3.4.22.1 2,4-dinitrophenyl-GARFW-OH + H2O - Homo sapiens ? - ? 366640 3.4.22.1 2,4-dinitrophenyl-GDRFW-OH + H2O - Homo sapiens ? - ? 366649 3.4.22.1 2,4-dinitrophenyl-GERFW-OH + H2O - Homo sapiens ? - ? 366648 3.4.22.1 2,4-dinitrophenyl-GFAFW-OH + H2O - Homo sapiens ? - ? 366626 3.4.22.1 2,4-dinitrophenyl-GFDFW-OH + H2O - Homo sapiens ? - ? 366635 3.4.22.1 2,4-dinitrophenyl-GFEFW-OH + H2O - Homo sapiens ? - ? 366634 3.4.22.1 2,4-dinitrophenyl-GFFFW-OH + H2O - Homo sapiens ? - ? 366624 3.4.22.1 2,4-dinitrophenyl-GFGFW-OH + H2O - Homo sapiens ? - ? 366630 3.4.22.1 2,4-dinitrophenyl-GFHFW-OH + H2O - Homo sapiens ? - ? 366638 3.4.22.1 2,4-dinitrophenyl-GFIFW-OH + H2O - Homo sapiens ? - ? 366629 3.4.22.1 2,4-dinitrophenyl-GFKFW-OH + H2O - Homo sapiens ? - ? 366636 3.4.22.1 2,4-dinitrophenyl-GFLFW-OH + H2O - Homo sapiens ? - ? 366628 3.4.22.1 2,4-dinitrophenyl-GFPFW-OH + H2O - Homo sapiens ? - ? 366631 3.4.22.1 2,4-dinitrophenyl-GFQFW-OH + H2O - Homo sapiens ? - ? 366632 3.4.22.1 2,4-dinitrophenyl-GFRAW-OH + H2O - Homo sapiens ? - ? 366654 3.4.22.1 2,4-dinitrophenyl-GFRDW-OH + H2O - Homo sapiens ? - ? 366663 3.4.22.1 2,4-dinitrophenyl-GFREW-OH + H2O - Homo sapiens ? - ? 366662 3.4.22.1 2,4-dinitrophenyl-GFRFW-OH + H2O - Homo sapiens ? - ? 366637 3.4.22.1 2,4-dinitrophenyl-GFRGW-OH + H2O - Homo sapiens ? - ? 366658 3.4.22.1 2,4-dinitrophenyl-GFRHW-OH + H2O - Homo sapiens ? - ? 366666 3.4.22.1 2,4-dinitrophenyl-GFRIW-OH + H2O - Homo sapiens ? - ? 366657 3.4.22.1 2,4-dinitrophenyl-GFRKW-OH + H2O - Homo sapiens ? - ? 366664 3.4.22.1 2,4-dinitrophenyl-GFRLW-OH + H2O - Homo sapiens ? - ? 366656 3.4.22.1 2,4-dinitrophenyl-GFRPW-OH + H2O - Homo sapiens ? - ? 366659 3.4.22.1 2,4-dinitrophenyl-GFRQW-OH + H2O - Homo sapiens ? - ? 366660 3.4.22.1 2,4-dinitrophenyl-GFRRW-OH + H2O - Homo sapiens ? - ? 366665 3.4.22.1 2,4-dinitrophenyl-GFRSW-OH + H2O - Homo sapiens ? - ? 366661 3.4.22.1 2,4-dinitrophenyl-GFRVW-OH + H2O - Homo sapiens ? - ? 366655 3.4.22.1 2,4-dinitrophenyl-GFRW R-OH + H2O - Homo sapiens ? - ? 453073 3.4.22.1 2,4-dinitrophenyl-GFRWA-OH + H2O - Homo sapiens ? - ? 366669 3.4.22.1 2,4-dinitrophenyl-GFRWD-OH + H2O - Homo sapiens ? - ? 453070 3.4.22.1 2,4-dinitrophenyl-GFRWE-OH + H2O - Homo sapiens ? - ? 453071 3.4.22.1 2,4-dinitrophenyl-GFRWF-OH + H2O - Homo sapiens ? - ? 366667 3.4.22.1 2,4-dinitrophenyl-GFRWG-OH + H2O - Homo sapiens ? - ? 366673 3.4.22.1 2,4-dinitrophenyl-GFRWI-OH + H2O - Homo sapiens ? - ? 366672 3.4.22.1 2,4-dinitrophenyl-GFRWK-OH + H2O - Homo sapiens ? - ? 453072 3.4.22.1 2,4-dinitrophenyl-GFRWL-OH + H2O - Homo sapiens ? - ? 366671 3.4.22.1 2,4-dinitrophenyl-GFRWP-OH + H2O - Homo sapiens ? - ? 366674 3.4.22.1 2,4-dinitrophenyl-GFRWQ-OH + H2O - Homo sapiens ? - ? 366675 3.4.22.1 2,4-dinitrophenyl-GFRWS-OH + H2O - Homo sapiens ? - ? 453074 3.4.22.1 2,4-dinitrophenyl-GFRWV-OH + H2O - Homo sapiens ? - ? 366670 3.4.22.1 2,4-dinitrophenyl-GFRWY-OH + H2O - Homo sapiens ? - ? 366668 3.4.22.1 2,4-dinitrophenyl-GFRYW-OH + H2O - Homo sapiens ? - ? 366653 3.4.22.1 2,4-dinitrophenyl-GFSFW-OH + H2O - Homo sapiens ? - ? 366633 3.4.22.1 2,4-dinitrophenyl-GFVFW-OH + H2O - Homo sapiens ? - ? 366627 3.4.22.1 2,4-dinitrophenyl-GFYFW-OH + H2O - Homo sapiens ? - ? 366625 3.4.22.1 2,4-dinitrophenyl-GGRFW-OH + H2O - Homo sapiens ? - ? 366644 3.4.22.1 2,4-dinitrophenyl-GHRFW-OH + H2O - Homo sapiens ? - ? 366652 3.4.22.1 2,4-dinitrophenyl-GIRFW-OH + H2O - Homo sapiens ? - ? 366643 3.4.22.1 2,4-dinitrophenyl-GKRFW-OH + H2O - Homo sapiens ? - ? 366650 3.4.22.1 2,4-dinitrophenyl-GLRFW-OH + H2O - Homo sapiens ? - ? 366642 3.4.22.1 2,4-dinitrophenyl-GPRFW-OH + H2O - Homo sapiens ? - ? 366645 3.4.22.1 2,4-dinitrophenyl-GQRFW-OH + H2O - Homo sapiens ? - ? 366646 3.4.22.1 2,4-dinitrophenyl-GRRFW-OH + H2O - Homo sapiens ? - ? 366651 3.4.22.1 2,4-dinitrophenyl-GSRFW-OH + H2O - Homo sapiens ? - ? 366647 3.4.22.1 2,4-dinitrophenyl-GVRFW-OH + H2O - Homo sapiens ? - ? 366641 3.4.22.1 2,4-dinitrophenyl-GYRFW-OH + H2O - Homo sapiens ? - ? 366639 3.4.22.1 2-aminobenzoyl-Gly-Ile-Val-Arg-Ala-(Nepsilon-2,4-dinitrophenyl)Lys + H2O an exoproteolytic substrate Homo sapiens 2-aminobenzoyl-Gly-Ile-Val-Arg + Ala-(Nepsilon-2,4-dinitrophenyl)Lys - ? 406253 3.4.22.1 2-aminobenzoyl-Gly-Ile-Val-Arg-Ala-Lys(Dnp)-OH + H2O exopeptidase activity Homo sapiens ? - ? 433175 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-IEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O no hydrolysis at pH 6.0 Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-IEGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366618 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-L-Arg-L-Leu-L-Arg-Gly-L-Phe-D-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-L-Arg-L-Leu-L-Arg-Gly-L-Phe-D-Glu + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366613 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-L-Arg-L-Leu-L-Arg-Gly-L-Phe-L-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-L-Arg-L-Leu-L-Arg-Gly-L-Phe-L-Glu + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366612 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-L-Leu-L-Arg-Gly-L-Phe-D-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-L-Leu-L-Arg-Gly-L-Phe-D-Glu + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366615 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-L-Leu-L-Arg-Gly-L-Phe-L-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-L-Leu-L-Arg-Gly-L-Phe-L-Glu + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366614 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-LEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O no hydrolysis at pH 6.0 Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-LEGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366620 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-LRGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-LRGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366621 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-RIEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-RIEGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366617 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-RIIEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O no hydrolysis at pH 6.0 Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-RIIEGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366616 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-RLEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-RLEGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366619 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-RLVG-beta-(2-naphthyl)alanine-E-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-RLVG-beta-(2-naphthyl)alanine-E + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366609 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-RLVGFD-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-RLVGFD + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366611 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-RLVGFE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-RLVGFE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366607 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-RLVGFL-alpha-aminoadipic acid-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-RLVGFL-alpha-aminoadipic acid + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366610 3.4.22.1 4-(4-dimethylaminophenylazo)benzoyl-RLVGWE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Bos taurus 4-(4-dimethylaminophenylazo)benzoyl-RLVGWE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 366608 3.4.22.1 5-amino-1-(phenylsulfonyl)-1H-pyrazol-3-yl thiophene-2-carboxylate + H2O - Homo sapiens 5-amino-1-(phenylsulfonyl)-1,2-dihydro-3H-pyrazol-3-one + thiophene-2-carbaldehyde - ? 391006 3.4.22.1 5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl pyridine-4-carboxylate + H2O - Homo sapiens 5-amino-1-[(4-methoxyphenyl)sulfonyl]-1,2-dihydro-3H-pyrazol-3-one + pyridine-4-carbaldehyde - ? 391007 3.4.22.1 5-dimethylaminonaphthalene-1-sulfonyl-Phe-Arg-Phe(NO2)-Leu + H2O pH 6.4, 37°C, 2 mM cysteine, 1 mM EDTA Bos taurus ? - ? 383954 3.4.22.1 6-maleimidocaproic acid-L-Arg-Arg-Ala-Leu-Ala-Leu-Ala-camptothecin + H2O - Homo sapiens ? major cleavage products are L-Leu-Ala-Leu-Ala-camptothecin, L-Ala-Leu-Ala-camptothecin, L-Leu-Ala-camptothecin, and camptothecin ? 391047 3.4.22.1 6-maleimidocaproic acid-L-Arg-Arg-Ala-Leu-Ala-Leu-doxorubicin + H2O - Homo sapiens ? major cleavage products are L-Leu-Ala-Leu-doxorubicin, L-Leu-doxorubicin, and doxorubicin ? 391048 3.4.22.1 Abeta peptide + H2O the enzyme cleaves the Abeta peptide from the carboxylic end of Glu11 Hordeum vulgare ? - ? 449416 3.4.22.1 Abz-Ala-Phe-Arg-Ser-Ala-X-Gln-EDDnp + H2O kcat/Km for Abz-Ala-Phe-Arg-Ser-Ala-X-Gln-EDDnp, with X is Asn > Tyr, His, Leu, Trp > Ser, Phe, Pro Lys > Asp, Ala Homo sapiens Abz-Ala-Phe-Arg + Ser-Ala-X-Gln-EDDnp - ? 