Cloned (Comment) | Organism |
---|---|
phylogenetic analysis | Arabidopsis thaliana |
phylogenetic analysis | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of double knockdown mutant rh3-4/clpr2-1, chloroplast rps12-int1 splicing defects in mutant rh3-4 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
chloroplast | stroma as well as thylakoid membranes | Zea mays | 9507 | - |
chloroplast stroma | - |
Zea mays | 9570 | - |
additional information | enzyme RH3 accumulates in stroma and nucleoids of green tissues, with peak accumulation during chloroplast biogenesis | Arabidopsis thaliana | - |
- |
additional information | ZmRH3 associates with 50S preribosome particles as well as nucleoids. Enzyme RH3 accumulates in stroma and nucleoids of green tissues, with peak accumulation during chloroplast biogenesis | Zea mays | - |
- |
thylakoid membrane | - |
Zea mays | 42651 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8L7S8 | - |
- |
Zea mays | A0A1D6GDY8 | ZmRH3A | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | AtRH3 is predominantly expressed in young leaves, peaking in 2-week-old seedlings and decreasing to very low levels in older leaf tissue | Arabidopsis thaliana | - |
leaf | the enzyme abundance is high in the sink-source transition zone of developing maize leaves, coincident with the plastid biogenesis machinery | Zea mays | - |
additional information | enzyme RH3 accumulates in stroma and nucleoids of green tissues, with peak accumulation during chloroplast biogenesis | Zea mays | - |
additional information | enzyme RH3 accumulates in stroma and nucleoids of green tissues, with peak accumulation during chloroplast biogenesis. No enzyme activity in stems or roots of seedlings | Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
Subunits | Comment | Organism |
---|---|---|
More | domain organization of RH3 protein | Arabidopsis thaliana |
More | domain organization of RH3 protein | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
AtRH3 | - |
Arabidopsis thaliana |
DEAD box RNA helicase | - |
Arabidopsis thaliana |
DEAD box RNA helicase | - |
Zea mays |
ZmRH3 | - |
Zea mays |
ZmRH3A | - |
Zea mays |
ZmRH3B | - |
Zea mays |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | 5 to 10fold upregulation of enzyme AtRH3 in plastid caseinolytic protease mutants. AtRH3 up-regulation is not a direct consequence of reduced proteolysis but constitutes a compensatory response at both RH3 transcript and protein levels to impaired chloroplast biogenesis. RH3 up-regulation is nt specific for ClpPR core protease mutants but is a general result of defects in chloroplast biogenesis | up |
General Information | Comment | Organism |
---|---|---|
malfunction | AtRH3 null mutants are embryo lethal, whereas a weak allele results in pale-green seedlings with defects in splicing of several group II introns and rRNA maturation as well as reduced levels of assembled ribosomes,phenotype overview | Arabidopsis thaliana |
additional information | domain organization of RH3 protein | Zea mays |
additional information | domain organization of RH3 protein. RH3 and ClpR2 interact genetically, but RH3 is unlikely to be a substrate for the Clp protease | Arabidopsis thaliana |
physiological function | the enzyme is under strong developmental control, and its abundance sharply peaks in the sink-source transition zone of developing maize leaves, coincident with the plastid biogenesis machinery | Zea mays |
physiological function | the enzyme is under strong developmental control. Cross talk between the chloroplast and the nucleus is used to regulate RH3 levels. Enzyme RH3 functions in the splicing of group II introns and possibly also contributes to the assembly of the 50S ribosomal particle | Arabidopsis thaliana |