Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.4.13 extracted from

  • Shimada, Y.; Fukuda, W.; Akada, Y.; Ishida, M.; Nakayama, J.; Imanaka, T.; Fujiwara, S.
    Property of cold inducible DEAD-box RNA helicase in hyperthermophilic archaea (2009), Biochem. Biophys. Res. Commun., 389, 622-627.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O unwinding activity specific for single-strand paired RNA, does not unwind dsRNAs Thermococcus kodakarensis ADP + phosphate
-
?

Synonyms

Synonyms Comment Organism
Tk-DeaD
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
maximal ATPase activity and unwinding activity specific for single-strand paired RNA Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50 40°C: about 40% of maximal activity, 50°C: optimum, 60°C: less than 10% of maximal activity Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 70 the enzyme starts to unfold at 20°C and fully unfolds at 70°C Thermococcus kodakarensis

Expression

Organism Comment Expression
Thermococcus kodakarensis cold-inducible RNA helicase up
Thermococcus kodakarensis the enzyme is more dominantly transcribed at 60°C than at 85°C and 93°C in both logarithmic and stationary phases. Tk-DeaD is detected only in logarithmic-phase cells cultivated at 60°C but hardly detected at 85°C and 93°C in both phases Tk-DeaD expression is, hence, post-transcriptionally regulated and appears under vigorous growth conditions at 60°C up

General Information

General Information Comment Organism
physiological function the enzyme functions under cold stress conditions Thermococcus kodakarensis