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Literature summary for 4.1.1.4 extracted from

  • Yang, H.; Huang, B.; Lai, N.; Gu, Y.; Li, Z.; Ye, Q.; Wu, H.
    Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate (2019), Microb. Cell Fact., 18, 0000.
    View publication on PubMed
Search for more literature for 4.1.1.4

Application

Application Comment Organism
synthesis the engineered Escherichia coli strain with optimized hybrid acetone biosynthetic pathway can utilize acetate gas substrate efficiently to synthesize acetone without other non-gas byproducts providing a potential method for industrial biomanufacturing of acetone by engineered Escherichia coli strains from non-food based substrate Clostridium acetobutylicum

Cloned(Commentary)

Cloned (Comment) Organism
gene adc, recombinant expression in Escherichia coli, coexpression with gene thlA encoding for thiolase, gene ctfAB encoding for coenzyme A transferase, and Escherichia coli MG1655 genes atoB, encoding acetyl-CoA acetyltransferase, and atoDA, encoding for acetyl-CoA:acetoacetyl-CoA transferase subunit alpha and beta Clostridium acetobutylicum

Protein Variants

Protein Variants Comment Organism
additional information metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate, simplified metabolic pathways of acetone biosynthesis by engineered Escherichia coli strain using acetate as carbon source under aerobic condition, overview. In the optimized hybrid acetone biosynthesis pathway, acetate is first converted into acetyl-CoA through ACS (acetyl-CoA synthetase) pathway or ACK-PTA pathway. Then, 2 mol of acetyl-CoA are condensed by thiolase to generate 1 mol of acetoacetyl-CoA. Acetoacetyl-CoA transferase transfers the CoA moiety from acetoacetyl-CoA to acetate, and forms acetoacetate and acetyl-CoA, which is part of endogenous acetate utilization pathway. Finally, acetoacetate is catalyzed to form acetone and carbon dioxide by acetoacetate decarboxylase. Construction of recombinant Escherichia coli strain MG1655 expressing the hybrid pathway consisting of thlA, atoDA, and adc, that produces high levels of acetone from acetate. Reducing the gluconeogenesis pathway has little effect on acetone production, while blocking the TCA cycle by knocking out the icdA gene enhances the yield of acetone significantly. As a result, acetone concentration increases up to 113.18 mM in 24 h by the resting cell culture coupling with gas-stripping methods. The engineered Escherichia coli strain with optimized hybrid acetone biosynthetic pathway can utilize acetate gas substrate efficiently to synthesize acetone without other non-gas byproducts Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetoacetate + H+ Clostridium acetobutylicum
-
 acetone + CO2
-
 ?
acetoacetate + H+ Clostridium acetobutylicum DSM 792
-
 acetone + CO2
-
 ?
acetoacetate + H+ Clostridium acetobutylicum JCM 1419
-
 acetone + CO2
-
 ?
acetoacetate + H+ Clostridium acetobutylicum LMG 5710
-
 acetone + CO2
-
 ?
acetoacetate + H+ Clostridium acetobutylicum VKM B-1787
-
 acetone + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum P23670
-
-
Clostridium acetobutylicum DSM 792 P23670
-
-
Clostridium acetobutylicum JCM 1419 P23670
-
-
Clostridium acetobutylicum LMG 5710 P23670
-
-
Clostridium acetobutylicum VKM B-1787 P23670
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetate + H+
-
 Clostridium acetobutylicum acetone + CO2
-
 ?
acetoacetate + H+
-
 Clostridium acetobutylicum DSM 792 acetone + CO2
-
 ?
acetoacetate + H+
-
 Clostridium acetobutylicum JCM 1419 acetone + CO2
-
 ?
acetoacetate + H+
-
 Clostridium acetobutylicum LMG 5710 acetone + CO2
-
 ?
acetoacetate + H+
-
 Clostridium acetobutylicum VKM B-1787 acetone + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ADC
-
 Clostridium acetobutylicum

General Information

General Information Comment Organism
metabolism the enzyme is involved in acetone biosynthesis Clostridium acetobutylicum