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Literature summary extracted from

  • Lenz, O.; Zebger, I.; Hamann, J.; Hildebrandt, P.; Friedrich, B.
    Carbamoylphosphate serves as the source of CN(-), but not of the intrinsic CO in the active site of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha (2007), FEBS Lett., 581, 3322-3326.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.99.6 expressed in Escherichia coli JM109 cells Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 cytoplasm
-
 Cupriavidus necator 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Iron within the catalytic centre one carbonyl and two cyanide ligands are covalently attached to the iron Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Cupriavidus necator
-
-
-
1.12.99.6 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 Strep-Tactin Superflow column chromatography Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + acceptor
-
 Cupriavidus necator H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + acceptor
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 H+ + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 regulatory hydrogenase
-
 Cupriavidus necator
1.12.99.6 [NiFe]-hydrogenase
-
 Cupriavidus necator