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Literature summary extracted from

  • Cameron, M.D.; Aust, S.D.
    Kinetics and reactivity of the flavin and heme cofactors of cellobiose dehydrogenase from Phanerochaete chrysosporium (2000), Biochemistry, 39, 13595-13601.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.18 extracellular
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 Phanerodontia chrysosporium
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-

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Phanerodontia chrysosporium Q01738
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18
-
 Phanerodontia chrysosporium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.18 culture medium
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 Phanerodontia chrysosporium
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + acceptor Cytochrome c, 2,6-dichlorophenolindophenol, ferricyanide, 3,5-di-tert-butyl-1,2-benzoquinone, Mn3+-malonate can serve as electron acceptors. O2 is a poor acceptor in the absence of artificial acceptors and is reduced to H2O2. Molecular oxygen acts as two-electron acceptor, superoxide is produced through an iron mediated pathway Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced acceptor
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 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 16
-
 cytochrome c
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 Phanerodontia chrysosporium
1.1.99.18 74
-
 FAD reduction rate increases with increasing concententrations of the electron acceptors methyl-1,4-benzoquinone or Fe3+ Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 flavin flavin cofactor within cellobiose dehydrogenase is able to reduce all electron acceptors tested. The addition of electron acceptors increases the rate of flavin reduction and the electron transfer rate between the flavin and heme. The addition of ferric iron eliminates the flavin radical present in reduced cellobiose dehydrogenase, while it increases the flavin radical ESR signal in the independent flavin domain. No radical is detected with either cellobiose dehydrogenase or the flavin domain upon the addition of methyl-1,4-benzoquinone. Oxygen behaves like a two-electron acceptor. Superoxide production is found only upon the inclusion of iron Phanerodontia chrysosporium