Crystallization (Comment) | Organism |
---|---|
SARS-CoV Mpro in complex with Cm-FF-H, Ac-ESTLQ-H, Ac-DSFDQ-H, Ac-NSTSQ-H, Ac-NSFSQ-H, or Ac-ESTLQ-H, sitting drop vapor diffusion method, using 8% (w/v) PEG 6000, 0.1 M MES (pH 6.0), 3% (v/v) 2-methyl-2,4-pentanediol, and 3% (v/v) DMSO, at 20°C | Severe acute respiratory syndrome-related coronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-acetyl-DSFDQ | micromolar inhibitor | Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-ESTLQ | micromolar inhibitor | Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-NSFSQ | micromolar inhibitor | Severe acute respiratory syndrome-related coronavirus | |
N-acetyl-NSTSQ | micromolar inhibitor | Severe acute respiratory syndrome-related coronavirus | |
N-[(2S)-1-oxo-3-phenylpropan-2-yl]-Na-[(2E)-3-phenylprop-2-enoyl]-L-phenylalaninamide | - |
Severe acute respiratory syndrome-related coronavirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0245 | - |
Dabcyl-KTSAVLQSGFRKME-EDANS-amide | in 40 mM Tris-HCl buffer, pH 7.3, temperature not specified in the publication | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | P0C6U8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dabcyl-KTSAVLQSGFRKME-EDANS-amide + H2O | - |
Severe acute respiratory syndrome-related coronavirus | Dabcyl-KTSAVLQ + SGFRKME-EDANS-amide | - |
? | |
additional information | no activity towards SWTSAVKQSGFRKWA, SWTSAVRQSGFRKWA, SWTSAVEQSGFRKWA, and SWTSAVDQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | ? | - |
? | |
additional information | the substrate specificity of the enzyme requires glutamine in the P1 position and a large hydrophobic residue in the P2 position | Severe acute respiratory syndrome-related coronavirus | ? | - |
? | |
SWTSAVAQSGFRKWA + H2O | less than 10% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVAQ + SGFRKWA | - |
? | |
SWTSAVCQSGFRKWA + H2O | less than 1% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVCQ + SGFRKWA | - |
? | |
SWTSAVFQSGFRKWA + H2O | about 70% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVFQ + SGFRKWA | - |
? | |
SWTSAVHQSGFRKWA + H2O | less than 5% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVHQ + SGFRKWA | - |
? | |
SWTSAVIQSGFRKWA + H2O | about 45% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVIQ + SGFRKWA | - |
? | |
SWTSAVLQSGFRKWA + H2O | 100% activity | Severe acute respiratory syndrome-related coronavirus | SWTSAVLQ + SGFRKWA | - |
? | |
SWTSAVMQSGFRKWA + H2O | about 58% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVMQ + SGFRKWA | - |
? | |
SWTSAVNQSGFRKWA + H2O | less than 1% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVNQ + SGFRKWA | - |
? | |
SWTSAVPQSGFRKWA + H2O | about 10% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVPQ + SGFRKWA | - |
? | |
SWTSAVQQSGFRKWA + H2O | less than 5% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVQQ + SGFRKWA | - |
? | |
SWTSAVSQSGFRKWA + H2O | less than 1% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVSQ + SGFRKWA | - |
? | |
SWTSAVTQSGFRKWA + H2O | less than 5% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVTQ + SGFRKWA | - |
? | |
SWTSAVVQSGFRKWA + H2O | about 55% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVVQ + SGFRKWA | - |
? | |
SWTSAVWQSGFRKWA + H2O | less than 5% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVWQ + SGFRKWA | - |
? | |
SWTSAVYQSGFRKWA + H2O | less than 5% activity compared to SWTSAVLQSGFRKWA | Severe acute respiratory syndrome-related coronavirus | SWTSAVYQ + SGFRKWA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SARS coronavirus main protease | - |
Severe acute respiratory syndrome-related coronavirus |
SARS-CoV Mpro | - |
Severe acute respiratory syndrome-related coronavirus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00224 | - |
N-[(2S)-1-oxo-3-phenylpropan-2-yl]-Na-[(2E)-3-phenylprop-2-enoyl]-L-phenylalaninamide | in 40 mM Tris-HCl buffer, pH 7.3, temperature not specified in the publication | Severe acute respiratory syndrome-related coronavirus | |
0.00827 | - |
N-acetyl-ESTLQ | in 40 mM Tris-HCl buffer, pH 7.3, temperature not specified in the publication | Severe acute respiratory syndrome-related coronavirus | |
0.04098 | - |
N-acetyl-NSFSQ | in 40 mM Tris-HCl buffer, pH 7.3, temperature not specified in the publication | Severe acute respiratory syndrome-related coronavirus | |
0.04124 | - |
N-acetyl-DSFDQ | in 40 mM Tris-HCl buffer, pH 7.3, temperature not specified in the publication | Severe acute respiratory syndrome-related coronavirus | |
0.07273 | - |
N-acetyl-NSTSQ | in 40 mM Tris-HCl buffer, pH 7.3, temperature not specified in the publication | Severe acute respiratory syndrome-related coronavirus |