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Literature summary for 3.6.4.13 extracted from
- The C-terminal region of the RNA helicase CshA is required for the interaction with the degradosome and turnover of bulk RNA in the opportunistic pathogen Staphylococcus aureus (2015), RNA Biol., 12, 658-674 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cshA, recombinant expression of FLAG-Strep-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta (DE3) | Staphylococcus aureus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Staphylococcus aureus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Staphylococcus aureus | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Staphylococcus aureus NCTC 8325 | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | Q2FWH5 | - |
- |
| Staphylococcus aureus NCTC 8325 | Q2FWH5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant FLAG-Strep-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) by two steps of affinity chromatography, recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Staphylococcus aureus | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Staphylococcus aureus NCTC 8325 | ADP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CshA | - |
Staphylococcus aureus |
| DEAD-box RNA helicase | - |
Staphylococcus aureus |
| RNA helicase CshA | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at, ATPase assay | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at, ATPase assay | Staphylococcus aureus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | a selected subset of RNAs is significantly stabilised in absence of the RNA helicase | Staphylococcus aureus |
| additional information | the C-terminal region of the RNA helicase CshA is required for the interaction with the degradosome and turnover of bulk RNA in the opportunistic pathogen Staphylococcus aureus. Half-lives of diverse RNAs in the wild-type and mutant CshA strains, correlation of half life and steady-state levels, detailed overview | Staphylococcus aureus |
| physiological function | CshA is required for efficient turnover of the bulk of mRNAs. For efficient degradation, the RNA helicase interacts through its C-terminal extension with the degradosome components. The molecular function of the RNA helicase might be the destabilisation of secondary structures or the removal of hindering RNA binding proteins | Staphylococcus aureus |
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