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Literature summary extracted from

  • Barretto, N.; Jukneliene, D.; Ratia, K.; Chen, Z.; Mesecar, A.D.; Baker, S.C.
    The papain-like protease of severe acute respiratory syndrome coronavirus has deubiquitinating activity (2005), J. Virol., 79, 15189-15198.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B50 expression in Escherichia coli XL-1 Blue and HeLa-MHV receptor cells Alphacoronavirus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.B50 C1651A purified SARS-CoV PLpro protein containing an alanine substitution at putative catalytic residues Alphacoronavirus
3.4.22.B50 C1732A encodes a substitution of a cysteine residue, predicted to be critical for zinc binding Alphacoronavirus
3.4.22.B50 D1826A purified SARS-CoV PLpro protein containing an alanine substitution at putative catalytic residues Alphacoronavirus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B50 35000
-
-
 Alphacoronavirus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B50 Alphacoronavirus
-
 SARS-CoV
-
3.4.22.B50 Alphacoronavirus SARS-CoV
-
 SARS-CoV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B50 purified to homogeneity by hydrophobic interaction and anion exchange chromatography Alphacoronavirus

Storage Stability

EC Number Storage Stability Organism
3.4.22.B50 -80°C, Tris buffer, pH 7.5, 20% glycerol Alphacoronavirus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B50 (E-EDANS)RELNGGAPI(K-DABCYL)S + H2O
-
 Alphacoronavirus ?
-
 ?
3.4.22.B50 (E-EDANS)RELNGGAPI(K-DABCYL)S + H2O
-
 Alphacoronavirus SARS-CoV ?
-
 ?
3.4.22.B50 diubiquitin + H2O
-
 Alphacoronavirus ?
-
 ?
3.4.22.B50 diubiquitin + H2O
-
 Alphacoronavirus SARS-CoV ?
-
 ?
3.4.22.B50 ubiquitin-7-amido-4-methylcoumarin + H2O
-
 Alphacoronavirus ?
-
 ?
3.4.22.B50 ubiquitin-7-amido-4-methylcoumarin + H2O
-
 Alphacoronavirus SARS-CoV ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B50 papain-like proteinase
-
 Alphacoronavirus
3.4.22.B50 PLpro
-
 Alphacoronavirus