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Literature summary extracted from

  • Brown, D.M.; Upcroft, J.A.; Upcroft, P.
    A H2O-producing NADH oxidase from the protozoan parasite Giardia duodenalis (1996), Eur. J. Biochem., 241, 155-161.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.3.2 CuSO4 0.2 mM, 97% inhibition Giardia duodenalis
1.6.3.2 H2O2 0.5 mM, 50% inhibition Giardia duodenalis
1.6.3.2 HgCl2 0.5 mM, 80% inhibition Giardia duodenalis
1.6.3.2 additional information no inhibition by EDTA Giardia duodenalis
1.6.3.2 p-chloromercuribenzoate 0.05 mM, complete inhibition Giardia duodenalis
1.6.3.2 Quinacrine 1 mM, 73% inhibition Giardia duodenalis
1.6.3.2 Quinine 0.2 mM, 50% inhibition Giardia duodenalis
1.6.3.2 ZnSO4 0.1 mM, 98% inhibition Giardia duodenalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.3.2 0.0042
-
 NADH pH 7.8, 22°C Giardia duodenalis
1.6.3.2 0.016
-
 NADPH pH 7.8, 22°C Giardia duodenalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.3.2 additional information activity is not enhanced by the addition of various heavy metals which suggests that the enzyme does not contain any metal ions as co-factors Giardia duodenalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.3.2 45116
-
 1 * 45116, calculated from sequence Giardia duodenalis
1.6.3.2 46000
-
 1 * 46000, SDS-PAGE Giardia duodenalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.3.2 2 NADPH + 2 H+ + O2 Giardia duodenalis the enzyme may be involved in maintenance of an optimum intracellular redox ratio 2 NADP+ + 2 H2O
-
 ?
1.6.3.2 2 NADPH + 2 H+ + O2 Giardia duodenalis BRIS/83/HEPU/106 the enzyme may be involved in maintenance of an optimum intracellular redox ratio 2 NADP+ + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.2 Giardia duodenalis Q7M4I9
-
-
1.6.3.2 Giardia duodenalis BRIS/83/HEPU/106 Q7M4I9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.3.2
-
 Giardia duodenalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.3.2 trophozoite
-
 Giardia duodenalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.2 2 NADH + H+ + O2 NADPH is more efficient as electron donor compared to NADH Giardia duodenalis NAD+ + 2 H2O
-
 ?
1.6.3.2 2 NADH + H+ + O2 NADPH is more efficient as electron donor compared to NADH Giardia duodenalis BRIS/83/HEPU/106 NAD+ + 2 H2O
-
 ?
1.6.3.2 2 NADPH + 2 H+ + O2 the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia duodenalis 2 NADP+ + 2 H2O
-
 ?
1.6.3.2 2 NADPH + 2 H+ + O2 O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically Giardia duodenalis 2 NADP+ + 2 H2O
-
 ?
1.6.3.2 2 NADPH + 2 H+ + O2 the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia duodenalis BRIS/83/HEPU/106 2 NADP+ + 2 H2O
-
 ?
1.6.3.2 2 NADPH + 2 H+ + O2 O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically Giardia duodenalis BRIS/83/HEPU/106 2 NADP+ + 2 H2O
-
 ?
1.6.3.2 additional information cytochrome c is not reduced under any assay conditions used Giardia duodenalis ?
-
 ?
1.6.3.2 additional information cytochrome c is not reduced under any assay conditions used Giardia duodenalis BRIS/83/HEPU/106 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.3.2 monomer 1 * 46000, SDS-PAGE Giardia duodenalis
1.6.3.2 monomer 1 * 45116, calculated from sequence Giardia duodenalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.3.2 7.8
-
-
 Giardia duodenalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.3.2 7 9 active over the range pH 7-9 Giardia duodenalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.3.2 FAD FAD is noncoveltly bound, 1 mol of FAD per mol of polypeptide Giardia duodenalis
1.6.3.2 NADH NADPH is more efficient as electron donor compared to NADH Giardia duodenalis
1.6.3.2 NADPH NADPH is more efficient as electron donor compared to NADH Giardia duodenalis

General Information

EC Number General Information Comment Organism
1.6.3.2 physiological function the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia duodenalis