Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | unwinding activity specific for single-strand paired RNA, does not unwind dsRNAs | Thermococcus kodakarensis | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tk-DeaD | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
maximal ATPase activity and unwinding activity specific for single-strand paired RNA | Thermococcus kodakarensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | 40°C: about 40% of maximal activity, 50°C: optimum, 60°C: less than 10% of maximal activity | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | the enzyme starts to unfold at 20°C and fully unfolds at 70°C | Thermococcus kodakarensis |
Organism | Comment | Expression |
---|---|---|
Thermococcus kodakarensis | cold-inducible RNA helicase | up |
Thermococcus kodakarensis | the enzyme is more dominantly transcribed at 60°C than at 85°C and 93°C in both logarithmic and stationary phases. Tk-DeaD is detected only in logarithmic-phase cells cultivated at 60°C but hardly detected at 85°C and 93°C in both phases Tk-DeaD expression is, hence, post-transcriptionally regulated and appears under vigorous growth conditions at 60°C | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme functions under cold stress conditions | Thermococcus kodakarensis |