3.4.22.B50 additional information - additional information Michaelis-Menten steady-state enzyme-kinetics of wild-type and mutant enzymes, overview 731069 3.4.22.B50 0.0186 - Dabcyl-FRLKGGAPIKGV-Edans wild-type, pH 6.5, 30°C 752517 3.4.22.B50 0.021 - Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 5.25 667619 3.4.22.B50 0.0321 - Dabcyl-FRLKGGAPIKGV-Edans mutant T39W, pH 6.5, 30°C 752517 3.4.22.B50 0.0396 - Dabcyl-FKKKGGGDVKE-Edans mutant T39W, pH 6.5, 30°C 752517 3.4.22.B50 0.0616 - Dabcyl-FKKKGGGDVKE-Edans wild-type, pH 6.5, 30°C 752517 3.4.22.B50 0.064 - Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 6.05 667619 3.4.22.B50 0.0654 - Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 6.82 667619 3.4.22.B50 0.068 - Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 9.2 667619 3.4.22.B50 0.0704 - Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 9.8 667619 3.4.22.B50 0.0714 - Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 8.65 667619 3.4.22.B50 0.072 - Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 8.01 667619 3.4.22.B50 0.0772 - Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 7.42 667619 3.4.22.B50 0.09687 - N-benzoyl-Phe-Val-Arg-4-nitroanilide mutant enzyme S32T/A67Y, in 50 mM Na-acetate buffer pH 5.0, at 60°C 732725 3.4.22.B50 0.1273 - N-benzoyl-Phe-Val-Arg-4-nitroanilide wild type enzyme, in 50 mM Na-acetate buffer pH 5.0, at 60°C 732725 3.4.22.B50 0.1499 - N-benzoyl-Phe-Val-Arg-4-nitroanilide mutant enzyme S32T, in 50 mM Na-acetate buffer pH 5.0, at 60°C 732725 3.4.22.B50 0.1769 - N-benzoyl-Phe-Val-Arg-4-nitroanilide mutant enzyme A67Y, in 50 mM Na-acetate buffer pH 5.0, at 60°C 732725 3.4.22.B50 0.185 - FRLKGGAPIKGV - 667619 3.4.22.B50 0.67 - Z-LRGG-7-amido-4-methylcoumarin in 20 mM Tris-HCl, pH 7.5, 0.1 mg/ml BSA, 150 mM NaCl, 2 mM dithiothreitol, at 25°C 732186 3.4.22.B50 0.73 - ubiquitin-7-amido-4-trifluoromethylcoumarin recombinant wild-type enzyme, pH 7.4, 30°C 731069 3.4.22.B50 1.01 - ubiquitin-7-amido-4-trifluoromethylcoumarin recombinant mutant E168R, pH 7.4, 30°C 731069 3.4.22.B50 1.1 - ubiquitin-7-amido-4-trifluoromethylcoumarin recombinant mutant E168D, pH 7.4, 30°C 731069 3.4.22.B50 1.2 - ubiquitin-7-amido-4-trifluoromethylcoumarin recombinant mutant L163Q, pH 7.4, 30°C 731069 3.4.22.B50 1.26 - ubiquitin-7-amido-4-trifluoromethylcoumarin recombinant mutant E168A, pH 7.4, 30°C 731069 3.4.22.B50 1.35 - ubiquitin-7-amido-4-trifluoromethylcoumarin recombinant mutant Y265F, pH 7.4, 30°C 731069 3.4.22.B50 2.48 - ubiquitin-7-amido-4-trifluoromethylcoumarin recombinant mutant Y265A, pH 7.4, 30°C 731069 3.4.22.B50 3.23 - Z-KKAG-7-amido-4-methylcoumarin in 20 mM Tris-HCl, pH 7.5, 0.1 mg/ml BSA, 150 mM NaCl, 2 mM dithiothreitol, at 25°C 732186 3.4.22.B50 3.33 - ubiquitin-7-amido-4-trifluoromethylcoumarin recombinant mutant D165A, pH 7.4, 30°C 731069