Papain-like protease 2 (PLP2) from severe acute respiratory syndrome coronavirus (SARS-CoV): expression, purification, characterization, and inhibition

Han, Y.S.; Chang, G.G.; Juo, C.G.; Lee, H.J.; Yeh, S.H.; Hsu, J.T.; Chen, X.; Biochemistry 44, 10349-10359 (2005)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

Cu2+

weakly inhibited by 70% at 0.01 mM

Alphacoronavirus

hydroxypridine-2-thione Zn

-

Alphacoronavirus

additional information

not inhibited by Mg2+, Mn2+, Ca2+, Ni2+ and Co2+ at 0.01 mM, E64, leupeptin and antipain at 0.1 mM, N-ethylmaleimide and chymostatin at 1 mM and cystatin at 0.01 mg/mL respectively

Alphacoronavirus

N-ethyl-N-phenyldithiocarbaic acid Zn

-

Alphacoronavirus

Zn2+

-

Alphacoronavirus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.021

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 5.25

Alphacoronavirus

0.064

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 6.05

Alphacoronavirus

0.0654

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 6.82

Alphacoronavirus

0.068

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 9.2

Alphacoronavirus

0.0704

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 9.8

Alphacoronavirus

0.0714

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 8.65

Alphacoronavirus

0.072

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 8.01

Alphacoronavirus

0.0772

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 7.42

Alphacoronavirus

0.185

-

FRLKGGAPIKGV

-

Alphacoronavirus

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O

fluorogenic substrate

667619

Alphacoronavirus

?

-

-

-

?

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O

fluorogenic substrate

667619

Alphacoronavirus SARS-CoV

?

-

-

-

?

BCoV substrate

-

667619

Alphacoronavirus

?

-

-

-

?

BCoV substrate

-

667619

Alphacoronavirus SARS-CoV

?

-

-

-

?

FRLKGGAPIKGV

12 mer oligopeptide containing Gly818-Ala819, 100% cleavage

667619

Alphacoronavirus

?

-

-

-

?

FRLKGGAPIKGV

12 mer oligopeptide containing Gly818-Ala819, 100% cleavage

667619

Alphacoronavirus SARS-CoV

?

-

-

-

?

ISLKGGKIVSTC

12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage

667619

Alphacoronavirus

?

-

-

-

?

ISLKGGKIVSTC

12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage

667619

Alphacoronavirus SARS-CoV

?

-

-

-

?

MHV substrate

-

667619

Alphacoronavirus

?

-

-

-

?

additional information

SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates

667619

Alphacoronavirus

?

-

-

-

?

additional information

SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates

667619

Alphacoronavirus SARS-CoV

?

-

-

-

?

RELNGGAVTRYV + H2O

12 mer oligopeptide containing Gly180-Ala181, 5% cleavage

667619

Alphacoronavirus

AVTYRV + RELNGG

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

0.023

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 5.25

Alphacoronavirus

0.025

-

FRLKGGAPIKGV

-

Alphacoronavirus

0.1

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 9.8

Alphacoronavirus

0.198

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 6.05

Alphacoronavirus

0.233

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 9.2

Alphacoronavirus

0.33

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 7.42

Alphacoronavirus

0.343

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 6.82

Alphacoronavirus

0.363

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 8.01

Alphacoronavirus

0.366

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 8.65

Alphacoronavirus

Inhibitors

Commentary

Organism

Structure

Cu2+

weakly inhibited by 70% at 0.01 mM

Alphacoronavirus

hydroxypridine-2-thione Zn

-

Alphacoronavirus

additional information

not inhibited by Mg2+, Mn2+, Ca2+, Ni2+ and Co2+ at 0.01 mM, E64, leupeptin and antipain at 0.1 mM, N-ethylmaleimide and chymostatin at 1 mM and cystatin at 0.01 mg/mL respectively

Alphacoronavirus

N-ethyl-N-phenyldithiocarbaic acid Zn

-

Alphacoronavirus

Zn2+

-

Alphacoronavirus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.021

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 5.25

Alphacoronavirus

0.064

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 6.05

Alphacoronavirus

0.0654

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 6.82

Alphacoronavirus

0.068

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 9.2

Alphacoronavirus

0.0704

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 9.8

Alphacoronavirus

0.0714

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 8.65

Alphacoronavirus

0.072

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 8.01

Alphacoronavirus

0.0772

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 7.42

Alphacoronavirus

0.185

-

FRLKGGAPIKGV

-

Alphacoronavirus

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O

fluorogenic substrate

667619

Alphacoronavirus

?

-

-

-

?

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O

fluorogenic substrate

667619

Alphacoronavirus SARS-CoV

?

-

-

-

?

BCoV substrate

-

667619

Alphacoronavirus

?

-

-

-

?

BCoV substrate

-

667619

Alphacoronavirus SARS-CoV

?

-

-

-

?

FRLKGGAPIKGV

12 mer oligopeptide containing Gly818-Ala819, 100% cleavage

667619

Alphacoronavirus

?

-

-

-

?

FRLKGGAPIKGV

12 mer oligopeptide containing Gly818-Ala819, 100% cleavage

667619

Alphacoronavirus SARS-CoV

?

-

-

-

?

ISLKGGKIVSTC

12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage

667619

Alphacoronavirus

?

-

-

-

?

ISLKGGKIVSTC

12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage

667619

Alphacoronavirus SARS-CoV

?

-

-

-

?

MHV substrate

-

667619

Alphacoronavirus

?

