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Literature summary for 3.6.4.13 extracted from

  • Borowski, P.; Heising, M.V.; Miranda, I.B.; Liao, C.L.; Choe, J.; Baier, A.
    Viral NS3 helicase activity is inhibited by peptides reproducing the Arg-rich conserved motif of the enzyme (motif VI) (2008), Biochem. Pharmacol., 76, 28-38.
    View publication on PubMed

Application

Application Comment Organism
pharmacology peptide inhibitors reproducing the structure of the autoregulatory motif as possibility to develop effective antivirals Hepacivirus C

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Hepacivirus C

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibitory potential of sequences of NTPase/helicase motifs VI derived peptides and their deleted derivatives analyzed, NTP-binding and hydrolyzing site not involved Hepacivirus C

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Hepacivirus C 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Hepacivirus C
-
ADP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, SDS-PAGE Hepacivirus C

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
overview of sequences of NTPase/helicase motifs VI derived peptides and their deleted derivatives, kinetic analyses reveals that binding of the peptides do not interfere with the NTPase activity, peptides do not interact with the ATP binding site Hepacivirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Hepacivirus C ADP + phosphate
-
?
ATP + H2O peptide inhibitors derived from amino acid sequence of motif VI analyzed, binding of the inhibitory peptides does not interfere with the NTPase activity Hepacivirus C ADP + phosphate
-
?

Synonyms

Synonyms Comment Organism
non structural protein 3 ambiguous Hepacivirus C
NTPase/helicase ambiguous Hepacivirus C

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
sequences of NTPase/helicase motifs VI derived peptides and their deleted derivatives analyzed, NTPase activity not affected Hepacivirus C additional information