Cloned (Comment) | Organism |
---|---|
overexpression of the DEADbox mitif II mutant enzyme in N2a cells leading to reduced Let-7a activity | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | trageting of the enzyme DDX6 with shRNA in N2a cells. Construction of a mutant DDX6 that contains a point mutation within the DEAD box motif II of DDX6 and thus lacks helicase activity, but is still able to coimmunoprecipitate TRIM32 | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | primarily | Mus musculus | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | helicase DDX6 colocalizes with TRIM32 in neural stem cells and neurons and increases the activity of Let-7a. The activation of Let-7a depends on the enzyme's helicase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P54823 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
neural stem cell | TRIM32, DDX6 and Ago2 colocalize | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | helicase DDX6 colocalizes with TRIM32 in neural stem cells and neurons and increases the activity of Let-7a. The activation of Let-7a depends on the enzyme's helicase activity | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA helicase DDX6 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
physiological function | the balance between stem cell maintenance and neuronal differentiation depends on cell-fate determinants such as TRIM32. TRIM32 associates with the RNA-induced silencing complex and increases the activity of microRNAs such as Let-7a. The RNA helicase DDX6 is involved in microRNA regulation. Helicase DDX6 colocalizes with TRIM32 in neural stem cells and neurons and increases the activity of Let-7a. Enzyme DDX6 is necessary and sufficient for neuronal differentiation and it functions in cooperation with TRIM32 | Mus musculus |