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Literature summary extracted from

  • Snijder, E.J.; Wassenaar, A.L.; van Dinten, L.C.; Spaan, W.J.; Gorbalenya, A.E.
    The arterivirus nsp4 protease is the prototype of a novel group of chymotrypsin-like enzymes, the 3C-like serine proteases (1996), J. Biol. Chem., 271, 4864-4871.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.114
-
 equine arteritis virus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.114 D1129E mutant is able to cleave the Nsp4/5 site with wild-type efficiently but does not process the Nsp3/4 and Nsp5/6 junctions equine arteritis virus
3.4.21.114 D1129K no Nsp3456 cleavage equine arteritis virus
3.4.21.114 D1129V no Nsp3456 cleavage equine arteritis virus
3.4.21.114 H1103G complete inhibition of Nsp3456 processing equine arteritis virus
3.4.21.114 H1103R complete inhibition of Nsp3456 processing equine arteritis virus
3.4.21.114 H1198L completely abolished processing of Nsp4/5 and Nsp5/6 equine arteritis virus
3.4.21.114 H1198R completely abolished processing of Nsp4/5 and Nsp5/6 equine arteritis virus
3.4.21.114 H1198Y completely abolished processing of Nsp4/5 and Nsp5/6 equine arteritis virus
3.4.21.114 S1184C mutation has no effect on Nsp3456 processing equine arteritis virus
3.4.21.114 S1184F mutation has no effect on Nsp3456 processing equine arteritis virus
3.4.21.114 S1184I mutation has no effect on Nsp3456 processing equine arteritis virus
3.4.21.114 S1184Y mutation has no effect on Nsp3456 processing equine arteritis virus
3.4.21.114 T1179D completely abolished processing of Nsp4/5 and Nsp5/6 equine arteritis virus
3.4.21.114 T1179G reduced cleavage of Nsp4/5, no cleavage of Nsp5/6 site equine arteritis virus
3.4.21.114 T1179N retains wild-type activity towards Nsp4/5, cleaves Nsp5/6 with increased efficiency equine arteritis virus
3.4.21.114 T1179S reduced cleavage of Nsp4/5, no cleavage of Nsp5/6 site equine arteritis virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.114 21000
-
 calculated from amino acid sequence equine arteritis virus
3.4.21.114 31000
-
 SDS-PAGE equine arteritis virus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.114 equine arteritis virus P19811 Bucyrus strain
-
3.4.21.114 equine arteritis virus Bucyrus P19811 Bucyrus strain
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.114 proteolytic modification replicase of EAV processed by at least 3 viral proteases, resulting in the generation of non-structural proteins Nsp1-6 equine arteritis virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.114 arterivirus replicase ORF1a protein + H2O
-
 equine arteritis virus ?
-
 ?
3.4.21.114 arterivirus replicase ORF1a protein + H2O
-
 equine arteritis virus Bucyrus ?
-
 ?
3.4.21.114 additional information nsp4 cleaves multiple GluX(Gly/Ser/Ala) sites, X: cleavage site equine arteritis virus ?
-
 ?
3.4.21.114 additional information nsp4 cleaves multiple GluX(Gly/Ser/Ala) sites, X: cleavage site equine arteritis virus Bucyrus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.114 nsp4
-
 equine arteritis virus