3.1.1.61 - 3.1.1.61 recombinant enzyme 3.1.1.61 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 3.1.1.61 strain ST89pGK2, plasmid bearing strain overproducing esterase and transferase