Activating Compound | Comment | Organism | Structure |
---|---|---|---|
RNase E | is required for ATPase and RNA unwinding activities of the enzyme, forms a complex with the enzyme, interaction analysis, overview. Avid, enthalpy-favored interaction between the helicase and RNase E 696-762 with an equilibrium binding constant Kaof at least 1 x 108 M-1 determined by isothermal titration calorimetry. Protein-protein and RNA-binding surfaces both communicate allosterically with the ATPase catalytic center | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
construction of a di-cistronic vector that overexpresses a complex comprising RhlB and its recognition site within RNase E, corresponding to residues 696-762, the expression construct is termed pRneRhlBDELTA1-397. Expression of His-tagged wild-type and mutant RhlB and RNaseE in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D310H | site-directed mutagenesis of the V motif, leads to altered enzyme activity, overview | Escherichia coli |
D313H | site-directed mutagenesis of the V motif, leads to altered enzyme activity, overview | Escherichia coli |
H320D | site-directed mutagenesis of the V motif, leads to altered enzyme activity, overview | Escherichia coli |
Y383A | site-directed mutagenesis, the mutation causes the formation of a higher order molecular weight species in binding of RNaseE by RhlB | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
? | |
additional information | Escherichia coli | helicase B, RhlB, is one of the five DEAD box RNA-dependent ATPases in Escherichia coli. ATPases found in Escherichia coli. RhlB requires an interaction with the partner protein RNase E for appreciable ATPase and RNA unwinding activities | ? | - |
? | |
RNA + H2O | Escherichia coli | helicase/unwinding activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant RhlB and RNaseE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000001 | - |
about, wild-type enzyme, RNA helicase activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
additional information | helicase B, RhlB, is one of the five DEAD box RNA-dependent ATPases in Escherichia coli. ATPases found in Escherichia coli. RhlB requires an interaction with the partner protein RNase E for appreciable ATPase and RNA unwinding activities | Escherichia coli | ? | - |
? | |
additional information | RhlB is the only Escherichia coli DEAD box protein that requires a protein partner to stimulate its ATPase activity | Escherichia coli | ? | - |
? | |
RNA + H2O | helicase/unwinding activity | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | location of the nine conserved sequence motifs in the DEAD box helicase RhlB, structure modelling, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
helicase B | - |
Escherichia coli |
RhlB | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |