Literature summary extracted from

Chu, H.F.; Chen, C.C.; Moses, D.C.; Chen, Y.H.; Lin, C.H.; Tsai, Y.C.; Chou, C.Y.
Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine (2018), Antiviral Res., 158, 199-205 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.22.B50	expression in Escherichia coli	porcine epidemic diarrhea virus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.4.22.B50	modeling of structure	porcine epidemic diarrhea virus

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.4.22.B50	T39W	mutation enhances hydrolysis of the SARS-CoV-derived peptidyl substrate Dabcyl-FRLKGGAPIKGV-Edans	porcine epidemic diarrhea virus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.22.B50	6-Mercaptopurine	-	porcine epidemic diarrhea virus
3.4.22.B50	6-thioguanine	noncompetitive	porcine epidemic diarrhea virus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.4.22.B50	0.0186	-	Dabcyl-FRLKGGAPIKGV-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus
3.4.22.B50	0.0321	-	Dabcyl-FRLKGGAPIKGV-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus
3.4.22.B50	0.0396	-	Dabcyl-FKKKGGGDVKE-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus
3.4.22.B50	0.0616	-	Dabcyl-FKKKGGGDVKE-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.22.B50	25000	-	gel filtration	porcine epidemic diarrhea virus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.B50	porcine epidemic diarrhea virus	P0C6Y4	polyprotein	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.22.B50	Dabcyl-FKKKGGGDVKE-Edans + H2O	-	porcine epidemic diarrhea virus	?	-	?
3.4.22.B50	Dabcyl-FRLKGGAPIKGV-Edans + H2O	SARS-CoV-derived substrate	porcine epidemic diarrhea virus	?	-	?
3.4.22.B50	ubiquitin-7-amido-4-trifluoro-methyl-coumarin + H2O	-	porcine epidemic diarrhea virus	ubiquitin + 7-amino-4-trifluoro-methyl-coumarin	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.4.22.B50	monomer	1 * 25800, calculated from sequence	porcine epidemic diarrhea virus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.4.22.B50	0.0011	-	Dabcyl-FRLKGGAPIKGV-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus
3.4.22.B50	0.0041	-	Dabcyl-FKKKGGGDVKE-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus
3.4.22.B50	0.005	-	Dabcyl-FKKKGGGDVKE-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus
3.4.22.B50	0.0175	-	Dabcyl-FRLKGGAPIKGV-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.4.22.B50	0.0211	-	6-thioguanine	pH 6.5, 30°C	porcine epidemic diarrhea virus

IC50 Value

EC Number	IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
3.4.22.B50	0.0137	-	pH 6.5, 30°C	porcine epidemic diarrhea virus	6-thioguanine
3.4.22.B50	0.0581	-	pH 6.5, 30°C	porcine epidemic diarrhea virus	6-Mercaptopurine

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3.4.22.B50	0.055	-	Dabcyl-FRLKGGAPIKGV-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus
3.4.22.B50	0.058	-	Dabcyl-FRLKGGAPIKGV-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus
3.4.22.B50	0.082	-	Dabcyl-FKKKGGGDVKE-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus
3.4.22.B50	0.103	-	Dabcyl-FKKKGGGDVKE-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus

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Release 2023.1 (January 2023)