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Literature summary extracted from

  • Chang, Z.; Liu, D.; Yang, Z.; Wu, J.; Zhuang, W.; Niu, H.; Ying, H.
    Efficient xylitol production from cornstalk hydrolysate using engineered Escherichia coli whole cells (2018), J. Agric. Food Chem., 66, 13209-13216 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.431 gene xyl1, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with glucose dehydrogenase (GDH) gene of Bacillus cereus strain ATCC 14579 Yamadazyma tenuis
1.1.1.431 gene xyr8, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with glucose dehydrogenase (GDH) gene of Bacillus cereus strain ATCC 14579 Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.431 D-xylose + NADPH + H+ Yamadazyma tenuis
-
xylitol + NADP+
-
r
1.1.1.431 D-xylose + NADPH + H+ Candida tropicalis
-
xylitol + NADP+
-
r
1.1.1.431 D-xylose + NADPH + H+ Yamadazyma tenuis CBS 4435
-
xylitol + NADP+
-
r
1.1.1.431 D-xylose + NADPH + H+ Candida tropicalis IFO 0618
-
xylitol + NADP+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.431 Candida tropicalis P87039
-
-
1.1.1.431 Candida tropicalis IFO 0618 P87039
-
-
1.1.1.431 Yamadazyma tenuis O74237
-
-
1.1.1.431 Yamadazyma tenuis CBS 4435 O74237
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.431 D-xylose + NADPH + H+
-
Yamadazyma tenuis xylitol + NADP+
-
r
1.1.1.431 D-xylose + NADPH + H+
-
Candida tropicalis xylitol + NADP+
-
r
1.1.1.431 D-xylose + NADPH + H+
-
Yamadazyma tenuis CBS 4435 xylitol + NADP+
-
r
1.1.1.431 D-xylose + NADPH + H+
-
Candida tropicalis IFO 0618 xylitol + NADP+
-
r
1.1.1.431 additional information analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose Yamadazyma tenuis ?
-
-
1.1.1.431 additional information analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose Candida tropicalis ?
-
-
1.1.1.431 additional information analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose Yamadazyma tenuis CBS 4435 ?
-
-
1.1.1.431 additional information analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose Candida tropicalis IFO 0618 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.431 NAD(P)H-dependent D-xylose reductase UniProt Yamadazyma tenuis
1.1.1.431 NADPH-dependent D-xylose reductase II,III UniProt Candida tropicalis
1.1.1.431 Texr
-
Yamadazyma tenuis
1.1.1.431 TrxR
-
Candida tropicalis
1.1.1.431 XYL1
-
Yamadazyma tenuis
1.1.1.431 xyr8
-
Candida tropicalis
1.1.1.431 XyrB
-
Candida tropicalis

Temperature Optimum [┬░C]

EC Number Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
1.1.1.431 25
-
assay at Yamadazyma tenuis
1.1.1.431 25
-
assay at Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.431 7
-
assay at Yamadazyma tenuis
1.1.1.431 7
-
assay at Candida tropicalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.431 NADP+
-
Yamadazyma tenuis
1.1.1.431 NADP+
-
Candida tropicalis
1.1.1.431 NADPH
-
Yamadazyma tenuis
1.1.1.431 NADPH
-
Candida tropicalis

General Information

EC Number General Information Comment Organism
1.1.1.431 physiological function xylitol production through whole-cell catalysis from pure xylose and glucose, and xylitol production from cornstalk hydrolysate Yamadazyma tenuis
1.1.1.431 physiological function xylitol production through whole-cell catalysis from pure xylose and glucose, and xylitol production from cornstalk hydrolysate, method optimization, overview Candida tropicalis