Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli by using a T7 RNA polymerase promoter system or as a maltose-binding protein fusion protein. With both overexpression systems the recombinant PLP-1 exhibits trans cleavage activity. When PLP-1 is expressed as a polypeptide that includes additional viral sequences at the carboxyl terminus of the predicted PLP-1 domain, a fivefold increase in proteolytic activity is observed | Murine coronavirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ORF 1a polyprotein + H2O | Murine coronavirus | cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle | ? | - |
? | |
ORF 1a polyprotein + H2O | Murine coronavirus JHM | cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Murine coronavirus | P0C6V1 | i.e. mouse hepatitis virus | - |
Murine coronavirus JHM | P0C6V1 | i.e. mouse hepatitis virus | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Murine coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da | Murine coronavirus | ? | - |
? | |
additional information | viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da | Murine coronavirus JHM | ? | - |
? | |
ORF 1a polyprotein + H2O | cleavage in cis and in trans | Murine coronavirus | ? | - |
? | |
ORF 1a polyprotein + H2O | cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle | Murine coronavirus | ? | - |
? | |
ORF 1a polyprotein + H2O | cleavage in cis and in trans | Murine coronavirus JHM | ? | - |
? | |
ORF 1a polyprotein + H2O | cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle | Murine coronavirus JHM | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
reaction is significantly more efficient at 22°C than when carried out at 30°C | Murine coronavirus |