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Literature summary extracted from

  • Kim, N.Y.; Lee, Y.J.; Park, J.W.; Kim, S.N.; Kim, E.Y.; Kim, Y.; Kim, O.B.
    An Escherichia coli FdrA variant derived from syntrophic coculture with a methanogen increases succinate production due to changes in allantoin degradation (2021), mSphere, 6, e0065421.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.5 D296Y naturally occurring mutation, the FdrAD296Y variant increases glyoxylate conversion to malate by accelerating oxamate production, which promotes carbon flow through the C4 branch, leading to increased succinate production Escherichia coli
2.1.3.5 additional information wild-type Escherichia coli is adapted to syntrophic growth with Methanobacterium formicicum for glycerol fermentation over 44 weeks leading to rapid evolution through repeated emergence of mutators that introduced a large number of nucleotide variants. Many mutators increase to high frequencies but remain polymorphic throughout the continuous cultivation. The evolved Escherichia coli populations exhibit gains both in fitness and succinate production, but only for growth under glycerol fermentation with Methanobacterium formicicum (laboratory conditions) and not under other growth conditions. Mutant alleles of the 69 single nucleotide polymorphisms (SNPs) are identified in the adapted Escherichia coli populations, analysis of phenotypic changes caused by 84 variants, including 15 nonsense variants, revealing that FdrAD296Y is the most significant variant leading to increased succinate production, overview Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.5 phosphate + N-carbamoyl-2-oxoglycine Escherichia coli
-
 carbamoyl phosphate + oxamate
-
 r
2.1.3.5 phosphate + N-carbamoyl-2-oxoglycine Escherichia coli K12
-
 carbamoyl phosphate + oxamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.5 Escherichia coli Q47208
-
-
2.1.3.5 Escherichia coli K12 Q47208
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.5 phosphate + N-carbamoyl-2-oxoglycine
-
 Escherichia coli carbamoyl phosphate + oxamate
-
 r
2.1.3.5 phosphate + N-carbamoyl-2-oxoglycine
-
 Escherichia coli K12 carbamoyl phosphate + oxamate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.1.3.5 AllF SwissProt Escherichia coli
2.1.3.5 FdrA
-
 Escherichia coli
2.1.3.5 ocarbamoyltransferase subunit AllF SwissProt Escherichia coli

Expression

EC Number Organism Comment Expression
2.1.3.5 Escherichia coli transcription of fdrA is induced under anaerobic allantoin degradation conditions up

General Information

EC Number General Information Comment Organism
2.1.3.5 metabolism the anaerobic allantoin degradation pathway involving the enzyme is linked to succinate production via the glyoxylate pathway during glycerol fermentation. Wild-type Escherichia coli is limited in producing succinate from glycerol as the sole carbon source under anaerobic conditions because it is energetically unfavorable, while Escherichia coli FdrAD296Y mutant increases glyoxylate conversion to malate by accelerating oxamate production, which promotes carbon flow through the C4 branch, leading to increased succinate production Escherichia coli
2.1.3.5 physiological function FdrA plays a crucial role in oxamate production. FdrA catalyzes a global prokaryote orphan reaction for anaerobic allantoin degradation, a model pathway through glyoxylate leading to succinate production under conditions of glycerol fermentation is presented Escherichia coli