449424 3.4.22.1 Abz-Ala-Phe-Arg-Ser-X-Arg-Ser-Ala-Gln-EDDnp + H2O kcat/Km for Abz-Ala-Phe-Arg-Ser-X-Arg-Ser-Ala-Gln-EDDnp, with X is Asn > Trp, Tyr, Ala > Ser, Phe > His, Lys > Asp, Leu, Pro Homo sapiens Abz-Ala-Phe-Arg + Ser-X-Arg-Ser-Ala-Gln-EDDnp - ? 449425 3.4.22.1 Abz-Ala-X-Ala-Phe-Arg-Ser-Ala-Gln-EDDnp + H2O kcat/Km for Abz-Ala-X-Ala-Phe-Arg-Ser-Ala-Gln-EDDnp, with X is Leu, Tyr > Phe, Ala, Ser, Pro > His, Lys > Asn, Asp Homo sapiens Abz-Ala-X-Ala-Phe-Arg + Ser-Ala-Gln-EDDnp - ? 449429 3.4.22.1 Abz-Ala-X-Arg-Ser-Ala-Ala-Gln-EDDnp + H2O kcat/Km for Abz-Ala-X-Arg-Ser-Ala-Ala-Gln-EDDnp, with X is Phe, Leu > Arg > Ala > Pro Homo sapiens Abz-Ala-X-Arg + Ser-Ala-Ala-Gln-EDDnp - ? 449430 3.4.22.1 Abz-FF-3-dinitrophenyl-2,3-diaminopropionic acid-W-OH + H2O - Homo sapiens ? - ? 453075 3.4.22.1 Abz-FR-3-dinitrophenyl-2,3-diaminopropionic acid-W-OH + H2O - Homo sapiens ? - ? 453076 3.4.22.1 Abz-FR-epsilon-dinitrophenyl-L-lysyl-2,3-diaminopropionic acid-P-OH + H2O - Homo sapiens ? - ? 453077 3.4.22.1 Abz-FR-epsilon-dinitrophenyl-L-lysyl-2,3-diaminopropionic acid-W-OH + H2O - Homo sapiens ? - ? 453078 3.4.22.1 Abz-FRA-epsilon-dinitrophenyl-L-lysyl-2,3-diaminopropionic acid-OH + H2O - Homo sapiens ? - ? 453079 3.4.22.1 Abz-FRF(NO2)A + H2O - Nicotiana benthamiana ? - ? 449439 3.4.22.1 Abz-FRF-epsilon-dinitrophenyl-L-lysyl-2,3-diaminopropionic acid-OH + H2O - Homo sapiens ? - ? 453080 3.4.22.1 Abz-GIVRAK(Dnp)-OH + H2O 200 mM NaCl, 2.5 mM DTT, pH 4.5, 37°C Homo sapiens ? - ? 384050 3.4.22.1 Abz-GIVRAK-Dnp + H2O - Nicotiana benthamiana ? - ? 449443 3.4.22.1 Abz-GXXRZK(Dnp)-OH + H2O 200 mM NaCl, 2.5 mM DTT, pH 4.5, 37°C Homo sapiens ? - ? 384051 3.4.22.1 Abz-GXXZXK(Dnp)-OH + H2O 200 mM NaCl, 2.5 mM DTT, pH 4.5, 37°C Homo sapiens ? - ? 384052 3.4.22.1 Abz-GXZRXK(Dnp)-OH + H2O 200 mM NaCl, 2.5 mM DTT, pH 4.5, 37°C Homo sapiens ? - ? 384053 3.4.22.1 Abz-GZXRXK(Dnp)-OH + H2O 200 mM NaCl, 2.5 mM DTT, pH 4.5, 37°C Homo sapiens ? - ? 384054 3.4.22.1 Abz-X-Ala-Phe-Arg-Ser-Ala-Gln-EDDnp + H2O kcat/Km for Abz-X-Ala-Phe-Arg-Ser-Ala-Gln-EDDnp, with X is Leu, Phe, Trp > Ser, Asn, Asp, Ala > Tyr, His, Lys Homo sapiens Abz-X-Ala-Phe-Arg + Ser-Ala-Gln-EDDnp - ? 449473 3.4.22.1 Ac-Arg-Arg-4-methyl-7-amidocoumarin + H2O - Homo sapiens Ac-Arg-Arg + 4-methyl-7-aminocoumarin - ? 411537 3.4.22.1 Ac-Arg-Arg-7-amido-4-trifluoromethylcoumarin + H2O - Homo sapiens ? - ? 449478 3.4.22.1 Ac-Arg-Arg-7-amido-4-trifluoromethylcoumarin + H2O - Rattus norvegicus Ac-Arg-Arg + 7-amino-4-trifluoromethylcoumarin - ? 449479 3.4.22.1 Ac-His-Pro-Val-Lys-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O assay at pH 5.5, 37°C Homo sapiens Ac-His-Pro-Val-Lys + 7-amino-3-carbamoyl-4-methylcoumarin - ? 411538 3.4.22.1 acetyl-Arg-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens acetyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 366702 3.4.22.1 acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O a synthetic substrate of caspase-3 and caspase-7 Rattus norvegicus ? - ? 404188 3.4.22.1 acetyl-DEVD-7-amido-4-methylcoumarin + H2O cleavage occurs at pH 4.0, but not at pH 7.5 Rattus norvegicus acetyl-DEVD + 7-amino-4-methylcoumarin - ? 361962 3.4.22.1 acetyl-F-R-4-methylcoumarin-7-amide + H2O - Homo sapiens acetyl-F-R + 7-amino-4-methylcoumarin - ? 366593 3.4.22.1 acetyl-L-Phe-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens acetyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 366706 3.4.22.1 Albumin + H2O - Naegleria fowleri ? - ? 16041 3.4.22.1 Aldolase + H2O - Rattus norvegicus Hydrolyzed aldolase - ? 15503 3.4.22.1 Aldolase + H2O - Sus scrofa Hydrolyzed aldolase - ? 15503 3.4.22.1 Aldolase + H2O - Bubalus bubalis Hydrolyzed aldolase - ? 15503 3.4.22.1 alpha-N-benzoyl-DL-Arg-beta-naphthylamide + H2O - Capra aegagrus alpha-N-benzoyl-DL-Arg + beta-naphthylamine - ? 366690 3.4.22.1 amino acid-beta-naphthylamides + H2O - Oryctolagus cuniculus amino acid + beta-naphthylamine - ? 36962 3.4.22.1 amyloid beta peptide + H2O - Mus musculus ? - ? 367390 3.4.22.1 amyloid beta peptide + H2O cathepsin B is involved in degradation of amyloid beta peptides, its inhibition can lead to amyloid beta accumulation, an early trigger of Alzheimer's disease, cystatin C regulates the enzyme negatively, CysC ablation ameliorates amyloid beta-associated neuronal and synaptic deficits in hippocampal circuits, neuroprotective effects of CysC ablation depend on CatB, overview Mus musculus ? - ? 367390 3.4.22.1 amyloid beta precursor protein + H2O cathepsin B selectively cleaves the wild-type beta-secretase site but not the rare Swedish mutant beta-secretase site Mus musculus ? - ? 402420 3.4.22.1 amyloid precursor protein C-terminal fragment + H2O cathepsin B degraded C-terminal fragments regardless of phosphorylation Homo sapiens ? - ? 418457 3.4.22.1 amyloid precursor protein intracellular domain + H2O - Homo sapiens ? - ? 418458 3.4.22.1 amyloid-beta + H2O - Mus musculus ? - ? 365809 3.4.22.1 antiprotease secretory leucoprotease inhibitor + H2O i.e. SLP1 protein, cleavage between residues Thr67 and Tyr68 Homo sapiens cleaved SLP1 protein - ? 366623 3.4.22.1 Arg-4-methylcoumaryl-7-amide + H2O - Homo sapiens Arg + 7-amino-4-methylcoumarin - ? 36958 3.4.22.1 Arg-beta-naphthylamide + H2O - Homo sapiens Arg + beta-naphthylamine - ? 36959 3.4.22.1 Arg-beta-naphthylamide + H2O - Capra hircus Arg + beta-naphthylamine - ? 36959 3.4.22.1 azocasein + H2O - Oryctolagus cuniculus fragments of azocasein - ? 15660 3.4.22.1 azocasein + H2O - Tetrahymena pyriformis fragments of azocasein - ? 15660 3.4.22.1 azocasein + H2O poor substrate Macaca fuscata fragments of azocasein - ? 15660 3.4.22.1 benzoyl-arginine ethyl ester + H2O - Homo sapiens benzoyl-arginine + ethanol - ? 36955 3.4.22.1 benzoyl-arginine ethyl ester + H2O - Rattus norvegicus benzoyl-arginine + ethanol - ? 36955 3.4.22.1 benzoyl-arginine ethyl ester + H2O - Sus scrofa benzoyl-arginine + ethanol - ? 36955 3.4.22.1 benzoyl-arginine ethyl ester + H2O - Bos taurus benzoyl-arginine + ethanol - ? 36955 3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O - Mus musculus benzoyl-DL-Arg + beta-naphthylamine - ? 36949 3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O - Homo sapiens benzoyl-DL-Arg + beta-naphthylamine - ? 36949 3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O - Rattus norvegicus benzoyl-DL-Arg + beta-naphthylamine - ? 36949 3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O - Sus scrofa benzoyl-DL-Arg + beta-naphthylamine - ? 36949 3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O - Bos taurus benzoyl-DL-Arg + beta-naphthylamine - ? 36949 3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O - Oryctolagus cuniculus benzoyl-DL-Arg + beta-naphthylamine - ? 36949 3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O - Tetrahymena pyriformis benzoyl-DL-Arg + beta-naphthylamine - ? 36949 3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O - Capra hircus benzoyl-DL-Arg + beta-naphthylamine - ? 36949 3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O - Dorytheuthis bleekeri benzoyl-DL-Arg + beta-naphthylamine - ? 36949 3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O - Bubalus bubalis benzoyl-DL-Arg + beta-naphthylamine - ? 36949 3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O - Gallus gallus benzoyl-DL-Arg + p-nitroaniline - ? 36947 3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O - Homo sapiens benzoyl-DL-Arg + p-nitroaniline - ? 36947 3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O - Rattus norvegicus benzoyl-DL-Arg + p-nitroaniline - ? 36947 3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O - Bos taurus benzoyl-DL-Arg + p-nitroaniline - ? 36947 3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O - Tetrahymena pyriformis benzoyl-DL-Arg + p-nitroaniline - ? 36947 3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O - Capra hircus benzoyl-DL-Arg + p-nitroaniline - ? 36947 3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O - Bubalus bubalis benzoyl-DL-Arg + p-nitroaniline - ? 36947 3.4.22.1 benzoyl-DL-argininamide + H2O - Homo sapiens benzoyl-DL-arginine + NH3 - ? 36954 3.4.22.1 benzoyl-DL-argininamide + H2O - Rattus norvegicus benzoyl-DL-arginine + NH3 - ? 36954 3.4.22.1 benzoyl-DL-argininamide + H2O - Bos taurus benzoyl-DL-arginine + NH3 - ? 36954 3.4.22.1 benzoyl-DL-argininamide + H2O - Oryctolagus cuniculus benzoyl-DL-arginine + NH3 - ? 36954 3.4.22.1 benzoyl-DL-argininamide + H2O - Dorytheuthis bleekeri benzoyl-DL-arginine + NH3 - ? 36954 3.4.22.1 benzoyl-L-3-pyridyl-Ala-l-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens benzoyl-L-3-pyridyl-Ala-L-Arg + 7-amino-4-methylcoumarin - ? 366715 3.4.22.1 benzoyl-L-4-aminocyclohexy-Ala-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens benzoyl-L-4-aminocyclohexyl-Ala-L-Arg + 7-amino-4-methylcoumarin - ? 