Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | NS3 requires the hydrophilic domain of NS2B for activation | Dengue virus type 2 | |
nonstructural protein 5 | interaction with NS5, the viral RNA-dependent RNA polymerase, stimulates NS3 NTPAse and RTPase activities as well as thr RNA helicase activity | Dengue virus type 2 | |
RNA | RNA-stimulated enzyme | Dengue virus type 2 |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and N-terminally truncated enzyme in Escherichia coli | Dengue virus type 2 |
Protein Variants | Comment | Organism |
---|---|---|
H51A | site-directed mutagenesis, the mutant has an inactivated protease domain showing enhanced RNA helicase compared to wild-type full-length enzyme | Dengue virus type 2 |
R184Q/K185N/R186G/K187N | construction of the N-terminally truncated mutant NS3DELTA180 containing a mutated RNA substrate biding motif, the mutant shows reduced RTPase activity | Dengue virus type 2 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Dengue virus type 2 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Dengue virus type 2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Dengue virus type 2 | RNA helicase activity | ADP + phosphate | - |
? | |
additional information | Dengue virus type 2 | nonstructural proteins NS3 and NS5 form complexes in infected mammalian cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus type 2 | - |
i.e. DEN2 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and N-terminally truncated enzyme from Escherichia coli | Dengue virus type 2 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specificities and activities of wild-type and mutant enzymes | Dengue virus type 2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | NTPase activity | Dengue virus type 2 | ADP + phosphate | - |
? | |
ATP + H2O | RNA helicase activity | Dengue virus type 2 | ADP + phosphate | - |
? | |
additional information | nonstructural proteins NS3 and NS5 form complexes in infected mammalian cells | Dengue virus type 2 | ? | - |
? | |
additional information | substrate specificity, bifunctional enzyme, NS3 is an RNA-stimulated nucleoside triphosphatase NTPase/RNA helicase and a 5'-RNA triphosphatase RTPase, overview, the full-length NS3 with or without NS2B cofactor domain exhibits a catalytically more efficient RNA helicase activity than the N-terminally-truncated NS3 helicase domain, suggesting that the protease domain enhances RNA helicase activity | Dengue virus type 2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | DEN2 nonstructural protein 3, NS3, has a serine protease domain and requires the hydrophilic domain of NS2B for activation | Dengue virus type 2 |
Synonyms | Comment | Organism |
---|---|---|
nonstructural protein 3 | - |
Dengue virus type 2 |
NS3 | - |
Dengue virus type 2 |
nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase | - |
Dengue virus type 2 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Dengue virus type 2 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Dengue virus type 2 |