Cloned (Comment) | Organism |
---|---|
recombinant expression of FLAG3-tagged wild-type and mutant enzymes in HEK293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K50R | site-directed mutagenesis, ATPase dead mutant, fails completely to unwind triplex substrates | Homo sapiens |
K897del | site-directed mutagenesis, the mutant fails in unwinding the DNA substrates | Homo sapiens |
additional information | enzyme inhibition by siRNA | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoquinoquinoxaline | BQQ, inhibits ChlR1 triplex DNA unwinding activity | Homo sapiens | |
additional information | enzyme ChlR1 fails to unwind the triplex substrate in the absence of ATP or in the presence of ADP or ATPgammaS | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
additional information | Homo sapiens | ChlR1 robustly unwinds DNA triplex substrates in an ATP-dependent manner requiring an 5'-ssDNA tail, ChlR1 can unwind an intramolecular triplex structure | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96FC9 | gene ChlR1 or DDX11 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
ATP + H2O | enzyme ChlR1 fails to unwind the triplex substrate in the absence of ATP or in the presence of ADP or ATPgammaS | Homo sapiens | ADP + phosphate | - |
? | |
additional information | ChlR1 robustly unwinds DNA triplex substrates in an ATP-dependent manner requiring an 5'-ssDNA tail, ChlR1 can unwind an intramolecular triplex structure | Homo sapiens | ? | - |
? | |
additional information | the purified recombinant ChlR1 protein is a DNA-dependent ATPase and unwinds partial duplex DNA substrates with a preferred 5' to 3' directionality, triplex DNA is the preferred DNA substrate for ChlR1, analysis of diverse DNA substrates, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChlR1 helicase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
helicase assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
helicase assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | ChlR1 is a superfamily 2 DNA helicase that contains a conserved iron-sulfur domain. In humans exist four iron-sulfur DNA helicases, XPD, FANCJ, RTEL1, and ChlR1, that are implicated in autosomal recessive genetic diseases | Homo sapiens |