Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Petschacher, B.; Nidetzky, B.
    Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae (2008), Microb. Cell Fact., 7, 9.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 overexpression of the enzyme in Saccharomyces cerevisiae strain CEN.PK 113-7D under control of the constitutive TDH3 promoter, co-expression with xylose reductase from Candida tenuis Candida sp. HA 167
1.1.1.430 overexpression of wild-type enzyme and mutant enzyme, with modified cofactor specificity, in Saccharomyces cerevisiae strain CEN.PK 113-7D under control of the constitutive TDH3 promoter, coexpression with xylitol dehydrogenase from Galactocandida mastotermitis, The strain harboring the xylose reductase double mutant shows 42% enhanced ethanol yield compared to the reference strain harboring wild-type xylose reductase during anaerobic bioreactor conversions of xylose. Likewise, the yields of xylitol and glycerol are decreased by 52% and 57% respectively in the xylose reductase mutant strain Yamadazyma tenuis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.430 K274R/N276D structure-guided site-directed mutagenesis, change of the coenzyme preference of the xyluose reductase about 170fold from NADPH in the wild-type to NADH, which, in spite of the structural modifications introduced, has retained the original catalytic efficiency for reduction of xylose by NADH Yamadazyma tenuis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 xylitol + NAD+ Candida sp. HA 167
-
 D-xylulose + NADH + H+
-
 ?
1.1.1.430 D-xylose + NAD(P)H + H+ Yamadazyma tenuis
-
 xylitol + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Candida sp. HA 167
-
-
-
1.1.1.430 Yamadazyma tenuis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 xylitol + NAD+
-
 Candida sp. HA 167 D-xylulose + NADH + H+
-
 ?
1.1.1.430 D-xylose + NAD(P)H + H+
-
 Yamadazyma tenuis xylitol + NAD(P)+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 XDH
-
 Candida sp. HA 167
1.1.1.9 xylitol dehydrogenase
-
 Candida sp. HA 167
1.1.1.430 xylose reductase
-
 Yamadazyma tenuis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+
-
 Candida sp. HA 167
1.1.1.430 NAD(P)H
-
 Yamadazyma tenuis