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Literature summary for 3.6.4.13 extracted from

  • Nicklas, S.; Okawa, S.; Hillje, A.L.; Gonzalez-Cano, L.; Del Sol, A.; Schwamborn, J.C.
    The RNA helicase DDX6 regulates cell-fate specification in neural stem cells via miRNAs (2015), Nucleic Acids Res., 43, 2638-2654.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the DEADbox mitif II mutant enzyme in N2a cells leading to reduced Let-7a activity Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information trageting of the enzyme DDX6 with shRNA in N2a cells. Construction of a mutant DDX6 that contains a point mutation within the DEAD box motif II of DDX6 and thus lacks helicase activity, but is still able to coimmunoprecipitate TRIM32 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm primarily Mus musculus 5737
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus helicase DDX6 colocalizes with TRIM32 in neural stem cells and neurons and increases the activity of Let-7a. The activation of Let-7a depends on the enzyme's helicase activity ?
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?

Organism

Organism UniProt Comment Textmining
Mus musculus P54823
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Source Tissue

Source Tissue Comment Organism Textmining
brain
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Mus musculus
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neural stem cell TRIM32, DDX6 and Ago2 colocalize Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information helicase DDX6 colocalizes with TRIM32 in neural stem cells and neurons and increases the activity of Let-7a. The activation of Let-7a depends on the enzyme's helicase activity Mus musculus ?
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?

Synonyms

Synonyms Comment Organism
RNA helicase DDX6
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Mus musculus

General Information

General Information Comment Organism
physiological function the balance between stem cell maintenance and neuronal differentiation depends on cell-fate determinants such as TRIM32. TRIM32 associates with the RNA-induced silencing complex and increases the activity of microRNAs such as Let-7a. The RNA helicase DDX6 is involved in microRNA regulation. Helicase DDX6 colocalizes with TRIM32 in neural stem cells and neurons and increases the activity of Let-7a. Enzyme DDX6 is necessary and sufficient for neuronal differentiation and it functions in cooperation with TRIM32 Mus musculus