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Literature summary extracted from

  • Chou, Y.W.; Cheng, S.C.; Lai, H.Y.; Chou, C.Y.
    Differential domain structure stability of the severe acute respiratory syndrome coronavirus papain-like protease (2012), Arch. Biochem. Biophys., 520, 74-80.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.2 expressed in Escherichia coli Alphacoronavirus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.2 Urea the reversible unfolding/refolding process of the PLpro in the presence of urea is investigated. The zinc-binding domain of the enzyme starts to unfold at urea concentration below 0.35 M and reaches a first plateau at 1-3.5 M. At this stage, the palm and thumb core domains remain well folded but totally inactive Alphacoronavirus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 0.73
-
 ubiquitin-7-amido-4-trifluoromethylcoumarin pH not specified in the publication, 30°C, PLpro(1541-1858, DNA fragment) Alphacoronavirus
3.4.22.2 1.84
-
 ubiquitin-7-amido-4-trifluoromethylcoumarin pH not specified in the publication, 30°C, PLpro(1600-1858, DNA fragment) Alphacoronavirus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Alphacoronavirus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 ubiquitin-7-amido-4-trifluoromethylcoumarin + H2O
-
 Alphacoronavirus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.2 papain-like protease
-
 Alphacoronavirus
3.4.22.2 PLpro
-
 Alphacoronavirus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.2 30
-
 assay at Alphacoronavirus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.2 0.79
-
 ubiquitin-7-amido-4-trifluoromethylcoumarin pH not specified in the publication, 30°C, PLpro(1541-1858, DNA fragment) Alphacoronavirus
3.4.22.2 0.96
-
 ubiquitin-7-amido-4-trifluoromethylcoumarin pH not specified in the publication, 30°C, PLpro(1600-1858, DNA fragment) Alphacoronavirus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.2 0.52
-
 ubiquitin-7-amido-4-trifluoromethylcoumarin pH not specified in the publication, 30°C, PLpro(1600-1858, DNA fragment) Alphacoronavirus
3.4.22.2 1.08
-
 ubiquitin-7-amido-4-trifluoromethylcoumarin pH not specified in the publication, 30°C, PLpro(1541-1858, DNA fragment) Alphacoronavirus