Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Deoxyribonucleotides | ATPase activity of the bacterially expressed BmL3-helicase is triggered by both the ssRNA and the dsRNA and, to a much lesser extent, by the ssDNA and dsDNA | Brugia malayi | |
Double-stranded DNA | weak stimulation | Brugia malayi | |
Double-stranded RNA | ATPase activity of the recombinant BmL3-helicase is strongly stimulated by dsRNA | Brugia malayi | |
ribonucleotides | ATPase activity of the bacterially expressed BmL3-helicase is triggered by both the ssRNA and the dsRNA and, to a much lesser extent, by the ssDNA and dsDNA, recombinant BmL3-helicase is strongly stimulated by dsRNA | Brugia malayi | |
single-stranded DNA | weak stimulation | Brugia malayi | |
Single-stranded RNA | stimulates | Brugia malayi |
Cloned (Comment) | Organism |
---|---|
expression of the N-terminally His-tagged enzyme as soluble protein in Escherichia coli strain BL21(DE3) | Brugia malayi |
overexpressed as His-tag fusion protein in Escherichia coli | Brugia malayi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Brugia malayi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Brugia malayi | - |
ADP + phosphate | - |
? | |
additional information | Brugia malayi | DEAD box proteins are putative RNA unwinding proteins, BmL3-helicase also is a DEAD box RNA helicase | ? | - |
? | |
RNA + H2O | Brugia malayi | helicase/unwinding activity, either ATP or dATP is required for the unwinding activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brugia malayi | - |
- |
- |
Brugia malayi | EF409381 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Brugia malayi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Brugia malayi | - |
additional information | presence of BmL3-helicase in various life stages of Brugia malayi | Brugia malayi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Brugia malayi | ADP + phosphate | - |
? | |
ATP + H2O | either ATP or dATP is required for the unwinding activity | Brugia malayi | ADP + phosphate | - |
? | |
dATP + H2O | either ATP or dATP is required for the unwinding activity | Brugia malayi | dADP + phosphate | - |
? | |
additional information | DEAD box proteins are putative RNA unwinding proteins, BmL3-helicase also is a DEAD box RNA helicase | Brugia malayi | ? | - |
? | |
RNA + H2O | helicase/unwinding activity, either ATP or dATP is required for the unwinding activity | Brugia malayi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP/dATP-dependent RNA helicase | - |
Brugia malayi |
ATPase/RNA helicase | - |
Brugia malayi |
BmL3-helicase | - |
Brugia malayi |
DEAD box RNA helicase | - |
Brugia malayi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Brugia malayi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Brugia malayi |