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Literature summary for 3.6.4.13 extracted from

  • Guo, M.; Hundseth, K.; Ding, H.; Vidhyasagar, V.; Inoue, A.; Nguyen, C.H.; Zain, R.; Lee, J.S.; Wu, Y.
    A distinct triplex DNA unwinding activity of ChlR1 helicase (2015), J. Biol. Chem., 290, 5174-5189.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of FLAG3-tagged wild-type and mutant enzymes in HEK293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K50R site-directed mutagenesis, ATPase dead mutant, fails completely to unwind triplex substrates Homo sapiens
K897del site-directed mutagenesis, the mutant fails in unwinding the DNA substrates Homo sapiens
additional information enzyme inhibition by siRNA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
benzoquinoquinoxaline BQQ, inhibits ChlR1 triplex DNA unwinding activity Homo sapiens
additional information enzyme ChlR1 fails to unwind the triplex substrate in the absence of ATP or in the presence of ADP or ATPgammaS Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Homo sapiens
-
ADP + phosphate
-
?
additional information Homo sapiens ChlR1 robustly unwinds DNA triplex substrates in an ATP-dependent manner requiring an 5'-ssDNA tail, ChlR1 can unwind an intramolecular triplex structure ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96FC9 gene ChlR1 or DDX11
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Homo sapiens ADP + phosphate
-
?
ATP + H2O enzyme ChlR1 fails to unwind the triplex substrate in the absence of ATP or in the presence of ADP or ATPgammaS Homo sapiens ADP + phosphate
-
?
additional information ChlR1 robustly unwinds DNA triplex substrates in an ATP-dependent manner requiring an 5'-ssDNA tail, ChlR1 can unwind an intramolecular triplex structure Homo sapiens ?
-
?
additional information the purified recombinant ChlR1 protein is a DNA-dependent ATPase and unwinds partial duplex DNA substrates with a preferred 5' to 3' directionality, triplex DNA is the preferred DNA substrate for ChlR1, analysis of diverse DNA substrates, overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
ChlR1 helicase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
helicase assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
helicase assay at Homo sapiens

General Information

General Information Comment Organism
evolution ChlR1 is a superfamily 2 DNA helicase that contains a conserved iron-sulfur domain. In humans exist four iron-sulfur DNA helicases, XPD, FANCJ, RTEL1, and ChlR1, that are implicated in autosomal recessive genetic diseases Homo sapiens