BRENDA - Enzyme Database
show all sequences of 3.4.22.B50

Murine coronavirus ubiquitin-like domain is important for papain-like protease stability and viral pathogenesis

Mielech, A.M.; Deng, X.; Chen, Y.; Kindler, E.; Wheeler, D.L.; Mesecar, A.D.; Thiel, V.; Perlman, S.; Baker, S.C.; J. Virol. 89, 4907-4917 (2015) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Murine coronavirus
-
-
-
Synonyms
Synonyms
Commentary
Organism
PLP2
-
Murine coronavirus
General Information
General Information
Commentary
Organism
physiological function
changes within the Ubl domain, residues 785 to 787 of nonstructural protein 3, negatively affect protease activity. Ubl mutant viruses V787S and V785S replicate efficiently at 37°C but generate smaller plaques than wild-type virus, and V787S is defective for replication at higher temperatures. The proteases of the mutant viruses exhibit similar specific activities at 25°C but diplay significantly reduced thermal stability at 30°C, thereby reducing the total enzymatic activity. Infection of C57BL/6 mice with V787S is highly attenuated, yet it replicates sufficiently to elicit protective immunity
Murine coronavirus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
changes within the Ubl domain, residues 785 to 787 of nonstructural protein 3, negatively affect protease activity. Ubl mutant viruses V787S and V785S replicate efficiently at 37°C but generate smaller plaques than wild-type virus, and V787S is defective for replication at higher temperatures. The proteases of the mutant viruses exhibit similar specific activities at 25°C but diplay significantly reduced thermal stability at 30°C, thereby reducing the total enzymatic activity. Infection of C57BL/6 mice with V787S is highly attenuated, yet it replicates sufficiently to elicit protective immunity
Murine coronavirus
Other publictions for EC 3.4.22.B50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752517
Chu
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Kong
Structural view and substrate ...
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754545
Mielech
Murine coronavirus ubiquitin- ...
Murine coronavirus
J. Virol.
89
4907-4917
2015
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755638
van Kasteren
In vivo assessment of equine ...
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Chou
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Severe acute respiratory syndrome-related coronavirus
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8
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732257
Xing
The papain-like protease of po ...
porcine epidemic diarrhea virus
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1554-1567
2013
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732725
Dutta
Enhancement of proteolytic act ...
Tabernaemontana divaricata
PLoS ONE
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e62619
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van Kasteren
Deubiquitinase function of art ...
equine arteritis virus
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Chaudhuri
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718275
Wang
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Murine hepatitis virus
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Sun
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Ziebuhr
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Human coronavirus 229E
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Chen
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Human coronavirus NL63
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670702
Ratia
Severe acute respiratory syndr ...
Alphacoronavirus, Alphacoronavirus SARS-CoV
Proc. Natl. Acad. Sci. USA
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5717-5722
2006
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667619
Han
Papain-like protease 2 (PLP2) ...
Alphacoronavirus, Alphacoronavirus SARS-CoV
Biochemistry
44
10349-10359
2005
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1
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670084
Barretto
The papain-like protease of se ...
Alphacoronavirus, Alphacoronavirus SARS-CoV
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2005
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670085
Lindner
The papain-like protease from ...
Alphacoronavirus, Alphacoronavirus SARS-CoV
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15199-15208
2005
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668774
Kanjanahaluethai
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Coronavirus papain-like endope ...
Alphacoronavirus
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2004
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647692
Kanjanahaluethai
Identification of mouse hepati ...
Murine coronavirus
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2000
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Teng
Expression of murine coronavir ...
Murine coronavirus
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