Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Han, Y.S.; Chang, G.G.; Juo, C.G.; Lee, H.J.; Yeh, S.H.; Hsu, J.T.; Chen, X.
    Papain-like protease 2 (PLP2) from severe acute respiratory syndrome coronavirus (SARS-CoV): expression, purification, characterization, and inhibition (2005), Biochemistry, 44, 10349-10359.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.B50 medicine antiviral therapy Alphacoronavirus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B50 cloned into modified version of the pBacPAK8/His2 vector and expressed in insect cells Sf9 Alphacoronavirus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B50 Cu2+ weakly inhibited by 70% at 0.01 mM Alphacoronavirus
3.4.22.B50 hydroxypridine-2-thione Zn
-
 Alphacoronavirus
3.4.22.B50 additional information not inhibited by Mg2+, Mn2+, Ca2+, Ni2+ and Co2+ at 0.01 mM, E64, leupeptin and antipain at 0.1 mM, N-ethylmaleimide and chymostatin at 1 mM and cystatin at 0.01 mg/mL respectively Alphacoronavirus
3.4.22.B50 N-ethyl-N-phenyldithiocarbaic acid Zn
-
 Alphacoronavirus
3.4.22.B50 Zn2+
-
 Alphacoronavirus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.B50 0.021
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 5.25 Alphacoronavirus
3.4.22.B50 0.064
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 6.05 Alphacoronavirus
3.4.22.B50 0.0654
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 6.82 Alphacoronavirus
3.4.22.B50 0.068
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 9.2 Alphacoronavirus
3.4.22.B50 0.0704
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 9.8 Alphacoronavirus
3.4.22.B50 0.0714
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 8.65 Alphacoronavirus
3.4.22.B50 0.072
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 8.01 Alphacoronavirus
3.4.22.B50 0.0772
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 7.42 Alphacoronavirus
3.4.22.B50 0.185
-
 FRLKGGAPIKGV
-
 Alphacoronavirus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B50 51000
-
 SDS-PAGE Alphacoronavirus
3.4.22.B50 56700
-
 gel filtration Alphacoronavirus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B50 Alphacoronavirus
-
 SARS-CoV
-
3.4.22.B50 Alphacoronavirus SARS-CoV
-
 SARS-CoV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B50 gel filtration Alphacoronavirus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B50 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O fluorogenic substrate Alphacoronavirus ?
-
 ?
3.4.22.B50 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O fluorogenic substrate Alphacoronavirus SARS-CoV ?
-
 ?
3.4.22.B50 BCoV substrate
-
 Alphacoronavirus ?
-
 ?
3.4.22.B50 BCoV substrate
-
 Alphacoronavirus SARS-CoV ?
-
 ?
3.4.22.B50 FRLKGGAPIKGV 12 mer oligopeptide containing Gly818-Ala819, 100% cleavage Alphacoronavirus ?
-
 ?
3.4.22.B50 FRLKGGAPIKGV 12 mer oligopeptide containing Gly818-Ala819, 100% cleavage Alphacoronavirus SARS-CoV ?
-
 ?
3.4.22.B50 ISLKGGKIVSTC 12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage Alphacoronavirus ?
-
 ?
3.4.22.B50 ISLKGGKIVSTC 12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage Alphacoronavirus SARS-CoV ?
-
 ?
3.4.22.B50 MHV substrate
-
 Alphacoronavirus ?
-
 ?
3.4.22.B50 additional information SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates Alphacoronavirus ?
-
 ?
3.4.22.B50 additional information SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates Alphacoronavirus SARS-CoV ?
-
 ?
3.4.22.B50 RELNGGAVTRYV + H2O 12 mer oligopeptide containing Gly180-Ala181, 5% cleavage Alphacoronavirus AVTYRV + RELNGG
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.B50 monomer
-
 Alphacoronavirus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B50 papain-like protease
-
 Alphacoronavirus
3.4.22.B50 PLP2
-
 Alphacoronavirus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.B50 37
-
 thermolabil, significantly reduced activity after incubation at temperatures higher than 37°C for 30 min Alphacoronavirus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.B50 0.023
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 5.25 Alphacoronavirus
3.4.22.B50 0.025
-
 FRLKGGAPIKGV
-
 Alphacoronavirus
3.4.22.B50 0.1
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 9.8 Alphacoronavirus
3.4.22.B50 0.198
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 6.05 Alphacoronavirus
3.4.22.B50 0.233
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 9.2 Alphacoronavirus
3.4.22.B50 0.33
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 7.42 Alphacoronavirus
3.4.22.B50 0.343
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 6.82 Alphacoronavirus
3.4.22.B50 0.363
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 8.01 Alphacoronavirus
3.4.22.B50 0.366
-
 Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 8.65 Alphacoronavirus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.B50 6.8 8.6
-
 Alphacoronavirus