366717 3.4.22.1 benzoyl-L-4-aminomethyl-N-isopropyl-Phe-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens benzoyl-L-4-aminomethyl-N-isopropyl-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 366714 3.4.22.1 benzoyl-L-4-aminomethyl-Phe-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens benzoyl-L-4-aminomethyl-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 366712 3.4.22.1 benzoyl-L-4-guanidine-Phe-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens benzoyl-L-4-guanidine-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 366713 3.4.22.1 benzoyl-L-4-piperidinyl-Ala-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens benzoyl-L-4-piperidinyl-Ala-L-Arg + 7-amino-4-methylcoumarin - ? 366716 3.4.22.1 benzoyl-L-Arg-beta-naphthylamide + H2O - Capra hircus benzoyl-L-Arg + beta-naphthylamine - ? 36950 3.4.22.1 benzoyl-L-Arg-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens benzoyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 366703 3.4.22.1 benzoyl-L-Arg-p-nitroanilide + H2O - Sus scrofa benzoyl-L-Arg + p-nitroaniline - ? 36948 3.4.22.1 benzoyl-L-Arg-p-nitroanilide + H2O - Bos taurus benzoyl-L-Arg + p-nitroaniline - ? 36948 3.4.22.1 benzoyl-L-Arg-p-nitroanilide + H2O - Capra hircus benzoyl-L-Arg + p-nitroaniline - ? 36948 3.4.22.1 benzoyl-L-His-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens benzoyl-L-His-L-Arg + 7-amino-4-methylcoumarin - ? 366710 3.4.22.1 benzoyl-L-N-dimethyl-His-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens benzoyl-L-N-dimethyl-His-L-Arg + 7-amino-4-methylcoumarin - ? 366711 3.4.22.1 benzoyl-L-Phe-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens benzoyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 366707 3.4.22.1 benzoyl-Phe-Val-Arg-4-methylcoumarin-7-amide + H2O - Oryctolagus cuniculus benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin - ? 36946 3.4.22.1 benzoyl-Phe-Val-Arg-p-nitroanilide + H2O - Mus musculus benzoyl-Phe-Val-Arg + p-nitroaniline - ? 36951 3.4.22.1 benzoyl-Phe-Val-Arg-p-nitroanilide + H2O - Sus scrofa benzoyl-Phe-Val-Arg + p-nitroaniline - ? 36951 3.4.22.1 benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O - Mus musculus benzoyl-Pro-Phe-Arg + p-nitroaniline - ? 36952 3.4.22.1 benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O - Sus scrofa benzoyl-Pro-Phe-Arg + p-nitroaniline - ? 36952 3.4.22.1 benzyloxycarbonyl-Ala-4-nitrophenyl ester + H2O - Homo sapiens benzyloxycarbonyl-Ala + 4-nitrophenol - ? 36944 3.4.22.1 benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide + H2O - Mus musculus benzyloxycarbonyl-Ala-Arg-Arg + 4-methoxy-beta-naphthylamine - ? 36937 3.4.22.1 benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide + H2O - Homo sapiens benzyloxycarbonyl-Ala-Arg-Arg + 4-methoxy-beta-naphthylamine - ? 36937 3.4.22.1 benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide + H2O - Rattus norvegicus benzyloxycarbonyl-Ala-Arg-Arg + 4-methoxy-beta-naphthylamine - ? 36937 3.4.22.1 benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide + H2O - Sus scrofa benzyloxycarbonyl-Ala-Arg-Arg + 4-methoxy-beta-naphthylamine - ? 36937 3.4.22.1 benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide + H2O - Capra hircus benzyloxycarbonyl-Ala-Arg-Arg + 4-methoxy-beta-naphthylamine - ? 36937 3.4.22.1 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide + H2O - Fasciola hepatica benzyloxycarbonyl-Arg + 7-amino-4-methylcoumarin - ? 36934 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide + H2O - Homo sapiens benzyloxycarbonyl-Arg-Arg + 4-methoxy-beta-naphthylamine - ? 36938 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide + H2O - Capra hircus benzyloxycarbonyl-Arg-Arg + 4-methoxy-beta-naphthylamine - ? 36938 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide + H2O - Artemia sp. benzyloxycarbonyl-Arg-Arg + 4-methoxy-beta-naphthylamine - ? 36938 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methyl-7-coumarylamide + H2O - Oreochromis niloticus ? - ? 449648 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Homo sapiens benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Rattus norvegicus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Bos taurus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Oryctolagus cuniculus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Hemicentrotus pulcherrimus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Schistosoma mansoni benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Cyprinus carpio benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Bubalus bubalis benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Heliocidaris crassispina benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Fasciola hepatica benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Scomber australasicus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 36933 3.4.22.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Capra hircus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 362355 3.4.22.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Fundulus heteroclitus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 362355 3.4.22.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Clonorchis sinensis benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 362355 3.4.22.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Rattus norvegicus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 362355 3.4.22.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Nicotiana benthamiana benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 362355 3.4.22.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Fasciola gigantica benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 362355 3.4.22.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O endopeptidase activity Homo sapiens benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 362355 3.4.22.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O 17% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Trachurus japonicus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 362355 3.4.22.1 benzyloxycarbonyl-Arg-Arg-beta-naphthylamide + H2O - Homo sapiens benzyloxycarbonyl-Arg-Arg + beta-naphthylamine - ? 36936 3.4.22.1 benzyloxycarbonyl-Arg-Arg-p-nitroanilide + H2O - Capra hircus benzyloxycarbonyl-Arg-Arg + p-nitroaniline - ? 36941 3.4.22.1 benzyloxycarbonyl-FR-4-methylcoumarin-7-amide + H2O - Homo sapiens benzyloxycarbonyl-FR + 7-amino-4-methylcoumarin - ? 366592 3.4.22.1 benzyloxycarbonyl-Gly-4-nitrophenyl ester + H2O - Homo sapiens benzyloxycarbonyl-Gly + 4-nitrophenol - ? 36945 3.4.22.1 benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens benzyloxycarbonyl-L-Arg + 7-amino-4-methylcoumarin - ? 391447 3.4.22.1 benzyloxycarbonyl-L-Arg-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens benzyloxycarbonyl-L-Arg-Arg + 7-amino-4-methylcoumarin - ? 391448 3.4.22.1 benzyloxycarbonyl-L-Arg-Arg-7-amido-4-methylcoumarin + H2O - Cyprinus carpio benzyloxycarbonyl-L-Arg-Arg + 7-amino-4-methylcoumarin - ? 391448 3.4.22.1 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide + H2O - Naegleria fowleri ? - ? 433500 3.4.22.1 benzyloxycarbonyl-L-Phe-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens benzyloxycarbonyl-L-Phe-Arg + 7-amino-4-methylcoumarin - ? 391453 3.4.22.1 benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 384296 3.4.22.1 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide + H2O - Naegleria fowleri ? - ? 433501 3.4.22.1 benzyloxycarbonyl-Lys-4-nitrophenyl ester + H2O - Homo sapiens benzyloxycarbonyl-Lys + 4-nitrophenol - ? 36943 3.4.22.1 benzyloxycarbonyl-Lys-4-nitrophenyl ester + H2O - Bos taurus benzyloxycarbonyl-Lys + 4-nitrophenol - ? 36943 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methoxy-beta-naphthylamide + H2O - Capra hircus benzyloxycarbonyl-Phe-Arg + 4-methoxy-beta-naphthylamine - ? 36939 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O - Homo sapiens benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 361855 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Gallus gallus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Homo sapiens benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Rattus norvegicus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Sus scrofa benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Bos taurus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Oryctolagus cuniculus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Hemicentrotus pulcherrimus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Schistosoma mansoni benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Cyprinus carpio benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Macaca fuscata benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Bubalus bubalis benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Heliocidaris crassispina benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Fasciola hepatica benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide - Scomber australasicus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 36935 3.4.22.1 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Capra hircus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 361886 3.4.22.1 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Nicotiana benthamiana benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 361886 3.4.22.1 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Trachurus japonicus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 361886 3.4.22.1 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O low activity with cathepsin B3 and B2, higher activity with cathepsin B1 and B4 Clonorchis sinensis benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 361886 3.4.22.1 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Mus musculus benzyloxycarbonyl-L-Phe-Arg + 7-amino-4-methylcoumarin - ? 