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Literature summary extracted from

  • Lindner, H.A.; Fotouhi-Ardakani, N.; Lytvyn, V.; Lachance, P.; Sulea, T.; Menard, R.
    The papain-like protease from the severe acute respiratory syndrome coronavirus is a deubiquitinating enzyme (2005), J. Virol., 79, 15199-15208.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.22.B50 medicine viral strategy to modulate the host cell ubiquitination machinery to its advantage Alphacoronavirus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B50 expression in Escherichia coli BL21 (DE3) and HeLa cells Alphacoronavirus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.B50 C1651A Plpro active-site mutant Alphacoronavirus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B50 Ubiquitin aldehyde
-
 Alphacoronavirus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B50 38000
-
 SDS-PAGE and Western blot detection with His6-specific antibodies, P-Plpro(C1)-His6 fragment Alphacoronavirus
3.4.22.B50 41000
-
 SDS-PAGE and Western blot detection with His6-specific antibodies, P-Plpro(C2)-His6 fragment Alphacoronavirus
3.4.22.B50 60000
-
 SDS-PAGE and Western blot detection with His6-specific antibodies, T7-ISG15P-Plpro(C2)-His6 fragment Alphacoronavirus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B50 Alphacoronavirus
-
 SARS-CoV
-
3.4.22.B50 Alphacoronavirus SARS-CoV
-
 SARS-CoV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B50 by His-Bind Purification Kit Alphacoronavirus

Storage Stability

EC Number Storage Stability Organism
3.4.22.B50 -80°C, Tris-HCl buffer, pH 8.0, 10% glycerol, 1 month Alphacoronavirus
3.4.22.B50 4°C, Tris-HCl buffer, pH 8.0, 10% glycerol, 24 h Alphacoronavirus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B50 branched polyubiquitin chains + H2O
-
 Alphacoronavirus ?
-
 ?
3.4.22.B50 branched polyubiquitin chains + H2O
-
 Alphacoronavirus SARS-CoV ?
-
 ?
3.4.22.B50 ISG15-nsp2-fusion protein + H2O
-
 Alphacoronavirus ?
-
 ?
3.4.22.B50 ISG15-nsp2-fusion protein + H2O
-
 Alphacoronavirus SARS-CoV ?
-
 ?
3.4.22.B50 ubiquitin-7-amido-4-methylcoumarin + H2O
-
 Alphacoronavirus ?
-
 ?
3.4.22.B50 ubiquitin-7-amido-4-methylcoumarin + H2O
-
 Alphacoronavirus SARS-CoV ?
-
 ?
3.4.22.B50 Z-LRGG-7-amido-4-methylcoumarin + H2O
-
 Alphacoronavirus ?
-
 ?
3.4.22.B50 Z-LRGG-7-amido-4-methylcoumarin + H2O
-
 Alphacoronavirus SARS-CoV ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B50 papain-like proteinase
-
 Alphacoronavirus
3.4.22.B50 PLpro
-
 Alphacoronavirus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.B50 0.00021
-
 Ubiquitin aldehyde
-
 Alphacoronavirus