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Literature summary extracted from

  • Tan, T.; Spadiut, O.; Wongnate, T.; Sucharitakul, J.; Krondorfer, I.; Sygmund, C.; Haltrich, D.; Chaiyen, P.; Peterbauer, C.; Divne, C.
    The 1.6 A crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate specificity and reveals a flavin intermediate (2013), PLoS ONE, 8, e53567.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.29 expressed in Pichia pastoris Macropsalliota meleagris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.99.29 microseeding, using 50 mM KH2PO4, 5% (v/v) 2-methyl-2,4-pentanediol, and 20% (w/v) polyethylene glycol 8000 Macropsalliota meleagris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.29 a pyranoside + acceptor Macropsalliota meleagris
-
 a 3-dehydropyranoside + reduced acceptor 3-dehydropyranoside or 3,4-didehydropyranoside ?
1.1.99.29 D-glucose + acceptor Macropsalliota meleagris the enzyme oxidizes D-glucose at both the C2 and C3 position but the C2 position is the primary site of attack 2-dehydro-D-glucose + reduced acceptor
-
 ?
1.1.99.29 additional information Macropsalliota meleagris O2 is a poor electron acceptor ?
-
 ?
1.1.99.29 pyranose + acceptor Macropsalliota meleagris
-
 2-dehydropyranose + reduced acceptor 2-dehydropyranose or 1-dehydropyranose or 3-dehydropyranose or 2,3-didehydropyranose ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.29 Macropsalliota meleagris Q3L245
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.29 hydrophobic interaction chromatography and anion exchange chromatography Macropsalliota meleagris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.29 3-fluoro-3-deoxy-D-glucose + acceptor
-
 Macropsalliota meleagris ? + reduced acceptor
-
 ?
1.1.99.29 a pyranoside + acceptor
-
 Macropsalliota meleagris a 3-dehydropyranoside + reduced acceptor 3-dehydropyranoside or 3,4-didehydropyranoside ?
1.1.99.29 D-glucose + acceptor the enzyme oxidizes D-glucose at both the C2 and C3 position but the C2 position is the primary site of attack Macropsalliota meleagris 2-dehydro-D-glucose + reduced acceptor
-
 ?
1.1.99.29 additional information O2 is a poor electron acceptor Macropsalliota meleagris ?
-
 ?
1.1.99.29 pyranose + acceptor
-
 Macropsalliota meleagris 2-dehydropyranose + reduced acceptor 2-dehydropyranose or 1-dehydropyranose or 3-dehydropyranose or 2,3-didehydropyranose ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.29 PDH
-
 Macropsalliota meleagris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.29 FAD
-
 Macropsalliota meleagris