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Literature summary extracted from

  • Castillo-Villanueva, A.; Mendez, S.T.; Torres-Arroyo, A.; Reyes-Vivas, H.; Oria-Hernandez, J.
    Cloning, expression and characterization of recombinant, NADH oxidase from Giardia lamblia (2016), Protein J., 35, 24-33.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.3.2 additional information not activated by dithiothreitol Giardia duodenalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.3.2 expressed in Escherichia coli BL21(DE3)pLysS cells Giardia duodenalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.3.2 Cu2+ 92% inhibition at 0.1 mg/ml Giardia duodenalis
1.6.3.2 Hg2+ complete inhibition at 0.1 mg/ml Giardia duodenalis
1.6.3.2 p-mercuribenzoate 99% inhibition at 0.1 mg/ml Giardia duodenalis
1.6.3.2 Quinacrine 51% inhibition at 0.1 mg/ml Giardia duodenalis
1.6.3.2 Zn2+ 89% inhibition at 0.1 mg/ml Giardia duodenalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.3.2 0.017
-
 NADH at pH 7.8 and 25°C Giardia duodenalis
1.6.3.2 0.0874
-
 NADPH at pH 7.8 and 25°C Giardia duodenalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.3.2 214000
-
 gel filtration Giardia duodenalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.3.2 NADH + H+ + O2 Giardia duodenalis
-
 NAD+ + H2O
-
 ?
1.6.3.2 NADPH + H+ + O2 Giardia duodenalis
-
 NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.2 Giardia duodenalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.3.2 Ni-Sepharose column chromatography Giardia duodenalis

Storage Stability

EC Number Storage Stability Organism
1.6.3.2 -70°C, 50% (v/v) glycerol, at least 2 months, no loss of activity Giardia duodenalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.2 NADH + H+ + O2
-
 Giardia duodenalis NAD+ + H2O
-
 ?
1.6.3.2 NADPH + H+ + O2
-
 Giardia duodenalis NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.3.2 homotetramer 4 * 50300, calculated from amino acid sequence Giardia duodenalis
1.6.3.2 homotetramer 4 * 50000, SDS-PAGE Giardia duodenalis

Synonyms

EC Number Synonyms Comment Organism
1.6.3.2 NOX
-
 Giardia duodenalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.3.2 7.8
-
-
 Giardia duodenalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.3.2 FAD the enzyme contains 0.91 mol FAD per mol of monomer Giardia duodenalis