391460 3.4.22.1 benzyloxycarbonyl-Phe-Arg-AMC + H2O - Homo sapiens ? - ? 449662 3.4.22.1 benzyloxycarbonyl-Phe-Arg-p-nitroanilide + H2O - Capra hircus benzyloxycarbonyl-Phe-Arg + p-nitroaniline - ? 36942 3.4.22.1 benzyloxycarbonyl-phenylalanyl-arginine-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, activity at pH 5.0 is 80fold greater than with Arg-Arg Trichobilharzia regenti Z-Phe-Arg + 7-amino-4-methylcoumarin - ? 384305 3.4.22.1 benzyloxycarbonyl-phenylalanyl-arginyl-7-amido-4-methylcoumarin + H2O 37°C, 1 mM DTT, 1 mM EDTA, pH 6.0 Homo sapiens Z-Phe-Arg + 7-amino-4-methylcoumarin - ? 384306 3.4.22.1 benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide + H2O - Mus musculus ? + 4-methoxy-beta-naphthylamine - ? 36940 3.4.22.1 benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide + H2O - Homo sapiens ? + 4-methoxy-beta-naphthylamine - ? 36940 3.4.22.1 benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide + H2O - Sus scrofa ? + 4-methoxy-beta-naphthylamine - ? 36940 3.4.22.1 benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide + H2O - Capra hircus ? + 4-methoxy-beta-naphthylamine - ? 36940 3.4.22.1 beta-lipotropin + H2O - Bos taurus hydrolyzed beta-lipotropin - ? 36979 3.4.22.1 Boc-Asp-Pro-Arg-4-methyl-7-amidocoumarin + H2O - Homo sapiens Boc-Asp-Pro-Arg + 4-methyl-7-aminocoumarin - ? 411814 3.4.22.1 Boc-Asp-Pro-Arg-4-methylcoumarin 7-amide + H2O - Fasciola hepatica Boc-Asp-Pro-Arg + 7-amino-4-methylcoumarin - ? 411815 3.4.22.1 Boc-L-Leu-L-Lys-L-Arg-4-methylcoumarin-7-amide + H2O pH-dependence of reaction Homo sapiens Boc-L-Leu-L-Lys-L-Arg + 7-amino-4-methylcoumarin - ? 366589 3.4.22.1 Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O 29% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Trachurus japonicus Boc-Phe-Ser-Arg + 7-amino-4-methylcoumarin - ? 428451 3.4.22.1 Boc-Val-Leu-Lys-4-methyl-7-amidocoumarin + H2O - Homo sapiens Boc-Val-Leu-Lys + 4-methyl-7-aminocoumarin - ? 411818 3.4.22.1 Boc-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O - Fasciola hepatica Boc-Val-Pro-Arg + 7-amino-4-methylcoumarin - ? 366223 3.4.22.1 Boc-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O 8% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Trachurus japonicus Boc-Val-Pro-Arg + 7-amino-4-methylcoumarin - ? 449704 3.4.22.1 BODIPY FL casein + H2O - Homo sapiens ? - ? 411821 3.4.22.1 Bovine serum albumin + H2O - Artemia sp. Hydrolyzed bovine serum albumin - ? 15513 3.4.22.1 Bovine serum albumin + H2O - Bubalus bubalis Hydrolyzed bovine serum albumin - ? 15513 3.4.22.1 Bovine serum albumin + H2O - Fasciola hepatica Hydrolyzed bovine serum albumin - ? 15513 3.4.22.1 carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin - ? 404592 3.4.22.1 carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O - Rattus norvegicus carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin - ? 404592 3.4.22.1 carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O - Bos taurus carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin - ? 404592 3.4.22.1 carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O a fluorogenic substrate of Na-CP-3 Homo sapiens carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin - ? 404592 3.4.22.1 carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O an endoproteolytic substrate Homo sapiens carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin - ? 404592 3.4.22.1 carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin hydrochloride + H2O - Homo sapiens carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin hydrochloride - ? 404593 3.4.22.1 carbobenzoxy-Gly-Gly-Arg-naphthylamide + H2O - Oryctolagus cuniculus ? + naphthylamine - ? 36956 3.4.22.1 carbobenzoxy-Phe-Ala-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens carbobenzoxy-Phe-Ala-Arg + 7-amino-4-methylcoumarin - ? 404595 3.4.22.1 carbobenzoxy-Phe-Ala-Arg-7-amido-4-methylcoumarin + H2O a fluorogenic substrate Homo sapiens carbobenzoxy-Phe-Ala-Arg + 7-amino-4-methylcoumarin - ? 404595 3.4.22.1 carbobenzoxy-Phe-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens carbobenzoxy-Phe-Arg + 7-amino-4-methylcoumarin - ? 404596 3.4.22.1 carbobenzoxy-Phe-Arg-7-amido-4-methylcoumarin + H2O a fluorogenic substrate Homo sapiens carbobenzoxy-Phe-Arg + 7-amino-4-methylcoumarin - ? 404596 3.4.22.1 carbobenzoxy-Phe-Arg-7-amido-4-methylcoumarin + H2O a fluorogenic substrate Biomphalaria glabrata carbobenzoxy-Phe-Arg + 7-amino-4-methylcoumarin - ? 404596 3.4.22.1 carbobenzoxy-Phe-Arg-7-amido-4-methylcoumarin + H2O a fluorogenic substrate of Na-CP-3 Necator americanus carbobenzoxy-Phe-Arg + 7-amino-4-methylcoumarin - ? 404596 3.4.22.1 casein + H2O - Capra hircus hydrolyzed casein - ? 15305 3.4.22.1 casein + H2O - Bubalus bubalis hydrolyzed casein - ? 15305 3.4.22.1 caspase 1 precursor + H2O - Homo sapiens ? - ? 417910 3.4.22.1 CBZ-Arg-Arg-4-nitroanilide + H2O - Homo sapiens CBZ-Arg-Arg + 4-nitroaniline - ? 433542 3.4.22.1 CBZ-Arg-Arg-7-amido-4-methylcoumarin + H2O endopeptidase activity Homo sapiens CBZ-Arg-Arg + 7-amino-4-methylcoumarin - ? 433543 3.4.22.1 Cbz-FR-7-amido-4-methylcoumarin + H2O 37°C, pH 6.0, 200 mM NaCl, 1 mM EDTA, 5 nM DTT Homo sapiens Cbz-Phe-Arg + 7-amino-4-methylcoumarin - ? 384417 3.4.22.1 CBZ-L-Arg-L-Arg-4-nitroanilide + H2O - Scophthalmus maximus L-Arg-L-Arg + 4-nitroaniline - ? 433545 3.4.22.1 Cbz-L-Arg-L-Arg-7-amido-4-trifluoromethylcoumarin + H2O - Homo sapiens Cbz-L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 418539 3.4.22.1 CBZ-Phe-Arg-7-amido-4-methylcoumarin + H2O - Fasciola gigantica ? - ? 384418 3.4.22.1 chemokine (C-C motif) ligand 20 + H2O CCL20 is also known as liver- and activation-regulated chemokine (LARC), MIP-3alpha, or exodus-1 Homo sapiens CCL20 1-66 isoform + ? cathepsin B specifically cleaves off four C-terminally located amino acids and generates a CCL20 1-66 isoform with full functional activity ? 391616 3.4.22.1 Collagen + H2O - Homo sapiens Hydrolyzed collagen - ? 15526 3.4.22.1 Collagen + H2O - Sus scrofa Hydrolyzed collagen - ? 15526 3.4.22.1 Collagen + H2O - Bos taurus Hydrolyzed collagen - ? 15526 3.4.22.1 Collagen + H2O - Oryctolagus cuniculus Hydrolyzed collagen - ? 15526 3.4.22.1 Collagen + H2O - Homo sapiens ? - ? 16347 3.4.22.1 Collagen + H2O - Oryctolagus cuniculus ? - ? 16347 3.4.22.1 Collagen + H2O - Naegleria fowleri ? - ? 16347 3.4.22.1 Collagen IV + H2O - Mus musculus ? - ? 17094 3.4.22.1 Collagen IV + H2O - Homo sapiens ? - ? 17094 3.4.22.1 Collagen IV + H2O collagen IV of the basement membrane damaged by cathepsin B, that is released after mechanical injury, during initial post-traumatic stages, overview Mus musculus ? - ? 17094 3.4.22.1 Collagen IV + H2O quenched fluorescent protein Homo sapiens ? - ? 17094 3.4.22.1 D-Pro-Phe-Arg-p-nitroanilide + H2O - Artemia sp. D-Pro-Phe-Arg + p-nitroaniline - ? 36960 3.4.22.1 D-Val-Leu-Arg-4-methylcoumarin 7-amide + H2O - Fasciola hepatica D-Val-Leu-Arg + 7-amino-4-methylcoumarin - ? 411955 3.4.22.1 D-Val-Leu-Lys-p-nitroanilide + H2O - Artemia sp. D-Val-Leu-Lys + p-nitroaniline - ? 36961 3.4.22.1 denatured hemoglobin + H2O - Homo sapiens hydrolyzed denatured hemoglobin - ? 36970 3.4.22.1 denatured hemoglobin + H2O - Tetrahymena pyriformis hydrolyzed denatured hemoglobin - ? 36970 3.4.22.1 denatured hemoglobin + H2O - Bubalus bubalis hydrolyzed denatured hemoglobin - ? 36970 3.4.22.1 Dnp-Gly-Phe-Arg-Trp-X-OH + H2O kcat/Km for Dnp-Gly-Phe-Arg-Trp-X-OH, with X is Phe > Tyr, Ile > Leu > Cval > Arg > Lys, Pro > Gln, Ser > Asp, Glu, Ala Homo sapiens Dnp-Gly-Phe + Arg-Trp-X-OH - ? 449874 3.4.22.1 Dnp-Gly-Phe-Arg-X-Trp-OH + H2O kcat/Km for Dnp-Gly-Phe-Arg-X-Trp-OH, with X is Phe > Ser > Leu > Ala > Ile > Tyr, Val > Gln > Lys > Gly > His, Asp > Arg > Glu Homo sapiens Dnp-Gly-Phe + Arg-X-Trp-OH - ? 449875 3.4.22.1 Dnp-Gly-Phe-X-Phe-Trp-OH + H2O kcat/Km for Dnp-Gly-Phe-X-Phe-Trp-OH, with X is Arg > Lys > Phe > Leu > Val > His, Gln > Ser > Gly Ala > Glu > Tyr > Ile, Asp Homo sapiens Dnp-Gly-Phe-X + Phe-Trp-OH - ? 