoV-derived substrate porcine epidemic diarrhea virus ? - ? 449825 3.4.22.B50 diubiquitin + H2O - Alphacoronavirus ? - ? 384619 3.4.22.B50 diubiquitin + H2O - Alphacoronavirus SARS-CoV ? - ? 384619 3.4.22.B50 FRLKGG-4-nitroanilide + H2O - Severe acute respiratory syndrome-related coronavirus FRLKGG + 4-nitroaniline - ? 431859 3.4.22.B50 FRLKGGAPIKGV 12 mer oligopeptide containing Gly818-Ala819, 100% cleavage Alphacoronavirus ? - ? 384728 3.4.22.B50 FRLKGGAPIKGV 12 mer oligopeptide containing Gly818-Ala819, 100% cleavage Alphacoronavirus SARS-CoV ? - ? 384728 3.4.22.B50 FTKLAGGKISFS + H2O - Human coronavirus NL63 FTKLAG + GKISFS - ? 417951 3.4.22.B50 Gelatin + H2O - Tabernaemontana divaricata ? - ? 16570 3.4.22.B50 ISG15-nsp2-fusion protein + H2O - Alphacoronavirus ? - ? 384933 3.4.22.B50 ISG15-nsp2-fusion protein + H2O - Alphacoronavirus SARS-CoV ? - ? 384933 3.4.22.B50 ISLKGGKIVSTC 12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage Alphacoronavirus ? - ? 384934 3.4.22.B50 ISLKGGKIVSTC 12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage Alphacoronavirus SARS-CoV ? - ? 384934 3.4.22.B50 MHV substrate - Alphacoronavirus ? - ? 385188 3.4.22.B50 additional information Cys1715 is a catalytic residue of PLP2 Murine coronavirus ? - ? 89 3.4.22.B50 additional information no in vitro cleavage of the ORF 1a polyprotein in cis or in trans can be detected with PLP-2 expressed either as a polypeptide, including flanking viral sequences, or as an MBP fusion protein Murine coronavirus ? - ? 89 3.4.22.B50 additional information the enzyme is one of three distinct viral proteases (PLP1, PLP2 and 3CLpro) involved in processing of the replicase polyprotein Murine coronavirus ? - ? 89 3.4.22.B50 additional information SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates Alphacoronavirus ? - ? 89 3.4.22.B50 additional information proteolytic processing of the human coronavirus 229E. PL2pro is able to cleave the nsp1-nsp2 cleavage site. PL2pro plays a universal and essential proteolytic role that appears to be assisted by the PL1pro paralog at specific sites Human coronavirus 229E ? - ? 89 3.4.22.B50 additional information the core domain of PLP2 has in vivo deubiquitinase and DeISGylation activity Human coronavirus NL63 ? - ? 89 3.4.22.B50 additional information the enzyme cannot cleave Z-KAGG-7-amido-4-methylcoumarin infectious bronchitis virus ? - ? 89 3.4.22.B50 additional information SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates Alphacoronavirus SARS-CoV ? - ? 89 3.4.22.B50 N-benzoyl-Phe-Val-Arg-4-nitroanilide + H2O - Tabernaemontana divaricata N-benzoyl-Phe-Val-Arg + 4-nitroaniline - ? 74217 3.4.22.B50 PLP2-MP1 precursor polyprotein + H2O - Alphacoronavirus MP1 + ? - ? 385562 3.4.22.B50 polyubiquitin + H2O the enzyme cleaves Lys48- and Lys63-linked polyubiquitin to monoubiquitin but not linear polyubiquitin infectious bronchitis virus monoubiquitin + ? - ? 431114 3.4.22.B50 RELNGGAVTRYV + H2O 12 mer oligopeptide containing Gly180-Ala181, 5% cleavage Alphacoronavirus AVTYRV + RELNGG - ? 