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Literature summary for 3.6.4.13 extracted from

  • Yon, C.; Teramoto, T.; Mueller, N.; Phelan, J.; Ganesh, V.K.; Murthy, K.H.; Padmanabhan, R.
    Modulation of the nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase activities of Dengue virus type 2 nonstructural protein 3 (NS3) by interaction with NS5, the RNA-dependent RNA polymerase (2005), J. Biol. Chem., 280, 27412-27419.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information NS3 requires the hydrophilic domain of NS2B for activation Dengue virus type 2
nonstructural protein 5 interaction with NS5, the viral RNA-dependent RNA polymerase, stimulates NS3 NTPAse and RTPase activities as well as thr RNA helicase activity Dengue virus type 2
RNA RNA-stimulated enzyme Dengue virus type 2

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and N-terminally truncated enzyme in Escherichia coli Dengue virus type 2

Protein Variants

Protein Variants Comment Organism
H51A site-directed mutagenesis, the mutant has an inactivated protease domain showing enhanced RNA helicase compared to wild-type full-length enzyme Dengue virus type 2
R184Q/K185N/R186G/K187N construction of the N-terminally truncated mutant NS3DELTA180 containing a mutated RNA substrate biding motif, the mutant shows reduced RTPase activity Dengue virus type 2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study Dengue virus type 2

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Dengue virus type 2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Dengue virus type 2 RNA helicase activity ADP + phosphate
-
?
additional information Dengue virus type 2 nonstructural proteins NS3 and NS5 form complexes in infected mammalian cells ?
-
?

Organism

Organism UniProt Comment Textmining
Dengue virus type 2
-
i.e. DEN2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and N-terminally truncated enzyme from Escherichia coli Dengue virus type 2

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specificities and activities of wild-type and mutant enzymes Dengue virus type 2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O NTPase activity Dengue virus type 2 ADP + phosphate
-
?
ATP + H2O RNA helicase activity Dengue virus type 2 ADP + phosphate
-
?
additional information nonstructural proteins NS3 and NS5 form complexes in infected mammalian cells Dengue virus type 2 ?
-
?
additional information substrate specificity, bifunctional enzyme, NS3 is an RNA-stimulated nucleoside triphosphatase NTPase/RNA helicase and a 5'-RNA triphosphatase RTPase, overview, the full-length NS3 with or without NS2B cofactor domain exhibits a catalytically more efficient RNA helicase activity than the N-terminally-truncated NS3 helicase domain, suggesting that the protease domain enhances RNA helicase activity Dengue virus type 2 ?
-
?

Subunits

Subunits Comment Organism
More DEN2 nonstructural protein 3, NS3, has a serine protease domain and requires the hydrophilic domain of NS2B for activation Dengue virus type 2

Synonyms

Synonyms Comment Organism
nonstructural protein 3
-
Dengue virus type 2
NS3
-
Dengue virus type 2
nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase
-
Dengue virus type 2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Dengue virus type 2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Dengue virus type 2