449876 3.4.22.1 Dnp-Gly-X-Arg-Phe-Trp-OH + H2O kcat/Km for Dnp-Gly-X-Arg-Phe-Trp-OH, with X is Phe > Tyr > Lys > Val > Ala > Ile > Ser, Pro > His > Leu > Arg > Asp, Glu Homo sapiens Dnp-Gly-X-Arg + Phe-Trp-OH - ? 449877 3.4.22.1 DQ-collagen IV + H2O - Homo sapiens ? - ? 412011 3.4.22.1 epithelial sodium channel alpha subunit + H2O in vitro cleavage studies show that the full-length ENaC alpha subunit fusion protein is cleaved by active cathepsin B but not the full-length beta or gamma subunit fusion proteins Xenopus laevis ? - ? 433707 3.4.22.1 epsilon-aminocaproic acid-L-Leu-Gly-4-methylcoumarin-7-amide + H2O - Homo sapiens epsilon-aminocaproic acid-L-Leu-Gly + 7-amino-4-methylcoumarin - ? 366599 3.4.22.1 epsilon-aminocaproic acid-L-Leu-L-(O-benzyl)serine-4-methylcoumarin-7-amide + H2O - Homo sapiens epsilon-aminocaproic acid-L-Leu-L-(O-benzyl)serine + 7-amino-4-methylcoumarin - ? 366597 3.4.22.1 epsilon-aminocaproic acid-L-Leu-L-(O-benzyl)threonine-4-methylcoumarin-7-amide + H2O - Homo sapiens epsilon-aminocaproic acid-L-Leu-L-(O-benzyl)threonine + 7-amino-4-methylcoumarin - ? 366596 3.4.22.1 epsilon-aminocaproic acid-L-Leu-L-(O-methyl)-tyrosine-4-methylcoumarin-7-amide + H2O - Homo sapiens epsilon-aminocaproic acid-L-Leu-L-(O-methyl)-tyrosine + 7-amino-4-methylcoumarin - ? 366598 3.4.22.1 epsilon-aminocaproic acid-L-Leu-L-(S-benzyl)cysteine-4-methylcoumarin-7-amide + H2O - Homo sapiens epsilon-aminocaproic acid-L-Leu-L-(S-benzyl)cysteine + 7-amino-4-methylcoumarin - ? 366595 3.4.22.1 epsilon-aminocaproic acid-L-Leu-L-Phe-4-methylcoumarin-7-amide + H2O - Homo sapiens epsilon-aminocaproic acid-L-Leu-L-Phe + 7-amino-4-methylcoumarin - ? 366600 3.4.22.1 epsilon-aminocaproic acid-L-R-4-methylcoumarin-7-amide + H2O - Homo sapiens epsilon-aminocaproic acid-L-R + 7-amino-4-methylcoumarin - ? 366594 3.4.22.1 Fibronectin + H2O - Naegleria fowleri ? - ? 15394 3.4.22.1 filaggrin + H2O - Rattus norvegicus hydrolyzed filaggrin - ? 36984 3.4.22.1 Gelatin + H2O - Mus musculus ? - ? 16570 3.4.22.1 Gelatin + H2O - Homo sapiens ? - ? 16570 3.4.22.1 Gelatin + H2O - Ancylostoma caninum ? - ? 16570 3.4.22.1 Gelatin + H2O - Fasciola gigantica ? - ? 16570 3.4.22.1 Gelatin + H2O a substrate of Na-CP-3 Necator americanus ? - ? 16570 3.4.22.1 Gelatin + H2O quenched fluorescent protein Homo sapiens ? - ? 16570 3.4.22.1 gelatin + H2O - Oryctolagus cuniculus hydrolyzed gelatine - ? 36965 3.4.22.1 globin + H2O - Schistosoma mansoni partially degraded globin - ? 36982 3.4.22.1 Glucagon + H2O - Sus scrofa Hydrolyzed glucagon - ? 15306 3.4.22.1 Gly-Arg-7-amido-4-methylcoumarin + H2O a fluorogenic substrate Homo sapiens Gly-Arg + 7-amino-4-methylcoumarin - ? 381971 3.4.22.1 Hemoglobin + H2O - Capra hircus ? - ? 15450 3.4.22.1 Hemoglobin + H2O - Naegleria fowleri ? - ? 15450 3.4.22.1 Hemoglobin + H2O from goat and from buffalo Bubalus bubalis Hydrolyzed hemoglobin - ? 15477 3.4.22.1 herpes simplex virus type 1 origin binding protein + H2O 53 kDa protein Homo sapiens herpes simplex virus type 1 origin binding protein 1 50 kDa protein ? 392199 3.4.22.1 histones + H2O - Bos taurus hydrolyzed histones - ? 36980 3.4.22.1 human anti-HIV-1 antibody + H2O NbCathB is far more efficient than aleurain-like protease in processing the human anti-HIV-1 antibody 2F5 into fragments Nicotiana benthamiana ? - ? 450053 3.4.22.1 human fibronectin + H2O - Clonorchis sinensis ? - ? 382039 3.4.22.1 human hemoglobin + H2O - Clonorchis sinensis ? - ? 365218 3.4.22.1 human IgG + H2O - Clonorchis sinensis ? - ? 189962 3.4.22.1 human serum albumin + H2O - Clonorchis sinensis ? - ? 431959 3.4.22.1 IgA + H2O - Naegleria fowleri ? - ? 366585 3.4.22.1 IgG + H2O - Fasciola gigantica ? - ? 366194 3.4.22.1 IgG + H2O - Naegleria fowleri ? - ? 366194 3.4.22.1 L-Arg-L-Arg-4-nitroanilide + H2O - Cynoglossus semilaevis L-Arg-L-Arg + 4-nitroaniline - ? 418747 3.4.22.1 L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 418748 3.4.22.1 L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O - Penaeus vannamei L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 418748 3.4.22.1 L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 418771 3.4.22.1 Laminin + H2O - Mus musculus ? - ? 16571 3.4.22.1 Laminin + H2O laminin of the basement membrane damaged by cathepsin B, that is released after mechanical injury, during initial post-traumatic stages, overview Mus musculus ? - ? 16571 3.4.22.1 additional information enzyme prefers dipeptidyl pathway over monopeptidyl carboxypeptidase pathway Homo sapiens ? - ? 89 3.4.22.1 additional information enzyme prefers large aromatic residues at S2' subsite and the exopeptidase activity prefers aromatic side chains in P2' position Homo sapiens ? - ? 89 3.4.22.1 additional information preference for substrate cleavage through dipeptidyl pathway, influence of substrate P2-P1 residues Homo sapiens ? - ? 89 3.4.22.1 additional information enzyme is involved in myoblast-myoblast fusion Rattus norvegicus ? - ? 89 3.4.22.1 additional information cathepsin B can partially process the 48000 Da intermediate into 34000 Da mature cathepsin D Homo sapiens ? - ? 89 3.4.22.1 additional information no degradation of hemoglobin Trichobilharzia regenti ? - ? 89 3.4.22.1 additional information no reaction with Abz-GIVRPK(Dnp)-OH Homo sapiens ? - ? 89 3.4.22.1 additional information no reaction with procarboxypeptidase and proelastase Homo sapiens ? - ? 89 3.4.22.1 additional information this isoform encodes an inactive enzyme Trichobilharzia regenti ? - ? 89 3.4.22.1 additional information cathepsin B is able to self-activate Homo sapiens ? - ? 89 3.4.22.1 additional information CmCatB2 may be an inactive protease isoform resulting from splicing errors Callosobruchus maculatus ? - ? 89 3.4.22.1 additional information absence of Ctsb significantly impairs development of high-grade invasive ductal carcinomas and reduces the metastatic burden in the lungs, and cancer cells lacking Ctsb exhibit significantly higher resistance to apoptosis induction by the lysosomotropic agent Leu-Leu-OMe Mus musculus ? - ? 89 3.4.22.1 additional information autocatalytic activation of the inactive proenzyme Homo sapiens ? - ? 89 3.4.22.1 additional information cathepsin B belongs to the lysosomal cysteine cathepsins, a third major class of matrix-degrading enzymes involved in tumor invasion and tissue remodeling. Cathepsin B is involved in matrix degradation at podosomes, cysteine protease inhibitors reduce matrix degradation and invasion in vitro Mus musculus ? - ? 89 3.4.22.1 additional information cathepsin B is a lysosomal cysteine protease of the papain-like enzyme family with multiple biological functions Paralichthys olivaceus ? - ? 89 3.4.22.1 additional information cathepsin B is a target of Hedgehog signaling in pancreatic cancer, downregulation of CATB by Hedgehog, cyclopamine reduces CATB protein and mRNA levels. CATB might influence pancreatic cancer cell invasiveness Homo sapiens ? - ? 89 3.4.22.1 additional information cathepsin B is an intracellular lysosomal cysteine proteinase that can degrade extracellular components including collagen and protein turnover in the lysosomal system. Cathepsin B plays an important role in the resolution of organic matrix, a final step in bone resorption. It is also known to play an important role in the initiation and perpetuation of inflammatory processes Homo sapiens ? - ? 89 3.4.22.1 additional information cathepsin B is expressed in cerebral aneurysms and promotes the progression of cerebral aneurysms, which include degradation of extracellular matrix in arterial walls in strong subarachnoid hemorrhage, overview Homo sapiens ? - ? 89 3.4.22.1 additional information cathepsin B is expressed in induced cerebral aneurysms and promotes the progression of cerebral aneurysms, which include degradation of extracellular matrix in arterial walls in strong subarachnoid hemorrhage, overview Rattus norvegicus ? - ? 89 3.4.22.1 additional information cathepsin B is involved in the trafficking of TNF-alpha-containing vesicles to the plasma membrane in macrophages Mus musculus ? - ? 89 3.4.22.1 additional information cathepsin B is involved in the trafficking of TNF-alpha-containing vesicles to the plasma membrane in macrophages Homo sapiens ? - ? 89 3.4.22.1 additional information cathepsin B plays a key role in parasite infection, overview Trypanosoma brucei ? - ? 89 3.4.22.1 additional information cathepsin B plays an essential role in the pathogenesis of fulminant hepatic failure, and the cathepsin B inhibitor CA-074me can attenuate apoptosis and liver injury, overview Mus musculus ? - ? 89 3.4.22.1 additional information cathepsin B secreted by activated microglia is closely associated with the MeHg-induced severe pyknotic changes of the cerebellar granule cells, overview. Cathepsin B is involved in apoptotic processes Rattus norvegicus ? - ? 