Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.69 extracted from

  • Okamoto, D.N.; Oliveira, L.C.; Kondo, M.Y.; Cezari, M.H.; Szeltner, Z.; Juhasz, T.; Juliano, M.A.; Polgar, L.; Juliano, L.; Gouvea, I.E.
    Increase of SARS-CoV 3CL peptidase activity due to macromolecular crowding effects in the milieu composition (2010), Biol. Chem., 391, 1461-1468.
    View publication on PubMed
Search for more literature for 3.4.22.69

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 10% (w/v) bovine serum albumin improves the hydrolytic rate by a factor of 2 Severe acute respiratory syndrome-related coronavirus
PEG6000 3fold activation in the presence of 10% (w/v) PEG6000 Severe acute respiratory syndrome-related coronavirus
Sodium sulfate 1 M sodium sulfate stimulates activity Severe acute respiratory syndrome-related coronavirus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.0011
-
 Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.0013
-
 Abz-VLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.0013
-
 Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.0016
-
 Abz-SAVLQSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.0017
-
 Abz-LQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.002
-
 Abz-SAVLQSGFRKMAK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.0024
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.011
-
 Abz-QTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.0136
-
 Abz-SAVLQSGK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.016
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.023
-
 Abz-LQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.033
-
 Abz-VLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the enzyme activity is unaffected by Brij-35, Triton X-100 and Tween 20 or SDS Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Resource-Q column chromatography, gel filtration Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-LQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-LQ + SGFRK(Dnp)NH2
-
 ?
Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-LYQPPQTSITSAVLQ + SGFRK(Dnp)NH2
-
 ?
Abz-QTSITSAVLQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-QTSITSAVLQ + SGFRK(Dnp)NH2
-
 ?
Abz-SAVLQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGFRK(Dnp)NH2
-
 ?
Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGFRKMAFPSGK(Dnp)NH2
-
 ?
Abz-SAVLQSGFRKMAK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGFRKMAK(Dnp)NH2
-
 ?
Abz-SAVLQSGK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGK(Dnp)NH2
-
 ?
Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-SGADVLYQPPQTSITSAVLQ + SGFRK(Dnp)NH2
-
 ?
Abz-VLQSGFRK(Dnp)NH2 + H2O
-
 Severe acute respiratory syndrome-related coronavirus Abz-VLQ + SGFRK(Dnp)NH2
-
 ?

Synonyms

Synonyms Comment Organism
3cLpro
-
 Severe acute respiratory syndrome-related coronavirus
SARS-CoV 3CL peptidase
-
 Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
 Abz-LQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.004
-
 Abz-SAVLQSGK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.02
-
 Abz-LQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.04
-
 Abz-VLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.07
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.11
-
 Abz-SAVLQSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.28
-
 Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.38
-
 Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.6
-
 Abz-QTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.77
-
 Abz-VLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
2.9
-
 Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3
-
 Abz-SAVLQSGFRKMAK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
3.3
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Severe acute respiratory syndrome-related coronavirus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5
-
 Severe acute respiratory syndrome-related coronavirus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.13
-
 Abz-LQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
0.31
-
 Abz-SAVLQSGK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
1.2
-
 Abz-VLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
4.4
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
12
-
 Abz-LQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
69
-
 Abz-SAVLQSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
345
-
 Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
545
-
 Abz-QTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
560
-
 Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
592
-
 Abz-VLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
1440
-
 Abz-SAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
1500
-
 Abz-SAVLQSGFRKMAK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus
2250
-
 Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C Severe acute respiratory syndrome-related coronavirus