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Literature summary for 3.6.4.13 extracted from

  • Fiorini, F.; Bagchi, D.; Le Hir, H.; Croquette, V.
    Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities (2015), Nat. Commun., 6, 7581.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.13

Activating Compound

Activating Compound Comment Organism Structure
Upf2 interaction analysis. The two proteins Upf1 and Upf2 form a complex while unwinding RNA. The binding of Upf2 to CH domain activates Upf1-CH-HD unwinding and translocation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His-tagged and N-terminally calmodulin-binding-protein-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Rosetta Homo sapiens

Protein Variants

Protein Variants Comment Organism
F192E site-directed mutagenesis, the mutant shows slightly reduced activity compared with the wild-type enzyme. Upf1F192E definitely prefers to unwind a dsDNA than to translocate it, strand switching Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Homo sapiens
-
 ADP + phosphate
-
 ?
additional information Homo sapiens human Upf1 is able to translocate slowly over long single-stranded nucleic acids with a high processivity. Upf1 efficiently translocates through double-stranded structures and protein-bound sequences. The helicase domain of Upf1 is capable of both unwinding double-stranded nucleic acids and translocation on single-stranded nucleic acids over long distances. Upf1 remodels nucleoprotein complexes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q92900
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged and N-terminally calmodulin-binding-protein-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Rosetta by nickel and calmodulin affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Homo sapiens ADP + phosphate
-
 ?
additional information human Upf1 is able to translocate slowly over long single-stranded nucleic acids with a high processivity. Upf1 efficiently translocates through double-stranded structures and protein-bound sequences. The helicase domain of Upf1 is capable of both unwinding double-stranded nucleic acids and translocation on single-stranded nucleic acids over long distances. Upf1 remodels nucleoprotein complexes Homo sapiens ?
-
 ?
additional information Upf1-HD is a slow translocase compared with other monomeric helicases like UvrD and NS3, but is able to translocate slowly over long single-stranded nucleic acids with a high processivity which exceeds the size of the tested hairpins, overview. Enzyme Upf1 is able to both unwind a long dsRNA of 156 bp and translocate onto ssRNA. Active translocating enzyme Upf1 disrupts protein-nucleic acid interactions. Upf1 remodels nucleoprotein complexes. Substrates are RNA–DNA hybrids, ssDNA and ssRNA Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Upf1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
 RNA assay at Homo sapiens
37
-
 DNA assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information modeling of the late phases of nonsense-mediated mRNA decay Homo sapiens
physiological function Upf1 is a RNA helicase essential for nonsense-mediated mRNA decay, Upf1 is an efficient ribonucleoprotein complex remodeler. Upf1, once recruited onto nonsense-mediated mRNA decay targets, can scan the entire transcript to irreversibly remodel the mRNP, facilitating its degradation by the nonsense-mediated mRNA decay machinery. The ATPase activity of Upf1 is required for the correct progression of nonsense-mediated mRNA decay, a quality-control mechanism that eliminates mRNAs containing premature translation termination codons and also regulates the expression of numerous mRNAs carrying nonsense-mediated mRNA decay target features. The enzyme is specifically recruited to specific targets by an intricate stepwise and translation-dependent pathway Homo sapiens