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Literature summary for 7.2.2.19 extracted from

  • Swarts, H.G.; Koenderink, J.B.; Willems, P.H.; De Pont, J.J.
    The human non-gastric H,K-ATPase has a different cation specificity than the rat enzyme (2007), Biochim. Biophys. Acta, 1768, 580-589.
    View publication on PubMed
Search for more literature for 7.2.2.19

Cloned(Commentary)

Cloned (Comment) Organism
expression of non-gastric enzyme in Spodoptera frugiperda Sf9 cells via baculovirus transfection, co-expression with the Rattus norvegicus beta1 subunit of Na,K-ATPase Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
oligomycin increases the phosphorylation of the enzyme and the sensitivity of the phosphorylated intermediate to AD inhibiting the conversion of E1P to E2P Homo sapiens
oubain
-
 Homo sapiens
SCH 28080 inhibits the NH4+-stimulated activity Homo sapiens
vanadate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 ATP pH 7.0, 37°C, recombinant enzyme, in presence of 100 mM Na+ Homo sapiens
0.4
-
 ATP pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+ Homo sapiens
1
-
 K+ pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM K+ Homo sapiens
1.2
-
 ATP pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM K+ Homo sapiens
2
-
 K+ pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+ Homo sapiens
20
-
 K+ pH 7.0, 37°C, recombinant enzyme, in presence of 100 mM Na+ Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ low activation by K+, but stimulates enzyme dephosphorylation Homo sapiens
Mg2+ required for ATPase activity Homo sapiens
Na+ activates, stimulates enzyme dephosphorylation Homo sapiens
NH4+ best activating cation, stimulates enzyme dephosphorylation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in + K+/out Homo sapiens
-
 ADP + phosphate + H+/out + K+/in
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the steady-state phopshorylation level of the enzyme decreases with increasing pH, K+ and Na+ concentration, but nearly doubles in presence of oligomycin, Na+, K+, and NH4+ stimulate enzyme dephosphorylation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant non-gastric enzyme partially from Spodoptera frugiperda Sf9 cells by membrane preparation Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
stomach
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.186
-
 partially purified recombinant enzyme, in presence of 10 mM K+ Homo sapiens
0.611
-
 partially purified recombinant enzyme, in presence of 100 mM Na+ Homo sapiens
0.685
-
 partially purified recombinant enzyme, in presence of 10 mM NH4+ Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in + K+/out
-
 Homo sapiens ADP + phosphate + H+/out + K+/in
-
 ?

Synonyms

Synonyms Comment Organism
additional information the enzyme belongs to the P-type ATPases Homo sapiens
non-gastric H,K-ATPase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0008
-
 pH 7.0, 37°C, recombinant enzyme, in presence of 100 mM Na+ Homo sapiens vanadate
0.0013
-
 pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+ Homo sapiens vanadate
0.0016
-
 pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM K+ Homo sapiens vanadate
0.003
-
 pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+, or 10 mM K+, or 100 mM Na+ Homo sapiens oligomycin
0.02
-
 pH 7.0, 37°C, recombinant enzyme, in presence of 100 mM Na+ Homo sapiens SCH 28080
0.03
-
 pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+ Homo sapiens SCH 28080
0.06
-
 pH 7.0, 37°C, recombinant enzyme, in presence of 100 mM Na+ Homo sapiens oubain
0.4
-
 pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+ Homo sapiens oubain
4
-
 above, pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM K+ Homo sapiens SCH 28080
8
-
 above, pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM K+ Homo sapiens oubain