-

-

-

?

additional information

SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates

667619

Alphacoronavirus

?

-

-

-

?

additional information

SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates

667619

Alphacoronavirus SARS-CoV

?

-

-

-

?

RELNGGAVTRYV + H2O

12 mer oligopeptide containing Gly180-Ala181, 5% cleavage

667619

Alphacoronavirus

AVTYRV + RELNGG

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

0.023

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 5.25

Alphacoronavirus

0.025

-

FRLKGGAPIKGV

-

Alphacoronavirus

0.1

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 9.8

Alphacoronavirus

0.198

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 6.05

Alphacoronavirus

0.233

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 9.2

Alphacoronavirus

0.33

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 7.42

Alphacoronavirus

0.343

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 6.82

Alphacoronavirus

0.363

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 8.01

Alphacoronavirus

0.366

-

Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine

at pH 8.65

Alphacoronavirus

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Protein Variants

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

kcat/KM [mM/s]

kcat/KM [mM/s] (protein specific)

752517

Chu

Porcine epidemic diarrhea vir ...

porcine epidemic diarrhea virus

Antiviral Res.

158

199-205

2018

-

-

1

1

1

-

2

4

-

-

1

-

-

1

-

-

-

-

-

-

-

-

3

1

-

-

-

-

4

-

-

-

-

1

-

2

-

-

1

-

1

1

-

2

2

1

4

-

-

1

-

-

-

-

-

-

-

-

-

3

1

-

-

-

4

-

-

-

-

-

-

-

-

4

4

731149

Baez-Santos

The SARS-coronavirus papain-li ...

Severe acute respiratory syndrome-related coronavirus

Antiviral Res.

115

21-38

2015

-

1

-

1

1

-

20

-

-

2

-

-

-

5

-

-

-

1

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

1

-

16

-

1

-

-

1

1

-

16

20

1

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

2

-

-

-

732186

Kong

Structural view and substrate ...

infectious bronchitis virus

J. Biol. Chem.

290

7160-7168

2015

-

-

1

1

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-

2

-

-

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1

-

1

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1

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-

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4

-

1

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1

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1

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2

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1

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1

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-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

754545

Mielech

Murine coronavirus ubiquitin- ...

Murine coronavirus

J. Virol.

89

4907-4917

2015

-

-

-

-

-

-

-

-

-

-

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-

1

-

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-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

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-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

755638

van Kasteren

In vivo assessment of equine ...

equine arteritis virus, equine arteritis virus Bucyrus

Vet. Microbiol.

178

132-137

2015

-

1

-

-

1

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

-

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-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

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-

-

-

-

-

-

-

-

-

-

-

731069

Chou

Structural basis for catalysis ...

Severe acute respiratory syndrome-related coronavirus

Acta Crystallogr. Sect. D

70

572-581

2014

-

-

1

1

9

-

3

9

-

-

-

-

-

3

-

-

1

-

-

-

-

-

2

-

3

1

-

-

8

1

-

-

-

2

-

-

-

-

1

-

1

9

-

-

3

2

9

-

-

-

-

-

-

-

1

-

-

-

-

2

-

1

-

-

8

1

-

-

-

-

3

3

-

8

8

732257

Xing

The papain-like protease of po ...

porcine epidemic diarrhea virus

J. Gen. Virol.

94

1554-1567

2013

-

-

1

-

3

-

-

-

-

-

-

3

-

1

-

-

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-

-

-

-

-

3

-

1

-

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-

-

-

-

-

-

-

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-

1

-

-

3

-

-

-

-

-

-

-

-

3

-

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-

-

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-

-

3

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

732725

Dutta

Enhancement of proteolytic act ...

Tabernaemontana divaricata

PLoS ONE

8

e62619

2013

1

-

1

-

3

-

1

4

-

-

1

-

-

3

-

-

1

-

1

-

4

-

3

1

2

1

-

1

4

1

1

-

-

-

-

4

1

-

1

-

-

3

-

4

1

-

4

-

-

1

-

-

-

-

1

1

-

4

-

3

1

1

-

1

4

1

1

-

-

-

-

-

-

4

4

732827

van Kasteren

Deubiquitinase function of art ...

equine arteritis virus

Proc. Natl. Acad. Sci. USA

110

E838-E847

2013

-

-

1

1

4

-

1

-

-

-

-

1

-

1

-

-

1

-

-

-

-

-

3

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

1

4

-

-

1

-

-

-

-

-

1

-

-

-

1

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

2

2

-

6

6

718000

Chaudhuri

Comparison of SARS and NL63 pa ...

Human coronavirus NL63

J. Mol. Biol.

414

272-288

2011

-

1

-

-

-

-

-

-

-

-

-

3

-

1

-

-

-

-

-

-

-

-

3

-

1

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

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3

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-

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-

-

-

3

-

-

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-

-

-

-

-

-

-

-

-

-

-

-

718275

Wang

PLP2 of mouse hepatitis virus ...

Murine hepatitis virus

PLoS ONE

6

e17192

2011

-

-

1

-

1

-

-

-

1

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

718347

Sun

-

Deubiquitinase activity and re ...

Human coronavirus NL63

Prog. Biochem. Biophys.

37

871-880

2010

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

681685

Ziebuhr

Human coronavirus 229E papain- ...

Human coronavirus 229E

J. Virol.

81

3922-3932

2007

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

1

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

681691

Chen

Proteolytic processing and deu ...

Human coronavirus NL63

J. Virol.

81

6007-6018

2007

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

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