BRENDA - Enzyme Database
show all sequences of 3.4.22.B50

PLP2 of mouse hepatitis virus A59 (MHV-A59) targets TBK1 to negatively regulate cellular type I interferon signaling pathway

Wang, G.; Chen, G.; Zheng, D.; Cheng, G.; Tang, H.; PLoS ONE 6, e17192 (2011) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in HEK-293T cells
Murine hepatitis virus
Engineering
Protein Variants
Commentary
Organism
C106A
inactive
Murine hepatitis virus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Murine hepatitis virus
5737
-
Organism
Organism
UniProt
Commentary
Textmining
Murine hepatitis virus
-
-
-
Synonyms
Synonyms
Commentary
Organism
papain-like protease domain 2
a catalytic domain of the nonstructural protein 3 of mouse hepatitis virus A59
Murine hepatitis virus
PLP2
-
Murine hepatitis virus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in HEK-293T cells
Murine hepatitis virus
Engineering (protein specific)
Protein Variants
Commentary
Organism
C106A
inactive
Murine hepatitis virus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Murine hepatitis virus
5737
-
General Information
General Information
Commentary
Organism
physiological function
papain-like protease domain 2 (PLP2) deubiquitinates TANK-binding kinase-1 (TBK1) and reduces its kinase activity, hence inhibits interferon-beta reporter activity and prevents interferon regulatory factor 3 (IRF3) nuclear translocation. The presence of PLP2 stabilizes the hypo-phosphorylated IRF3-TBK1 complex in a dose-dependent manner in the cytoplasm
Murine hepatitis virus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
papain-like protease domain 2 (PLP2) deubiquitinates TANK-binding kinase-1 (TBK1) and reduces its kinase activity, hence inhibits interferon-beta reporter activity and prevents interferon regulatory factor 3 (IRF3) nuclear translocation. The presence of PLP2 stabilizes the hypo-phosphorylated IRF3-TBK1 complex in a dose-dependent manner in the cytoplasm
Murine hepatitis virus
Other publictions for EC 3.4.22.B50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752517
Chu
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Kong
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Mielech
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Xing
The papain-like protease of po ...
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2013
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732725
Dutta
Enhancement of proteolytic act ...
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e62619
2013
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Wang
PLP2 of mouse hepatitis virus ...
Murine hepatitis virus
PLoS ONE
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e17192
2011
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1
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667619
Han
Papain-like protease 2 (PLP2) ...
Alphacoronavirus, Alphacoronavirus SARS-CoV
Biochemistry
44
10349-10359
2005
-
1
1
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5
9
-
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2
-
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5
-
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1
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12
1
2
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1
9
1
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1
1
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5
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9
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2
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1
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12
1
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1
9
1
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Barretto
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Alphacoronavirus, Alphacoronavirus SARS-CoV
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