89 3.4.22.1 additional information cathepsin B, together with cathepsin L, urokinase-type plasminogen activator and its inhibitor PAI-1, plays an important role in colorectal cancer invasion. Correlation analysis of proteolytic enzymes in colorectal cancer, overview Homo sapiens ? - ? 89 3.4.22.1 additional information cathepsin-B is a lysosomal cysteine proteasewith broad substrate specificity, which belongs to a family of 11 cysteine proteases. The enzyme is involved in tumor progression, e.g. of endometrial carcinomas Homo sapiens ? - ? 89 3.4.22.1 additional information cysteine cathepsins play a critical role in the biologic activity of tumor-shed vesicles at acidic pH, but not at physiological pH, endothelial cell invasiveness is increased by tumor-shed vesicles exposed to acidic pH, overview Homo sapiens ? - ? 89 3.4.22.1 additional information differential regulation of snail cathepsin B transcript pre- and postparasite exposure, overview Biomphalaria glabrata ? - ? 89 3.4.22.1 additional information disturbances in the regulation of the intracellular activities and uncontrolled release of cathepsins from lysosomes have been implicated in many disease states, such as osteoporosis, inflammatory diseases, and tumor progression Homo sapiens ? - ? 89 3.4.22.1 additional information effective inhibition of cathepsin B can decrease the severity of joint inflammation and to reduce the destruction of particular tissues in the rat model of antigen adjuvant-induced arthritis Rattus norvegicus ? - ? 89 3.4.22.1 additional information increased cathepsin B levels in acute and chronic lung diseases resulting from high levels of extracellular neutrophil elastase, expression of the protease can be inhibited by alpha1-antitrypsin augmentation therapy Homo sapiens ? - ? 89 3.4.22.1 additional information inhibitors of cathepsin B improve memory and reduce beta-amyloid in transgenic Alzheimer disease mice expressing the wild-type, but not the Swedish mutant, beta-secretase site of the amyloid precursor protein Mus musculus ? - ? 89 3.4.22.1 additional information NF-kappaB induced up-regulation of cathepsin B expression contributes to the invasive property of the 143B cells lacking mtDNA, overview. Extracellularly released cathepsin B can act alone or in concert with some matrix metalloproteinases Homo sapiens ? - ? 89 3.4.22.1 additional information participation of cathepsin B in emodin-induced apoptosis in HK-2 cells, enzyme inhibition by Ca-074 causes significant attenuation the ratio of hypodiploid and apoptotic cells, partially blockage of caspase 3 activation and inhibition of reduction of cell viability induced by emodin, overview Homo sapiens ? - ? 89 3.4.22.1 additional information Porphyromonas gingivalis strain 381 or its lipopolysaccharides increases the expression of cathepsin B in oral epithelial cells Homo sapiens ? - ? 89 3.4.22.1 additional information regulation of cathepsin B in gingival fibroblasts involves interleukin-6 and its receptor along with the Cav-1-JNK-AP-1 pathway, overview Homo sapiens ? - ? 89 3.4.22.1 additional information the enzyme has a principle function in gross protein degradation of internalized proteins within endo-lysosomes, but cathepsins are also involved in the processing of precursor proteins to biologically active peptides in endosomes of entero-endocrine cells. Cathepsin B, released form intestinal mucosa due to mechanical injury, plays an important role in extracellular matrix damage and the onset of postoperative ileus, an inevitable consequence of abdominal surgery, in that it contributes to disintegration of the basement membrane of the gastrointestinal tract in early post-traumatic phases. Cathepsin B is best known for its critical contribution to enhanced tumor cell invasion and metastasis, including colorectal carcinomas, by degradation of extracellular matrix components Mus musculus ? - ? 89 3.4.22.1 additional information the enzyme is involved in degradation of extracellular matrix and of basement membrane components leading to tumor invasion in the intestine. Expression of matrix metalloproteinase-9 and cathepsin B is correlated with lymph node metastasis in advanced gastric carcinoma, but not with patients' postoperative survival, overview. Correlation between the synthesis of cysteine and serine proteases and the potential tumor invasion Homo sapiens ? - ? 89 3.4.22.1 additional information the enzyme is involved in extracellular matrix degradation in caveolae of endothelial cells during tube formation, overview. Cathepsin B regulates both pro- and anti-angiogenic factors and may be a contributor to the angiogenic switch of endothelial cells Homo sapiens ? - ? 89 3.4.22.1 additional information the enzyme is involved in inflammatory processes Homo sapiens ? - ? 89 3.4.22.1 additional information the enzyme plays an important role in cancer invasion and metastasis, the enzyme content is positively correlated with tumors in different tissue types Homo sapiens ? - ? 89 3.4.22.1 additional information under certain conditions, e.g. in hypoxia, cathepsin B participates in cleavage of caspase-3 substrates in brain cells Rattus norvegicus ? - ? 89 3.4.22.1 additional information cathepsin B is a lysosomal cysteine protease, a unique cysteine member showing dual roles as both endopeptidase and exopeptidase activities due to the presence of the occluding loop in structure Rattus norvegicus ? - ? 89 3.4.22.1 additional information no activity with carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin by Na-CP-3, recombinant Na-CP-3 does not digest intact hemoglobin but digests globin fragments generated by prior hydrolysis with aspartic hemoglobinases Necator americanus ? - ? 89 3.4.22.1 additional information autocatalytic activation of procathepsin B is largely insensitive to mutations in the cleavage site region and can proceed at neutral pH when bound to heparin and other negatively charged surfaces, which may account for an extracellular physiological role of cathepsins Homo sapiens ? - ? 89 3.4.22.1 additional information Boc-Val-Leu-Lys-4-methylcoumarin 7-amide is no substrate of catB1. CatB1 substrate specificity, homology modelling and molecular dynamics simulations of FhcatB1 interaction with peptide substrates, molecular modelling, overview. A P2 Ile residue positions the substrate optimally for interaction with catalytic residues of the enzyme, and the enzyme lacks an occluding loop His residue crucial for exopeptidase activity. The enzyme performs autolytic processing Fasciola hepatica ? - ? 89 3.4.22.1 additional information cathepsin B is a cysteine protease with both endopeptidase and exopeptidase activity Homo sapiens ? - ? 89 3.4.22.1 additional information No activity with Boc-Val-Pro-Arg-4-methyl-7-amidocoumarin, Boc-Val-Leu-Lys-4-methyl-7-amidocoumarin, and D-Val-Leu-Arg-4-methyl-7-amidocoumarin. Wild-type cathepsin B exhibits exopeptidase activity Homo sapiens ? - ? 89 3.4.22.1 additional information procathepsin B shows autocatalytic processing triggered by proenzyme activity, identification of cleavage sites and initiation mechanism, overview. A multi-step process, starting with a unimolecular conformational change of the zymogen, which unmasks the active site and, in the presence of negatively charged molecules and/or surfaces, also converts the zymogen into a better substrate, followed by the bimolecular proteolytic removal of the propeptide Homo sapiens ? - ? 89 3.4.22.1 additional information the assay uses a modified aminomethylcoumarin dipeptide substrate, which generates a fluorescent signal upon cleavage by cathepsin B Homo sapiens ? - ? 89 3.4.22.1 additional information no activity with benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin Hysterothylacium aduncum ? - ? 89 3.4.22.1 additional information co-incubation of recombinant LycCatB with recombinant Lyc-TR-Ii (invariant chain) leads to an efficient cleavage of recombinant Lyc-TR-Ii Larimichthys crocea ? - ? 89 3.4.22.1 additional information active site mapping with peptidic substrates and inhibitors Homo sapiens ? - ? 89 3.4.22.1 additional information no activity with Arg-7-amido-4-methylcoumarin Trachurus japonicus ? - ? 89 3.4.22.1 additional information the enzyme shows substantial exopeptidase activity at a relative rate of 34.26% compared to human cathepsin B Fasciola gigantica ? - ? 89 3.4.22.1 additional information cathepsin B is involved in the trafficking of TNF-alpha-containing vesicles to the plasma membrane in macrophages Mus musculus C57BL/6 ? - ? 89 3.4.22.1 additional information cathepsin B plays a key role in parasite infection, overview Trypanosoma brucei 90-13 ? - ? 