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Literature summary extracted from
- Coronavirus papain-like endopeptidases (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 1265-1269.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.22.B14 | - |
Murine hepatitis virus |
| 3.4.22.B50 | vaccinia-T1-mediated expression of a cDNA clone encoding the MHV PLP2 domain | Alphacoronavirus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.B14 | E-64d | membrane-permeable do the irreversible cysteine proteinase inhibitor E-64 blocks cleavage of p65 in vivo | Murine hepatitis virus |  |
| 3.4.22.B14 | leupeptin | blocks the ability of PLP1 to cleave p28 in vitro but not in vivo due to permeability and toxicity issues | Murine hepatitis virus | |
| 3.4.22.B14 | ZnCl2 | blocks the ability of PLP1 to cleave p28 in vitro but not in vivo due to permeability and toxicity issues | Murine hepatitis virus | |
| 3.4.22.B50 | additional information | not sensitive to cysteine protease inhibitor E-64d | Alphacoronavirus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.B14 | 28000 | - |
- |
Murine hepatitis virus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.B14 | p28 | Murine hepatitis virus | - |
? | - |
? | |
| 3.4.22.B14 | p65 | Murine hepatitis virus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.B14 | Murine hepatitis virus | - |
- |
- |
| 3.4.22.B50 | Alphacoronavirus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.B14 | - |
Murine hepatitis virus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.B14 | p28 | - |
Murine hepatitis virus | ? | - |
? | |
| 3.4.22.B14 | p65 | - |
Murine hepatitis virus | ? | - |
? | |
| 3.4.22.B50 | PLP2-MP1 precursor polyprotein + H2O | - |
Alphacoronavirus | MP1 + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.22.B14 | first papain-like cysteine proteinase | - |
Murine hepatitis virus |
| 3.4.22.B14 | PLP1 | - |
Murine hepatitis virus |
| 3.4.22.B50 | papain-like cysteine proteinase | - |
Alphacoronavirus |
| 3.4.22.B50 | PLP2 | - |
Alphacoronavirus |
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