Application | Comment | Organism |
---|---|---|
medicine | antiviral therapy | Alphacoronavirus |
Cloned (Comment) | Organism |
---|---|
cloned into modified version of the pBacPAK8/His2 vector and expressed in insect cells Sf9 | Alphacoronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | weakly inhibited by 70% at 0.01 mM | Alphacoronavirus | |
hydroxypridine-2-thione Zn | - |
Alphacoronavirus | |
additional information | not inhibited by Mg2+, Mn2+, Ca2+, Ni2+ and Co2+ at 0.01 mM, E64, leupeptin and antipain at 0.1 mM, N-ethylmaleimide and chymostatin at 1 mM and cystatin at 0.01 mg/mL respectively | Alphacoronavirus | |
N-ethyl-N-phenyldithiocarbaic acid Zn | - |
Alphacoronavirus | |
Zn2+ | - |
Alphacoronavirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 5.25 | Alphacoronavirus | |
0.064 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 6.05 | Alphacoronavirus | |
0.0654 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 6.82 | Alphacoronavirus | |
0.068 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 9.2 | Alphacoronavirus | |
0.0704 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 9.8 | Alphacoronavirus | |
0.0714 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 8.65 | Alphacoronavirus | |
0.072 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 8.01 | Alphacoronavirus | |
0.0772 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 7.42 | Alphacoronavirus | |
0.185 | - |
FRLKGGAPIKGV | - |
Alphacoronavirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
SDS-PAGE | Alphacoronavirus |
56700 | - |
gel filtration | Alphacoronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alphacoronavirus | - |
SARS-CoV | - |
Alphacoronavirus SARS-CoV | - |
SARS-CoV | - |
Purification (Comment) | Organism |
---|---|
gel filtration | Alphacoronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O | fluorogenic substrate | Alphacoronavirus | ? | - |
? | |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O | fluorogenic substrate | Alphacoronavirus SARS-CoV | ? | - |
? | |
BCoV substrate | - |
Alphacoronavirus | ? | - |
? | |
BCoV substrate | - |
Alphacoronavirus SARS-CoV | ? | - |
? | |
FRLKGGAPIKGV | 12 mer oligopeptide containing Gly818-Ala819, 100% cleavage | Alphacoronavirus | ? | - |
? | |
FRLKGGAPIKGV | 12 mer oligopeptide containing Gly818-Ala819, 100% cleavage | Alphacoronavirus SARS-CoV | ? | - |
? | |
ISLKGGKIVSTC | 12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage | Alphacoronavirus | ? | - |
? | |
ISLKGGKIVSTC | 12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage | Alphacoronavirus SARS-CoV | ? | - |
? | |
MHV substrate | - |
Alphacoronavirus | ? | - |
? | |
additional information | SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates | Alphacoronavirus | ? | - |
? | |
additional information | SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates | Alphacoronavirus SARS-CoV | ? | - |
? | |
RELNGGAVTRYV + H2O | 12 mer oligopeptide containing Gly180-Ala181, 5% cleavage | Alphacoronavirus | AVTYRV + RELNGG | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Alphacoronavirus |
Synonyms | Comment | Organism |
---|---|---|
papain-like protease | - |
Alphacoronavirus |
PLP2 | - |
Alphacoronavirus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
thermolabil, significantly reduced activity after incubation at temperatures higher than 37°C for 30 min | Alphacoronavirus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 5.25 | Alphacoronavirus | |
0.025 | - |
FRLKGGAPIKGV | - |
Alphacoronavirus | |
0.1 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 9.8 | Alphacoronavirus | |
0.198 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 6.05 | Alphacoronavirus | |
0.233 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 9.2 | Alphacoronavirus | |
0.33 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 7.42 | Alphacoronavirus | |
0.343 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 6.82 | Alphacoronavirus | |
0.363 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 8.01 | Alphacoronavirus | |
0.366 | - |
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine | at pH 8.65 | Alphacoronavirus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.6 | - |
Alphacoronavirus |