Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Severe acute respiratory syndrome-related coronavirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.286 | - |
TFTRLQSLENV | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
0.549 | - |
FYPKLQASQAW | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
0.583 | - |
SGVTFQGKFKK | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
1.15 | - |
TSAVLQSGFRK-NH2 | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
1.44 | - |
ATVRLQAGNAT | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
1.94 | - |
PHTVLQAVGAC | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Severe acute respiratory syndrome-related coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATVRLQAGNAT + H2O | - |
Severe acute respiratory syndrome-related coronavirus | ATVRLQ + AGNAT | - |
? | |
FYPKLQASQAW + H2O | - |
Severe acute respiratory syndrome-related coronavirus | FYPKLQ + ASQAW | - |
? | |
KVATVQSKMSD + H2O | weak activity | Severe acute respiratory syndrome-related coronavirus | KVATVQ + SKMSD | - |
? | |
additional information | cuts the 11 peptides covering all of the 11 cleavage sites on the viral polyprotein with different efficiency | Severe acute respiratory syndrome-related coronavirus | ? | - |
? | |
NRATLQAIASE + H2O | weak activity | Severe acute respiratory syndrome-related coronavirus | NRATLQ + AIASE | - |
? | |
NVATLQAENVT + H2O | weak activity | Severe acute respiratory syndrome-related coronavirus | NVATLQ + AENVT | - |
? | |
PHTVLQAVGAC + H2O | - |
Severe acute respiratory syndrome-related coronavirus | PHTVLQ + AVGAC | - |
? | |
REPLMQSADAS + H2O | weak activity | Severe acute respiratory syndrome-related coronavirus | REPLMQ + SADAS | - |
? | |
SAVKLQNNELS + H2O | weak activity | Severe acute respiratory syndrome-related coronavirus | SAVKLQ + NNELS | - |
? | |
SGVTFQGKFKK + H2O | highest cleavage efficiency. The two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive ones | Severe acute respiratory syndrome-related coronavirus | SGVTFQ + GKFKK | - |
? | |
TFTRLQSLENV + H2O | - |
Severe acute respiratory syndrome-related coronavirus | TFTRLQ + SLENV | - |
? | |
TSAVLQSGFRK-NH2 + H2O | highest cleavage efficiency. The two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive ones | Severe acute respiratory syndrome-related coronavirus | TSAVLQ + SGFRK-NH2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | a mixture of monomer and dimer at a protein concentration of 4 mg/ml and mostly monomer at 0.2 mg/ml. The dimer may be the biological functional form of the protein | Severe acute respiratory syndrome-related coronavirus |
monomer | a mixture of monomer and dimer at a protein concentration of 4 mg/ml and mostly monomer at 0.2 mg/ml. The dimer may be the biological functional form of the protein | Severe acute respiratory syndrome-related coronavirus |
Synonyms | Comment | Organism |
---|---|---|
SARS 3C-like proteinase | - |
Severe acute respiratory syndrome-related coronavirus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
61 | - |
Tm-value, sigmoid denaturation curve | Severe acute respiratory syndrome-related coronavirus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.847 | - |
TFTRLQSLENV | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
1.57 | - |
FYPKLQASQAW | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
1.68 | - |
PHTVLQAVGAC | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
2.55 | - |
SGVTFQGKFKK | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.29 | - |
ATVRLQAGNAT | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
12.2 | - |
TSAVLQSGFRK-NH2 | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
substrate: TSAVLQSGFRK-NH2 | Severe acute respiratory syndrome-related coronavirus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6: about 50% of maximal activity, pH 9: about 65% of maximal activity, substrate: TSAVLQSGFRK-NH2 | Severe acute respiratory syndrome-related coronavirus |