Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | SARS coronavirus Tor2 |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystals of S139A mutant are grown from the mother liquor containing 0.1 M MES pH 6.0, 10% (w/v) PEG 6000, 1 mM dithiohtreitol, and 5% (v/v) DMSO, crystals of F140A are grown at three pH values in 0.1 M MES pH 6.0/0.1 M MES pH 6.5/0.1 M Tris pH 7.6, with 10% (w/v) PEG 6000, 1 mM dithiothreitol, and 5% (v/v) DMSO | SARS coronavirus Tor2 |
Protein Variants | Comment | Organism |
---|---|---|
F140A | mutant F140A is a dimer with the most collapsed active pocket discovered so far, well-reflecting the stabilizing role of this residue, the mutant enzyme is completely inactive | SARS coronavirus Tor2 |
S139A | mutant S139A is a monomer that still retains a small fraction of dimer in solution, which may account for its remaining activity | SARS coronavirus Tor2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
SARS coronavirus Tor2 | P0C6U8 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography and Superdex 75 gel filtration | SARS coronavirus Tor2 |
Subunits | Comment | Organism |
---|---|---|
homodimer | only the dimeric enzyme is active | SARS coronavirus Tor2 |
Synonyms | Comment | Organism |
---|---|---|
3C-like protease | - |
SARS coronavirus Tor2 |
3cLpro | - |
SARS coronavirus Tor2 |
General Information | Comment | Organism |
---|---|---|
physiological function | 3CLpro is vital for SARS-coronavirus replication | SARS coronavirus Tor2 |