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Literature summary extracted from

  • Hashizume, T.; Xu, Y.; Mohutsky, M.A.; Alberts, J.; Hadden, C.; Kalhorn, T.F.; Isoherranen, N.; Shuhart, M.C.; Thummel, K.E.
    Identification of human UDP-glucuronosyltransferases catalyzing hepatic 1alpha,25-dihydroxyvitamin D3 conjugation (2008), Biochem. Pharmacol., 75, 1240-1250.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.17 hecogenin a selective UGT1A4 inhibitor, complete inhibition of UGT1A4 at 0.05 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 0.0073
-
 1alpha,25-dihydroxyvitamin D3 25-O-glucuronidation of 1alpha,25-dihydroxyvitamin D3 by UGT1A4 supersomes Homo sapiens
2.4.1.17 0.0112
-
 1alpha,25-dihydroxyvitamin D3 25-O-glucuronidation of 1alpha,25-dihydroxyvitamin D3 by liver microsomes Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 microsome
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.17 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.17 UDP-D-glucuronate + acceptor Homo sapiens
-
 UDP + acceptor beta-D-glucuronoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Homo sapiens P22310 isozyme UGT1A4
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.17 0.0000329
-
 25-O-glucuronidation of 1alpha,25-dihydroxyvitamin D3 by liver microsomes Homo sapiens
2.4.1.17 0.0000337
-
 25-O-glucuronidation of 1alpha,25-dihydroxyvitamin D3 by UGT1A4 supersomes Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 additional information substrate concentration-rate profiles of 25-O-glucuronide formation by UGT1A4, UGT2B4, and UGT2B7, overview Homo sapiens ?
-
 ?
2.4.1.17 UDP-D-glucuronate + 1alpha,25-dihydroxyvitamin D3 among 12 different UGT isozymes tested, only UGT1A4, and also UGT2B4 and UGT2B7 support the reaction, the main product by UGT1A4 supersomes and human liver microsomes is the 25-O-glucuronide, product identification and determination by mass spectral and NMR analyses, overview Homo sapiens UDP + 1alpha,25-dihydroxyvitamin D3 25-O-beta-D-glucuronoside
-
 ?
2.4.1.17 UDP-D-glucuronate + acceptor
-
 Homo sapiens UDP + acceptor beta-D-glucuronoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.17 UDP-glucuronosyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.17 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.17 7.4
-
 assay at Homo sapiens