385671 3.4.22.B50 replicase polyprotein + H2O PLP2 cleaves a substrate encoding the first predicted membrane-spanning domain, MP1, of the replicase polyprotein. Processing the replicase polyprotein at this site generates the p150 replicase intermediate that is likely critical for embedding the replicase complex into cellular membranes. The enzyme acts efficiently in trans Murine coronavirus ? - ? 362309 3.4.22.B50 RLRGG-7-amido-4-methylcoumarin + H2O - equine arteritis virus RLRGG + 7-amino-4-methylcoumarin - ? 432349 3.4.22.B50 ubiquitin + H2O - equine arteritis virus ? - ? 355649 3.4.22.B50 ubiquitin + H2O the enzyme cleaves the LRGG tail of ubiquitin Human coronavirus NL63 ? - ? 355649 3.4.22.B50 ubiquitin + H2O the enzyme processes both K-48 and K-63 linked polyubiquitin chains porcine epidemic diarrhea virus ? - ? 355649 3.4.22.B50 ubiquitin-7-amido-4-methylcoumarin + H2O - Alphacoronavirus ? - ? 385871 3.4.22.B50 ubiquitin-7-amido-4-methylcoumarin + H2O - Alphacoronavirus SARS-CoV ? - ? 385871 3.4.22.B50 ubiquitin-7-amido-4-trifluoro-methyl-coumarin + H2O - porcine epidemic diarrhea virus ubiquitin + 7-amino-4-trifluoro-methyl-coumarin - ? 450791 3.4.22.B50 ubiquitin-7-amido-4-trifluoromethylcoumarin + H2O the enzyme catalyzes proteolytic processing of the viral polyprotein and also shows significant in vitro deubiquitinating and de-ISGylating activities. The enzyme binds ubiquitin, the ubiquitin core makes mostly hydrophilic interactions with the enzyme, while the Leu-Arg-Gly-Gly C-terminus of ubiquitin is located in the catalytic cleft of the enzyme. The ubiquitin core binds to the palm, thumb and fingers domains of the enzyme, while its final four C-terminal residues bind into a narrow channel by a network of hydrogen bonds and reach towards the active site Severe acute respiratory syndrome-related coronavirus ubiquitin + 7-amino-4-trifluoromethylcoumarin - ? 432450 3.4.22.B50 ubiquitin-aminomethylcoumarin + H2O - equine arteritis virus ? - ? 432451 3.4.22.B50 ubiquitinated branched peptides + H2O - Human coronavirus NL63 ? - ? 419162 3.4.22.B50 ubiquitinated RIG-I + H2O - porcine epidemic diarrhea virus deubiquitinated RIG-I + ubiquitin - ? 431230 3.4.22.B50 ubiquitinated STING + H2O - porcine epidemic diarrhea virus deubiquitinated STING + ubiquitin - ? 431231 3.4.22.B50 VAKQGAGFKRTY + H2O - Human coronavirus NL63 VAKQGA + GFKRTY - ? 418118 3.4.22.B50 viral replicase polyprotein + H2O PLP2 is responsible for processing both cleavage sites 2 and 3 to release nsp2 and nsp3. The cleavage sites are identified as FTKLAG-/-GKISFS for CS2 and VAKQGA-/-GFKRTY for CS3. Human coronavirus NL63 ? - ? 393827 3.4.22.B50 Z-KKAG-7-amido-4-methylcoumarin + H2O the catalytic efficiency toward Z-KKAG-7-amido-4-methylcoumarin is 5times higher than that for Z-LRGG-7-amido-4-methylcoumarin infectious bronchitis virus Z-KKAG + 7-amino-4-methylcoumarin - ? 432499 3.4.22.B50 Z-LRGG-7-amido-4-methylcoumarin + H2O - Alphacoronavirus ? - ? 385964 3.4.22.B50 Z-LRGG-7-amido-4-methylcoumarin + H2O - Alphacoronavirus SARS-CoV ? - ? 385964 3.4.22.B50 Z-LRGG-7-amido-4-methylcoumarin + H2O - infectious bronchitis virus Z-LRGG + 7-amino-4-methylcoumarin - ? 432502