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Literature summary for 3.4.22.B14 extracted from

  • Bekes, M.; Rut, W.; Kasperkiewicz, P.; Mulder, M.; Ovaa, H.; Drag, M.; Lima, C.; Huang, T.
    SARS hCoV papain-like protease is a unique Lys48 linkage-specific di-distributive deubiquitinating enzyme (2015), Biochem. J., 468, 215-226 .
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Middle East respiratory syndrome-related coronavirus
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Severe acute respiratory syndrome-related coronavirus
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human, i.e. SARS-CoV
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ISG15-7-amido-4-methylcoumarin + H2O
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Severe acute respiratory syndrome-related coronavirus ISG15 + 7-amino-4-methylcoumarin
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?
ISG15-7-amido-4-methylcoumarin + H2O
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Middle East respiratory syndrome-related coronavirus ISG15 + 7-amino-4-methylcoumarin
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?
additional information enzyme is able to cleave ubiquitin mutant L73P in K6-, K11-, K48- and K63-linked di-ubiquitin chains. Cleavage rates with MERS PLpro are nearly identical against ubiquitin chains of various lengths Middle East respiratory syndrome-related coronavirus ?
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?
additional information MERS PLpro has an absolute requirement for Gly in the P2 position, but can accommodate a wide variety of hydrophobic amino acids in both the P3 and P4 positions. The most preferred amino acid in P3 is Leu, followed by Arg, Lys and hydrophobic residues. The most preferred amino acid in P4 is also Leu, followed by mostly hydrophobic residues Middle East respiratory syndrome-related coronavirus ?
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?
additional information SARS PLpro recognizes a K48-linked tri-Ub as a minimal unit for ubiquitin chain processing Severe acute respiratory syndrome-related coronavirus ?
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?
tetra-ubiquitin + H2O
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Severe acute respiratory syndrome-related coronavirus 2 di-ubiquitin
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?
ubiquitin-7-amido-4-methylcoumarin + H2O
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Severe acute respiratory syndrome-related coronavirus ubiquitin + 7-amino-4-methylcoumarin
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?
ubiquitin-7-amido-4-methylcoumarin + H2O
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Middle East respiratory syndrome-related coronavirus ubiquitin + 7-amino-4-methylcoumarin
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?

General Information

General Information Comment Organism
physiological function MERS PLpro is both a deubiquitinating and a deISGylating enzyme (ISG15 i.e. ubiquitin-like modifier interferon stimulated gene 15). MERS PLpro shows broad linkage specificity for the cleavage of polyUb chains and cleaves polyUb chains one ubiquitin at a time. Comparison with SARS PLpro Middle East respiratory syndrome-related coronavirus
physiological function SARS PLpro prefers to cleave Lys48-linked polyUb chains and needs a di-Ub moiety as a minimal recognition element. Comaprison with MERS PLpro enzyme Severe acute respiratory syndrome-related coronavirus