Literature summary for 3.4.22.B50 extracted from

  • Xing, Y.; Chen, J.; Tu, J.; Zhang, B.; Chen, X.; Shi, H.; Baker, S.C.; Feng, L.; Chen, Z.
    The papain-like protease of porcine epidemic diarrhea virus negatively regulates type I interferon pathway by acting as a viral deubiquitinase (2013), J. Gen. Virol., 94, 1554-1567.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells porcine epidemic diarrhea virus

Engineering

Protein Variants Comment Organism
C1729A the mutant shows reduced interferon antagonistic activity compared to that of the wild type enzyme porcine epidemic diarrhea virus
H1888A the mutant almost completely loses interferon antagonistic activity compared to that of the wild type enzyme porcine epidemic diarrhea virus
D1901A the mutant almost completely loses interferon antagonistic activity compared to that of the wild type enzyme porcine epidemic diarrhea virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ubiquitin + H2O porcine epidemic diarrhea virus the enzyme processes both K-48 and K-63 linked polyubiquitin chains ?
-
 ?
ubiquitinated RIG-I + H2O porcine epidemic diarrhea virus
-
 deubiquitinated RIG-I + ubiquitin
-
 ?
ubiquitinated STING + H2O porcine epidemic diarrhea virus
-
 deubiquitinated STING + ubiquitin
-
 ?

Organism

Organism UniProt Comment Textmining
porcine epidemic diarrhea virus P0C6Y4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ubiquitin + H2O the enzyme processes both K-48 and K-63 linked polyubiquitin chains porcine epidemic diarrhea virus ?
-
 ?
ubiquitinated RIG-I + H2O
-
 porcine epidemic diarrhea virus deubiquitinated RIG-I + ubiquitin
-
 ?
ubiquitinated STING + H2O
-
 porcine epidemic diarrhea virus deubiquitinated STING + ubiquitin
-
 ?

Synonyms

Synonyms Comment Organism
PLP2
-
 porcine epidemic diarrhea virus

General Information

General Information Comment Organism
physiological function the enzyme strongly inhibits RIG-Iand STING-activated interferon expression. Papain-like protease 2 acts as a viral deubiquitinase to interfere with the RIG-I- and STING-mediated signalling pathway porcine epidemic diarrhea virus