Literature summary for 3.4.22.69 extracted from

Kanitz, M.; Blanck, S.; Heine, A.; Gulyaeva, A.A.; Gorbalenya, A.E.; Ziebuhr, J.; Diederich, W.E.
Structural basis for catalysis and substrate specificity of a 3C-like cysteine protease from a mosquito mesonivirus (2019), Virology, 533, 21-33 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization of free enzyme and the enzyme/peptide aldehyde inhibitor(benzoyl-YYNQ-H) complex by hanging-drop method. The 3CLpro structure (refined to 1.94 A) shows that the protein forms dimers. The monomers are comprised of N-terminal domains I and II, which adopt a chymotrypsin-like fold, and a C-terminal alpha-helical domain III. The catalytic Cys-His dyad is assisted by a complex network of interactions involving a water molecule that mediates polar contacts between the catalytic His and a conserved Asp located in the domain II-III junction and is suitably positioned to stabilize the developing positive charge of the catalytic His in the transition state during catalysis	Alphamesonivirus 1

Crystallization (Comment)

Organism

crystallization of free enzyme and the enzyme/peptide aldehyde inhibitor(benzoyl-YYNQ-H) complex by hanging-drop method. The 3CLpro structure (refined to 1.94 A) shows that the protein forms dimers. The monomers are comprised of N-terminal domains I and II, which adopt a chymotrypsin-like fold, and a C-terminal alpha-helical domain III. The catalytic Cys-His dyad is assisted by a complex network of interactions involving a water molecule that mediates polar contacts between the catalytic His and a conserved Asp located in the domain II-III junction and is suitably positioned to stabilize the developing positive charge of the catalytic His in the transition state during catalysis

Alphamesonivirus 1

Inhibitors

Inhibitors	Comment	Organism	Structure
benzoyl-YYNQ-H	-	Alphamesonivirus 1

Organism

Organism	UniProt	Comment	Textmining
Alphamesonivirus 1	F8RL29	isolate Aedes harrisoni	-

Purification (Commentary)

Purification (Comment)	Organism
-	Alphamesonivirus 1

Synonyms

Synonyms	Comment	Organism
3C-like cysteine protease	-	Alphamesonivirus 1

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Release 2023.1 (January 2023)