89 3.4.22.1 mouse T7 trypsinogen mutant D22A/K24G + H2O engineering of a mouse T7 trypsinogen mutant (D22A,K24G), which is robustly activated by cathepsin B but cannot undergo autoactivation Mus musculus ? - ? 450301 3.4.22.1 myelin basic protein + H2O - Trichobilharzia regenti ? - ? 140925 3.4.22.1 myoglobin + H2O - Homo sapiens hydrolyzed myoglobin - ? 36972 3.4.22.1 myosin + H2O - Macaca fuscata hydrolyzed myosin - ? 36968 3.4.22.1 myristoylated alanine-rich C kinase substrate + H2O i.e. MARCKS Bos taurus p40 + ? - ? 36981 3.4.22.1 myristoylated alanine-rich C kinase substrate + H2O i.e. MARCKS Bos taurus ? - ? 369624 3.4.22.1 N,N'-diBoc-dityrosine-Gly-(isoniazid)2 + H2O - Homo sapiens ? - ? 433956 3.4.22.1 N,N'-diBoc-dityrosine-Lys-(isoniazid)2 + H2O - Homo sapiens ? - ? 433957 3.4.22.1 N,N-dimethyl hemoglobin + H2O - Dorytheuthis bleekeri hydrolyzed N,N-dimethyl hemoglobin - ? 36973 3.4.22.1 N-alpha-benzyloxycarbonyl-L-arginyl-L-arginine-4-methyl-7-coumarylamide + H2O - Homo sapiens N-alpha-benzyloxycarbonyl-L-arginyl-L-arginine + 4-methyl-7-coumarylamine - ? 405964 3.4.22.1 N-benzoyl-Arg-Arg-4-nitroanilide + H2O - Callosobruchus maculatus N-benzoyl-Arg-Arg + 4-nitroaniline - ? 405967 3.4.22.1 N-benzoyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Mus musculus N-benzoyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 405968 3.4.22.1 N-benzoyl-L-Arg-2-naphthylamide + H2O - Capra hircus N-benzoyl-L-Arg + 2-naphthylamine - ? 433986 3.4.22.1 N-benzoyl-Val-Asn-Leu-7-amido-4-methylcoumarin + H2O the substrate represents the wild-type beta-secretase site Mus musculus N-benzoyl-Val-Lys-Met + 7-amino-4-methylcoumarin - ? 405971 3.4.22.1 N-benzoyl-Val-Lys-Met-7-amido-4-methylcoumarin + H2O the substrate represents the wild-type beta-secretase site Mus musculus N-benzoyl-Val-Lys-Met + 7-amino-4-methylcoumarin - ? 405972 3.4.22.1 N-benzyloxycarbonyl-Arg-Arg-4-methyl-7-amidocoumarin + H2O - Homo sapiens N-benzyloxycarbonyl-Arg-Arg + 4-methyl-7-aminocoumarin - ? 412512 3.4.22.1 N-benzyloxycarbonyl-Arg-Arg-4-methyl-7-amidocoumarin + H2O - Danio rerio N-benzyloxycarbonyl-Arg-Arg + 4-methyl-7-aminocoumarin - ? 412512 3.4.22.1 N-benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Mus musculus N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 412513 3.4.22.1 N-benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Homo sapiens N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 412513 3.4.22.1 N-benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Fasciola hepatica N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 412513 3.4.22.1 N-benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O - Mus musculus BALB/c N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 412513 3.4.22.1 N-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Mus musculus N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 355603 3.4.22.1 N-benzyloxycarbonyl-L-Arg-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens N-benzyloxycarbonyl-L-Arg-Arg + 7-amino-4-methylcoumarin - ? 392759 3.4.22.1 N-benzyloxycarbonyl-L-Arg-Arg-7-amido-4-methylcoumarin + H2O - Nicotiana benthamiana N-benzyloxycarbonyl-L-Arg-Arg + 7-amino-4-methylcoumarin - ? 392759 3.4.22.1 N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 392774 3.4.22.1 N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O - Angiostrongylus cantonensis N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 392774 3.4.22.1 N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O - Apostichopus japonicus N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 392774 3.4.22.1 N-benzyloxycarbonyl-L-arginyl-L-arginyl-4-methylcoumarin 7-amide + H2O substrate of mature cathepsin B, also procathepsin B is catalytically active on the synthetic substrate but to a lower extent. The unfolded proenzymeis inactive Homo sapiens N-benzyloxycarbonyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin - ? 412515 3.4.22.1 N-benzyloxycarbonyl-L-Leu-L-Arg 7-amido-4-methylcoumarin + H2O - Homo sapiens benzyloxycarbonyl-L-Leu-L-Arg + 7-amino-4-methylcoumarin - ? 391450 3.4.22.1 N-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester + H2O - Homo sapiens ? - ? 412516 3.4.22.1 N-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester + H2O - Bos taurus ? - ? 412516 3.4.22.1 N-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester + H2O - Bos taurus N-benzyloxycarbonyl-L-lysine + 4-nitrophenol - ? 412517 3.4.22.1 N-benzyloxycarbonyl-L-Val-Lys-Lys-Arg-beta-naphthylamide + H2O - Homo sapiens N-benzyloxycarbonyl-L-Val-Lys-Lys-Arg + beta-naphthylamine - ? 392777 3.4.22.1 N-benzyloxycarbonyl-Phe-Arg-4-methyl-7-amidocoumarin + H2O - Homo sapiens N-benzyloxycarbonyl-Phe-Arg + 4-methyl-7-aminocoumarin - ? 412519 3.4.22.1 N-benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O - Mus musculus N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 412520 3.4.22.1 N-benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O - Homo sapiens N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 412520 3.4.22.1 N-benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O - Fasciola hepatica N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 412520 3.4.22.1 N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Mus musculus N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 355600 3.4.22.1 N-benzyloxycarbonyl-Val-Ile-Arg-4-methyl-7-amidocoumarin + H2O - Homo sapiens N-benzyloxycarbonyl-Val-Ile-Arg + 4-methyl-7-aminocoumarin - ? 412522 3.4.22.1 N-benzyloxycarbonyl-Val-Ile-Arg-4-methylcoumarin 7-amide + H2O - Fasciola hepatica N-benzyloxycarbonyl-Val-Ile-Arg + 7-amino-4-methylcoumarin - ? 412523 3.4.22.1 N-carbobenzoxy-L-lysine 4-nitrophenyl ester + H2O - Homo sapiens N-carbobenzoxy-L-Lys + 4-nitrophenol - ? 412533 3.4.22.1 N-carbobenzoxy-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O - Apostichopus japonicus N-carbobenzoxy-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 418886 3.4.22.1 N-CBZ-L-Arg-L-Arg-7-amido- 4-methylcoumarin + H2O - Homo sapiens N-CBZ-L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 434000 3.4.22.1 N-t-butyloxycarbonyl-Gln-p-nitrophenyl ester + H2O - Sus scrofa N-t-butyloxycarbonyl-Gln + 4-nitrophenol - ? 36957 3.4.22.1 Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens Nalpha-benzoyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 406093 3.4.22.1 Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Mus musculus Nalpha-benzoyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 406093 3.4.22.1 Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens Nalpha-benzoyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 406095 3.4.22.1 Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Mus musculus Nalpha-benzoyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 406095 3.4.22.1 Nalpha-benzoyl-Val-Lys-Lys-Arg-7-amido-4-trifluoromethylcoumarin + H2O - Homo sapiens ? - ? 406096 3.4.22.1 Nalpha-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O - Hysterothylacium aduncum Nalpha-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 418913 3.4.22.1 Nalpha-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-trifluoromethylcoumarin + H2O - Meretrix meretrix Nalpha-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-trifluoromethylcoumarin - ? 418934 3.4.22.1 Nalpha-CBZ-Arg-Arg 7-amido-4-methylcoumarin + H2O 1 mM DTT Homo sapiens Nalpha-CBZ-Arg-Arg + 7-amino-4-methylcoumarin - ? 385385 3.4.22.1 neuropeptides + H2O such as Leu-enkephalin, beta-neoendorphin, alpha-neoendorphin, dynorphin(1-13), substance P Macaca fuscata hydrolyzed neuropeptides - ? 36969 3.4.22.1 o-aminobenzoic acid-L-Lys-L-Leu-Gly-L-Phe-L-Ser-L-Lys-L-Gln-2,4-dinitrophenyl-ethylenediamine + H2O - Homo sapiens o-aminobenzoic acid-L-Lys-L-Leu-Gly-L-Phe-L-Ser-L-Lys-L-Gln + 2,4-dinitrophenyl-ethylenediamine - ? 366604 3.4.22.1 o-aminobenzoic acid-L-Lys-L-Leu-L-(O-benzyl)serine-L-Phe-L-Ser-L-Lys-L-Gln-2,4-dinitrophenyl-ethylenediamine + H2O - Homo sapiens o-aminobenzoic acid-L-Lys-L-Leu-L-(O-benzyl)serine-L-Phe-L-Ser-L-Lys-L-Gln + 2,4-dinitrophenyl-ethylenediamine - ? 366603 3.4.22.1 o-aminobenzoic acid-L-Lys-L-Leu-L-(O-benzyl)threonine-L-Phe-L-Ser-L-Lys-L-Gln-2,4-dinitrophenyl-ethylenediamine + H2O - Homo sapiens o-aminobenzoic acid-L-Lys-L-Leu-L-(O-benzyl)threonine-L-Phe-L-Ser-L-Lys-L-Gln + 2,4-dinitrophenyl-ethylenediamine - ? 366602 3.4.22.1 o-aminobenzoic acid-L-Lys-L-Leu-L-(S-benzyl)cysteine-L-Phe-L-Ser-L-Lys-L-Gln-2,4-dinitrophenyl-ethylenediamine + H2O - Homo sapiens o-aminobenzoic acid-L-Lys-L-Leu-L-(S-benzyl)cysteine-L-Phe-L-Ser-L-Lys-L-Gln + 2,4-dinitrophenyl-ethylenediamine - ? 366601 3.4.22.1 o-aminobenzoic acid-L-Lys-L-Leu-L-Arg-L-Phe-L-Ser-L-Lys-L-Gln-2,4-dinitrophenyl-ethylenediamine + H2O - Homo sapiens o-aminobenzoic acid-L-Lys-L-Leu-L-Arg-L-Phe-L-Ser-L-Lys-L-Gln + 2,4-dinitrophenyl-ethylenediamine - ? 366606 3.4.22.1 o-aminobenzoic acid-L-Lys-L-Leu-L-Phe-L-Phe-L-Ser-L-Lys-L-Gln-2,4-dinitrophenyl-ethylenediamine + H2O - Homo sapiens o-aminobenzoic acid-L-Lys-L-Leu-L-Phe-L-Phe-L-Ser-L-Lys-L-Gln + 2,4-dinitrophenyl-ethylenediamine - ? 