3.4.22.10 90% renaturation provided the denatured zymogen is solubilized with urea or guanidinium chloride and given time to refold 3.4.22.10 refolding of denatured recombinant mature wild-type enzyme from inclusion bodies in 100 mM Na-acetate, pH 4.5, 8 M urea, 10 mM DTT, and 280 mM NaCl, by rapid dilution 1:20 into phosphate buffered saline, pH 7.4, with 10 mM DTT