366605 3.4.22.1 o-aminobenzoyl-L-Phe-L-Arg-L-Lys-epsilon-N-2,4-dinitrophenylamide + H2O - Homo sapiens ? - ? 366590 3.4.22.1 ovalbumin + H2O - Angiostrongylus cantonensis ? - ? 37064 3.4.22.1 Phe-Lys-4-aminobenzyloxycarbonyl-doxorubicin prodrug-albumin + H2O albumin-binding prodrug of doxorubicin, which incorporates a maleimide moiety and a 4-aminobenzyloxycarbonyl spacer coupled to the dipeptide Phe-Lys, is cleaved by cathepsin B and in tumor homogenates of MDA-MB 435 tumor cells from in vitro culture transplanted subcutaneously into the left flank region of mice, overview Homo sapiens ? - ? 402836 3.4.22.1 Phe-Lys-4-aminobenzyloxycarbonyl-doxorubicin prodrug-albumin + H2O albumin-binding prodrug of doxorubicin, which incorporates a maleimide moiety and a 4-aminobenzyloxycarbonyl spacer coupled to the dipeptide Phe-Lys, is cleaved by cathepsin B Homo sapiens ? - ? 402836 3.4.22.1 phenylacetyl-L-Arg-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens phenylacetyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 366704 3.4.22.1 phenylacetyl-L-Phe-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens phenylacetyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 366708 3.4.22.1 pro-rhoptry protein 2 + H2O - Toxoplasma gondii rhoptry protein 2 + propeptide - ? 366591 3.4.22.1 Protamine + H2O - Artemia sp. Hydrolyzed protamine - ? 15478 3.4.22.1 protein rGSII-D88N + H2O - Callosobruchus maculatus ? - ? 406510 3.4.22.1 protein rGSII-D88N + H2O degradation Callosobruchus maculatus ? - ? 406510 3.4.22.1 Proteins + H2O - Mus musculus ? - ? 369561 3.4.22.1 Proteins + H2O - Homo sapiens ? - ? 369561 3.4.22.1 Proteins + H2O - Rattus norvegicus ? - ? 369561 3.4.22.1 Proteins + H2O - Bos taurus ? - ? 369561 3.4.22.1 Proteins + H2O - Oryctolagus cuniculus ? - ? 369561 3.4.22.1 Proteins + H2O - Tetrahymena pyriformis ? - ? 369561 3.4.22.1 Proteins + H2O - Hemicentrotus pulcherrimus ? - ? 369561 3.4.22.1 Proteins + H2O - Capra hircus ? - ? 369561 3.4.22.1 Proteins + H2O - Schistosoma mansoni ? - ? 369561 3.4.22.1 Proteins + H2O - Cyprinus carpio ? - ? 369561 3.4.22.1 Proteins + H2O - Artemia sp. ? - ? 369561 3.4.22.1 Proteins + H2O - Dorytheuthis bleekeri ? - ? 369561 3.4.22.1 Proteins + H2O - Bubalus bubalis ? - ? 369561 3.4.22.1 Proteins + H2O - Heliocidaris crassispina ? - ? 369561 3.4.22.1 Proteins + H2O - Fasciola hepatica ? - ? 369561 3.4.22.1 Proteins + H2O - Scomber australasicus ? - ? 369561 3.4.22.1 Proteins + H2O - Ancylostoma caninum ? - ? 369561 3.4.22.1 Proteins + H2O degradation of tissue proteins within lysosomes Sus scrofa ? - ? 369561 3.4.22.1 Proteins + H2O turnover of intracellular proteins Gallus gallus ? - ? 369561 3.4.22.1 Proteins + H2O turnover of intracellular proteins Rattus norvegicus ? - ? 369561 3.4.22.1 Proteins + H2O turnover of intracellular proteins Sus scrofa ? - ? 369561 3.4.22.1 Proteins + H2O turnover of intracellular proteins Bos taurus ? - ? 369561 3.4.22.1 Proteins + H2O turnover of intracellular proteins Capra hircus ? - ? 369561 3.4.22.1 Proteins + H2O turnover of intracellular proteins Bubalus bubalis ? - ? 369561 3.4.22.1 Proteins + H2O muscle proteins Macaca fuscata ? - ? 369561 3.4.22.1 Proteins + H2O plays a role in cancer invasion and metastasis Homo sapiens ? - ? 369561 3.4.22.1 Proteins + H2O enzyme activation and conversion of precursor proteins to their active form Capra hircus ? - ? 369561 3.4.22.1 proteoglycans + H2O - Homo sapiens hydrolyzed proteoglycans - ? 36985 3.4.22.1 serum albumin + H2O - Schistosoma mansoni partially degraded serum albumin - ? 36983 3.4.22.1 sheep immunoglobulin heavy chain + H2O - Fasciola hepatica hydrolyzed sheep immunoglobulin heavy chain - ? 36978 3.4.22.1 sphingosine kinase-1 + H2O cleaves at histidine 122 and arginine 199 Homo sapiens ? - ? 393532 3.4.22.1 Suc-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O 6% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Trachurus japonicus Suc-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 412904 3.4.22.1 TNF-alpha + H2O - Mus musculus ? - ? 398430 3.4.22.1 TNF-alpha + H2O - Homo sapiens ? - ? 398430 3.4.22.1 TNF-alpha + H2O cathepsin B is required for optimal posttranslational processing of TNF-alpha Homo sapiens ? - ? 398430 3.4.22.1 TNF-alpha + H2O cathepsin B is required for optimal posttranslational processing of TNF-alpha in response to the bacterial cell wall component lipopolysacchrides Mus musculus ? - ? 398430 3.4.22.1 TNF-alpha + H2O - Mus musculus C57BL/6 ? - ? 398430 3.4.22.1 TNF-alpha + H2O cathepsin B is required for optimal posttranslational processing of TNF-alpha in response to the bacterial cell wall component lipopolysacchrides Mus musculus C57BL/6 ? - ? 398430 3.4.22.1 Tos-Gly-Pro-Arg-4-methyl-7-amidocoumarin + H2O - Homo sapiens Tos-Gly-Pro-Arg + 4-methyl-7-aminocoumarin - ? 412985 3.4.22.1 Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O - Fasciola hepatica Tos-Gly-Pro-Arg + 7-amino-4-methylcoumarin - ? 412986 3.4.22.1 trypsinogen + H2O - Bos taurus trypsin + peptide - ? 36967 3.4.22.1 trypsinogen + H2O 1 mM DTT, 1 mM EDTA, pH 4.1, room temperature Homo sapiens trypsin + ? - ? 74459 3.4.22.1 trypsinogen activation peptide + H2O 1 mM DTT, 1 mM EDTA, pH 4.1, room temperature Homo sapiens ? - ? 385857 3.4.22.1 Type I collagen + H2O - Fasciola gigantica ? - ? 16730 3.4.22.1 yolk protein + H2O - Fundulus heteroclitus ? - ? 393866 3.4.22.1 Z-Arg-Arg-7-amido-4-methylcoumarin + H2O - Fundulus heteroclitus Z-Arg-Arg + 7-amino-4-methylcoumarin - ? 385950 3.4.22.1 Z-Arg-Arg-7-amido-4-methylcoumarin + H2O 1 mM DTT, 2 mM EDTA, pH 6.8, 37°C, activity varied from 0.013 to 3.4 U/mg protein in normal tissue and from 0.007 to 20.0 U/mg protein in cancer tissue Homo sapiens Z-Arg-Arg + 7-amino-4-methylcoumarin - ? 385950 3.4.22.1 Z-Arg-Arg-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature Trichobilharzia regenti Z-Arg-Arg + 7-amino-4-methylcoumarin - ? 385950 3.4.22.1 Z-Arg-Arg-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, least preferred substrate Trichobilharzia regenti Z-Arg-Arg + 7-amino-4-methylcoumarin - ? 385950 3.4.22.1 Z-Arg-Arg-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, negligible hydrolysis Trichobilharzia regenti Z-Arg-Arg + 7-amino-4-methylcoumarin - ? 385950 3.4.22.1 Z-Arg-Arg-7-amido-4-methylcoumarin + H2O 20 mM DTT, pH 5.5 Rattus norvegicus Z-Arg-Arg + 7-amino-4-methylcoumarin - ? 385950 3.4.22.1 Z-Arg-Arg-7-amido-4-methylcoumarin + H2O 37°C, pH 6.0, 8 mM DTT, 352 mM KH2PO4, 48 mM Na2HPO4, 4 mM disodium EDTA Rattus norvegicus Z-Arg-Arg + 7-amino-4-methylcoumarin - ? 385950 3.4.22.1 Z-Arg-Arg-7-amido-4-methylcoumarin + H2O pH 5, 1 mM EDTA, 4 mM DTT Mus musculus Z-Arg-Arg + 7-amino-4-methylcoumarin - ? 385950 3.4.22.1 Z-Arg-Arg-cresyl violet + H2O - Homo sapiens Z-Arg-Arg + cresyl violet - ? 385951 3.4.22.1 Z-Arg-Arg-p-nitroanilide + H2O 1 microM, pH 6.0, 10 nanoM cathepsin B Homo sapiens ? - ? 406909 3.4.22.1 Z-Arg-Leu-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature Trichobilharzia regenti Z-Arg-Leu + 7-amino-4-methylcoumarin - ? 385952 3.4.22.1 Z-Arg-Leu-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, less than 25% hydrolysis compared to Z-Arg-Val-7-amido-4-methylcoumarin Trichobilharzia regenti Z-Arg-Leu + 7-amino-4-methylcoumarin - ? 385952 3.4.22.1 Z-Arg-Phe-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature Trichobilharzia regenti Z-Arg-Phe + 7-amino-4-methylcoumarin - ? 385953 3.4.22.1 Z-Arg-Phe-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, less than 25% hydrolysis compared to Z-Arg-Val-7-amido-4-methylcoumarin Trichobilharzia regenti Z-Arg-Phe + 7-amino-4-methylcoumarin - ? 385953 3.4.22.1 Z-Arg-Val-7-amido-4-methylcoumarin + H2O 2 mM DTT, pH 3.0-8.0, 0.3 M NaCl, room temperature, preferred substrate Trichobilharzia regenti Z-Arg-Val + 7-amino-4-methylcoumarin - ? 385955 3.4.22.1 Z-arginyl-arginyl-7-amido-4-methylcoumarin + H2O 37°C, pH 5.5, 5 mM DTT Uronema marinum Z-Arg-Arg + 7-amino-4-methylcoumarin - ? 385956 3.4.22.1 Z-L-Arg-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens Z-L-Arg-L-Arg + 7-amino-4-methylcoumarin - ? 366705 3.4.22.1 Z-L-Phe-L-Arg 7-amido-4-methylcoumarin + H2O - Homo sapiens Z-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 434477 3.4.22.1 Z-L-Phe-L-Arg 7-amido-4-methylcoumarin + H2O - Larimichthys crocea Z-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 434477 3.4.22.1 Z-L-Phe-L-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens Z-L-Phe-L-Arg + 7-amino-4-methylcoumarin - ? 366709 3.4.22.1 Z-Phe-Arg-4-methylcoumarin-7-amide + H2O - Bos taurus Z-Phe-Arg + 7-amino-4-methylcoumarin - ? 385968 3.4.22.1 Z-Phe-Arg-4-methylcoumarin-7-amide + H2O - Homo sapiens Z-Phe-Arg + 7-amino-4-methylcoumarin - ? 385968